IR@PKUHSC  > 北京大学第一临床医学院  > 临床药理研究所
学科主题临床医学
Molecular characterization of erm(B)- and mef(E)-mediated erythromycin-resistant Streptococcus pneumoniae in China and complete DNA sequence of Tn2010
Li, Y.1,2; Tomita, H.1; Lv, Y.2; Liu, J.2; Xue, F.2; Zheng, B.1,2; Ike, Y.1,3
关键词CC271 China erm(B) erythromycin resistance mef(E) Streptococcus pneumoniae Tn2010 Tn6002
刊名JOURNAL OF APPLIED MICROBIOLOGY
2011
DOI10.1111/j.1365-2672.2010.04875.x
110期:1页:254-265
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biotechnology & Applied Microbiology ; Microbiology
研究领域[WOS]Biotechnology & Applied Microbiology ; Microbiology
关键词[WOS]FIELD GEL-ELECTROPHORESIS ; MACROLIDE RESISTANCE ; CONJUGATIVE TRANSPOSONS ; NUCLEOTIDE-SEQUENCE ; GENETIC ELEMENTS ; MULTIPLEX PCR ; MEF(A) ; DETERMINANTS ; ASSOCIATION ; PNEUMOCOCCI
英文摘要

Aims:

To characterize the erm(B)- and mef(E)-mediated erythromycin-resistant Streptococcus pneumoniae clinical isolates obtained from ten hospitals located different cities in China.

Methods and Results:

Totally 83 S. pneumoniae were collected, and eighteen representative strains of 66 strains that exhibited erythromycin resistance were used for further characterization by antibiograms, serotyping, PFGE, MLST, DNA sequencing of the macrolide-resistance elements and mapping of the elements on the chromosome. Twelve isolates showed a high-level resistance to erythromycin, and six other isolates showed a low-level resistance to erythromycin. Thirteen isolates harboured a Tn2010 transposon (26 center dot 4 kbp) encoding the erm(B), tet(M) and mef(E) genes and were classified into three types by Tn2010 structures. The remaining five isolates harboured a Tn6002 transposon (20 center dot 9 kbp) encoding the erm(B) and tet(M) genes and were classified into three types by Tn6002 locations on the chromosome. Three of the Tn6002 elements were located within the Tn5252-like element, implying that these composed a large mobile element. The MLST analyses showed that several clones had been disseminated and that the CC271 strains carrying the Tn2010 element expressing the high-level resistance to erythromycin were predominant in China. Four new MLST strains, which were designated as ST3262, ST3263, ST3397 and ST3398 were also identified.

Conclusions:

The erythromycin resistance determinant of S. pneumoniae that had been isolated in China was located in Tn2010 or the Tn6002 element and several clones had been disseminated, and the CC271 strains carrying the Tn2010 element expressing the high-level resistance to erythromycin were predominant in China.

Significance and Impact of the Study:

This is the first molecular analysis of erythromycin-resistant Streptococcus pneumoniae clinical isolates in China, and the first report of the complete nucleotide sequence of Tn2010 (26 390 bp).

语种英语
WOS记录号WOS:000285207600028
引用统计
被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62313
专题北京大学第一临床医学院_临床药理研究所
作者单位1.Gunma Univ, Grad Sch Med, Dept Bacteriol, Gunma 3718511, Japan
2.Peking Univ, Hosp 1, Inst Clin Pharmacol, Beijing 100871, Peoples R China
3.Gunma Univ, Grad Sch Med, Drug Resistance Bacteria Lab, Gunma 3718511, Japan
推荐引用方式
GB/T 7714
Li, Y.,Tomita, H.,Lv, Y.,et al. Molecular characterization of erm(B)- and mef(E)-mediated erythromycin-resistant Streptococcus pneumoniae in China and complete DNA sequence of Tn2010[J]. JOURNAL OF APPLIED MICROBIOLOGY,2011,110(1):254-265.
APA Li, Y..,Tomita, H..,Lv, Y..,Liu, J..,Xue, F..,...&Ike, Y..(2011).Molecular characterization of erm(B)- and mef(E)-mediated erythromycin-resistant Streptococcus pneumoniae in China and complete DNA sequence of Tn2010.JOURNAL OF APPLIED MICROBIOLOGY,110(1),254-265.
MLA Li, Y.,et al."Molecular characterization of erm(B)- and mef(E)-mediated erythromycin-resistant Streptococcus pneumoniae in China and complete DNA sequence of Tn2010".JOURNAL OF APPLIED MICROBIOLOGY 110.1(2011):254-265.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Li, Y.]的文章
[Tomita, H.]的文章
[Lv, Y.]的文章
百度学术
百度学术中相似的文章
[Li, Y.]的文章
[Tomita, H.]的文章
[Lv, Y.]的文章
必应学术
必应学术中相似的文章
[Li, Y.]的文章
[Tomita, H.]的文章
[Lv, Y.]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。