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学科主题临床医学
Novel Therapeutic Approach for Neurogenic Erectile Dysfunction: Effect of Neurotrophic Tyrosine Kinase Receptor Type 1 Monoclonal Antibody
Lin, Guiting1; Li, Huixi1,2,3; Zhang, Xiaoyu1,2,3; Wang, Jianwen1,4; Zaid, Uwais1; Sanford, Melissa T.1; Tu, Victor1; Wu, Alex1; Wang, Lin1,2,3; Tian, Fei5; Kotanides, Helen5; Krishnan, Venkatesh5; Wang, Guifang1; Ning, Hongxiu1; Banie, Lia1; Lin, Ching-Shwun1; Deng, Gary G.5; Lue, Tom F.1
关键词Cavernous nerve injury Neurogenic erectile dysfunction of peripheral origin Tyrosine kinase receptor type 1 monoclonal antibody Nerve regeneration Sexual behavior
刊名EUROPEAN UROLOGY
2015-04-01
DOI10.1016/j.eururo.2014.10.013
67期:4页:716-726
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Urology & Nephrology
研究领域[WOS]Urology & Nephrology
关键词[WOS]CAVERNOUS NERVE INJURY ; MAJOR PELVIC GANGLION ; MATING-BEHAVIOR ; GROWTH-FACTOR ; RAT MODEL ; PENILE ERECTION ; DIABETIC-RATS ; IMPROVES ERECTILE ; PROSTATE-CANCER ; TRKA
英文摘要

Background: Erectile dysfunction (ED) is a major health issue in aged populations, and neurogenic ED is particularly difficult to treat. Novel therapeutic approaches are needed for treatment of neurogenic ED of peripheral origin.

Objective: To investigate the therapeutic effects of a neurotrophic tyrosine kinase receptor type 1 monoclonal antibody (TrkA-mAb) on erectile function and sexual behavior in a rat model of cavernous nerve injury (CNI).

Design, setting, and participants: In one experiment, 84 male rats were randomly assigned to seven groups. The groups underwent either CNI or sham surgery, subsequent injection into the major pelvic ganglion IMPG) of phosphate-buffered saline (PBS), an immunoglobulin G (IgG) control, or TrkA-mAb, and then intracavernosal (IC) injection of either PBS or varying TrkA-mAb concentrations immediately after surgery and then 1wk later. Erectile function was assessed and histologic/molecular analyses were performed at 6 wk after surgery. In a second experiment, 36 male rats were randomly divided into three groups. The groups underwent CNI or sham surgery and then IC injection of PBS, IgG, or TrkA-mAb immediately after surgery and for 5 wk thereafter. At 6 wk after surgery, the performance of the rats in sexual behavior tests was videotaped.

Intervention: CNI or sham surgery; IMPG of PBS, IgG, or TrkA-mAb; IC injection of PBS or TrkA-mAb.

Outcome measurements and statistical analysis: The intracavernous pressure response to cavernous nerve electrostimulation was measured and midpenile cross-sections were histologically examined. Western blotting (WB) of cavernous tissue protein was performed. Rats were assessed for chasing, mounting, intromission, and ejaculation behaviors during sexual behavior tests. The data were analyzed using one-way analysis of variance followed by the Tukey-Kramer t test.

语种英语
WOS记录号WOS:000349910900031
资助机构Eli Lilly and company
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62314
专题北京大学第一临床医学院_泌尿外科
北京大学口腔医学院_中心实验室
作者单位1.Peking Univ, Hosp 1, Dept Urol, Beijing 100871, Peoples R China
2.Peking Univ, Inst Urol, Beijing 100871, Peoples R China
3.Eli Lilly & Co, Indianapolis, IN 46285 USA
4.Univ Calif San Francisco, Sch Med, Dept Urol, Knuppe Mol Urol Lab, San Francisco, CA 94143 USA
5.Capital Med Univ, Sch Med, Dept Urol, Beijing, Peoples R China
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GB/T 7714
Lin, Guiting,Li, Huixi,Zhang, Xiaoyu,et al. Novel Therapeutic Approach for Neurogenic Erectile Dysfunction: Effect of Neurotrophic Tyrosine Kinase Receptor Type 1 Monoclonal Antibody[J]. EUROPEAN UROLOGY,2015,67(4):716-726.
APA Lin, Guiting.,Li, Huixi.,Zhang, Xiaoyu.,Wang, Jianwen.,Zaid, Uwais.,...&Lue, Tom F..(2015).Novel Therapeutic Approach for Neurogenic Erectile Dysfunction: Effect of Neurotrophic Tyrosine Kinase Receptor Type 1 Monoclonal Antibody.EUROPEAN UROLOGY,67(4),716-726.
MLA Lin, Guiting,et al."Novel Therapeutic Approach for Neurogenic Erectile Dysfunction: Effect of Neurotrophic Tyrosine Kinase Receptor Type 1 Monoclonal Antibody".EUROPEAN UROLOGY 67.4(2015):716-726.
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