IR@PKUHSC  > 北京大学第一临床医学院
学科主题临床医学
Transcriptional activation of steroidogenic factor-1 by hypomethylation of the 5 ′ CpG island in endometriosis
Xue, Qing; Lin, Zhihong; Yin, Ping; Milad, Magdy P.; Cheng, You-Hong; Confino, Edmond; Reierstad, Scott; Bulun, Serdar E.
刊名JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
2007-08-01
DOI10.1210/jc.2007-0494
92期:8页:3261-3267
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Endocrinology & Metabolism
研究领域[WOS]Endocrinology & Metabolism
关键词[WOS]DNA METHYLATION ; EPIGENETIC MODIFICATIONS ; AROMATASE EXPRESSION ; GENE-EXPRESSION ; KEY REGULATOR ; CANCER-CELLS ; E-BOX ; PROMOTER ; BINDING ; PROTEINS
英文摘要

Context: Endometriosis is an estrogen-dependent disease. Steroidogenic factor-1 (SF-1), a transcriptional factor essential for activation of multiple steroidogenic genes for estrogen biosynthesis, is undetectable in normal endometrial stromal cells and aberrantly expressed in endometriotic stromal cells.

Objective: The objective of the study was to unravel the mechanism for differential SF-1 expression in endometrial and endometriotic stromal cells.

Design: We identified a CpG island flanking the SF-1 promoter and exon I region and determined its methylation patterns in endometrial and endometriotic cells.

Setting: The study was conducted at Northwestern University.

Patients or Other Participants: Eutopic endometrium from diseasefree subjects (n = 8) and the walls of cystic endometriosis lesions of the ovaries (n = 8) were investigated. Intervention(s): Stromal cells were isolated from these two types of tissues.

Main Outcome Measure(s): Measures are mentioned in Results.

Results: SF-1 mRNA and protein levels in endometriotic stromal cells were significantly higher than those in endometrial stromal cells (P < 0.001). Bisulfite sequencing showed strikingly increased methylation in endometrial cells, compared with endometriotic cells (P < 0.001). Demethylation by 5-aza-2(′)-deoxycytidine increased SF-1 mRNA levels by up to 55.48-fold in endometrial cell (P < 0.05). Luciferase assays showed that the -85/+239 region bearing the CpG island regulated its activity (P < 0.01). Natural or in vitro methylation of this region strikingly reduced SF-1 promoter activity in both cell types (P < 0.01). Chromatin immunoprecipitation assay showed that methyl-CpG-binding domain protein 2 binds to the SF-1 promoter in endometrial but not endometriotic cells.

Conclusions: This is the first demonstration of methylation-dependent regulation of SF-1 in any mammalian tissue. These findings point to a new mechanism for targeting local estrogen biosynthesis in endometriosis.

语种英语
WOS记录号WOS:000248570600067
引用统计
被引频次:104[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62322
专题北京大学第一临床医学院
作者单位1.Northwestern Univ, Feinberg Sch Med, Div Reprod Biol Res, Chicago, IL 60611 USA
2.Northwestern Univ, Feinberg Sch Med, Div Reprod Endocrinol & Infertil, Chicago, IL 60611 USA
3.Northwestern Univ, Feinberg Sch Med, Dept Obstet & Gynecol, Chicago, IL 60611 USA
4.Peking Univ, First Hosp, Dept Obstet & Gynecol, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Xue, Qing,Lin, Zhihong,Yin, Ping,et al. Transcriptional activation of steroidogenic factor-1 by hypomethylation of the 5 &prime; CpG island in endometriosis[J]. JOURNAL OF CLINICAL ENDOCRINOLOGY &amp; METABOLISM,2007,92(8):3261-3267.
APA Xue, Qing.,Lin, Zhihong.,Yin, Ping.,Milad, Magdy P..,Cheng, You-Hong.,...&Bulun, Serdar E..(2007).Transcriptional activation of steroidogenic factor-1 by hypomethylation of the 5 ′ CpG island in endometriosis.JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM,92(8),3261-3267.
MLA Xue, Qing,et al."Transcriptional activation of steroidogenic factor-1 by hypomethylation of the 5 ′ CpG island in endometriosis".JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM 92.8(2007):3261-3267.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Xue, Qing]的文章
[Lin, Zhihong]的文章
[Yin, Ping]的文章
百度学术
百度学术中相似的文章
[Xue, Qing]的文章
[Lin, Zhihong]的文章
[Yin, Ping]的文章
必应学术
必应学术中相似的文章
[Xue, Qing]的文章
[Lin, Zhihong]的文章
[Yin, Ping]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。