北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第三临床医学院  > 眼科  > 期刊论文
学科主题: 临床医学
题名:
Manganese-enhanced MRI optic nerve tracking: effect of intravitreal manganese dose on retinal toxicity
作者: Luo, Lisha1; Xu, Hui2; Li, Ying1; Du, Zhaodong1; Sun, Xiaodong3; Ma, Zhizhong1; Hu, Yuntao1
关键词: manganese ; manganese-enhanced MRI ; optic nerve ; retina ; toxicity ; transmission electron microscopy (TEM)
刊名: NMR IN BIOMEDICINE
发表日期: 2012-12-01
DOI: 10.1002/nbm.2808
卷: 25, 期:12, 页:1360-1368
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biophysics ; Radiology, Nuclear Medicine & Medical Imaging ; Spectroscopy
研究领域[WOS]: Biophysics ; Radiology, Nuclear Medicine & Medical Imaging ; Spectroscopy
关键词[WOS]: IN-VIVO ; VISUAL PATHWAY ; PRIMARY CULTURES ; CONTRAST AGENTS ; CALCIUM INFLUX ; RAT ; NEUROTOXICITY ; TRANSPORT ; CELLS ; MOUSE
英文摘要:

The aim of this study was to provide data on the dose dependence of manganese-enhanced MRI (MEMRI) in the visual pathway of experimental rats and to study the toxicity of MnCl2 to the retina. SpragueDawley rats were intravitreally injected with 2 mu L of 0, 10, 25, 50, 75, 100, 150 and 300 mm MnCl2, respectively. The contrast-to-noise ratio (CNR) of MEMRI for optic nerve enhancement was measured at different concentrations of MnCl2. Simultaneously, the toxicity of manganese was evaluated by counting retinal ganglion cells and by retinal histological examination using light microscopy and transmission electron microscopy. The CNR increased with increasing concentration of MnCl2 up to 75 mm. Retinal ganglion cell densities were reduced significantly when the concentration of MnCl2 in the intravitreal injection was equal to or greater than 75 mm. Increasing numbers of ribosomes in retinal ganglion cells were first detected at 25 mm of MnCl2. The retinal toxicity of MnCl2 at higher concentration also included mitochondrial pathology and cell disruption of retinal ganglion cells, as well as abnormalities of photoreceptor and retinal pigment epithelium cells. It can be concluded that intravitreal injection of MnCl2 induces retinal cell damage that appears to start from 25 mm. The concentration of MnCl2 should not exceed 25 mm through intravitreal injection for visual pathway MEMRI in the rat. Copyright (c) 2012 John Wiley & Sons, Ltd.

语种: 英语
所属项目编号: 2011CB707506 ; YZZ08-9-13 ; 64508-01
项目资助者: National Basic Research Program of China (973) ; Seed Fund from the Peking University Third Hospital ; Linghu Fund from the Peking University Third Hospital
WOS记录号: WOS:000310237600007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62324
Appears in Collections:北京大学第三临床医学院_眼科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Ctr Eye, Hosp 3, Beijing 100191, Peoples R China
2.Peking Univ, Dept Radiol, Hosp 3, Beijing 100191, Peoples R China
3.Shanghai Jiao Tong Univ, Dept Ophthalmol, Affiliated Peoples Hosp 1, Shanghai 200030, Peoples R China

Recommended Citation:
Luo, Lisha,Xu, Hui,Li, Ying,et al. Manganese-enhanced MRI optic nerve tracking: effect of intravitreal manganese dose on retinal toxicity[J]. NMR IN BIOMEDICINE,2012,25(12):1360-1368.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Luo, Lisha]'s Articles
[Xu, Hui]'s Articles
[Li, Ying]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Luo, Lisha]‘s Articles
[Xu, Hui]‘s Articles
[Li, Ying]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace