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Manganese-enhanced MRI optic nerve tracking: effect of intravitreal manganese dose on retinal toxicity
Luo, Lisha1; Xu, Hui2; Li, Ying1; Du, Zhaodong1; Sun, Xiaodong3; Ma, Zhizhong1; Hu, Yuntao1
关键词manganese manganese-enhanced MRI optic nerve retina toxicity transmission electron microscopy (TEM)
刊名NMR IN BIOMEDICINE
2012-12-01
DOI10.1002/nbm.2808
25期:12页:1360-1368
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biophysics ; Radiology, Nuclear Medicine & Medical Imaging ; Spectroscopy
研究领域[WOS]Biophysics ; Radiology, Nuclear Medicine & Medical Imaging ; Spectroscopy
关键词[WOS]IN-VIVO ; VISUAL PATHWAY ; PRIMARY CULTURES ; CONTRAST AGENTS ; CALCIUM INFLUX ; RAT ; NEUROTOXICITY ; TRANSPORT ; CELLS ; MOUSE
英文摘要

The aim of this study was to provide data on the dose dependence of manganese-enhanced MRI (MEMRI) in the visual pathway of experimental rats and to study the toxicity of MnCl2 to the retina. SpragueDawley rats were intravitreally injected with 2 mu L of 0, 10, 25, 50, 75, 100, 150 and 300 mm MnCl2, respectively. The contrast-to-noise ratio (CNR) of MEMRI for optic nerve enhancement was measured at different concentrations of MnCl2. Simultaneously, the toxicity of manganese was evaluated by counting retinal ganglion cells and by retinal histological examination using light microscopy and transmission electron microscopy. The CNR increased with increasing concentration of MnCl2 up to 75 mm. Retinal ganglion cell densities were reduced significantly when the concentration of MnCl2 in the intravitreal injection was equal to or greater than 75 mm. Increasing numbers of ribosomes in retinal ganglion cells were first detected at 25 mm of MnCl2. The retinal toxicity of MnCl2 at higher concentration also included mitochondrial pathology and cell disruption of retinal ganglion cells, as well as abnormalities of photoreceptor and retinal pigment epithelium cells. It can be concluded that intravitreal injection of MnCl2 induces retinal cell damage that appears to start from 25 mm. The concentration of MnCl2 should not exceed 25 mm through intravitreal injection for visual pathway MEMRI in the rat. Copyright (c) 2012 John Wiley & Sons, Ltd.

语种英语
WOS记录号WOS:000310237600007
项目编号2011CB707506 ; YZZ08-9-13 ; 64508-01
资助机构National Basic Research Program of China (973) ; Seed Fund from the Peking University Third Hospital ; Linghu Fund from the Peking University Third Hospital
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62324
专题北京大学第三临床医学院_眼科
北京大学基础医学院
作者单位1.Peking Univ, Ctr Eye, Hosp 3, Beijing 100191, Peoples R China
2.Peking Univ, Dept Radiol, Hosp 3, Beijing 100191, Peoples R China
3.Shanghai Jiao Tong Univ, Dept Ophthalmol, Affiliated Peoples Hosp 1, Shanghai 200030, Peoples R China
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GB/T 7714
Luo, Lisha,Xu, Hui,Li, Ying,et al. Manganese-enhanced MRI optic nerve tracking: effect of intravitreal manganese dose on retinal toxicity[J]. NMR IN BIOMEDICINE,2012,25(12):1360-1368.
APA Luo, Lisha.,Xu, Hui.,Li, Ying.,Du, Zhaodong.,Sun, Xiaodong.,...&Hu, Yuntao.(2012).Manganese-enhanced MRI optic nerve tracking: effect of intravitreal manganese dose on retinal toxicity.NMR IN BIOMEDICINE,25(12),1360-1368.
MLA Luo, Lisha,et al."Manganese-enhanced MRI optic nerve tracking: effect of intravitreal manganese dose on retinal toxicity".NMR IN BIOMEDICINE 25.12(2012):1360-1368.
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