北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第二临床医学院  > 血液科  > 期刊论文
学科主题: 临床医学
题名:
Detecting PML-RAR alpha transcript in acute promyelocytic leukemia using real-time quantitative RT-PCR
作者: Zhu Hong-Hu; Liu Yan-Rong; Qin Ya-Zhen; Bin, Jiang; Shan Fu-Xiang; Wu Shu-Lan; Yang Ping-Di; Re, Zhao; Lu Dao-Pei
关键词: real-time quantitative RT-PCR ; arsenics ; all-trans retinoic acid ; acute promyelocytic leukemia ; PML-RAR alpha
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2007-10-20
卷: 120, 期:20, 页:1803-1808
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: POLYMERASE-CHAIN-REACTION ; RESIDUAL DISEASE DETECTION ; CANCER PROGRAM ; RETINOIC ACID ; FUSION GENE ; DIAGNOSIS ; MULTICENTER ; MANAGEMENT ; REMISSION ; SURVIVAL
英文摘要:

Background Real-time quantitative RT-PCR (RQ-PCR) assay has become a vital tool to monitor residual disease of leukemia. However, the complexity and standardization of RQ-PCR should never be overlooked and the results should be interpreted cautiously in clinical conditions. We aimed to assess the methodology of RQ-PCR and its clinical applications in monitoring molecular kinetics of 36 newly diagnosed cases of acute promyelocytic leukemia patients with t (15; 17) from October 2004 to December 2005.

Methods All the TaqMan probe-based RQ-PCR reactions and analysis were performed on an ABI-PRISM 7500 platform. The quantitation of PML-RAR alpha transcripts was represented by the normalized quotient, that is, PML-RAR alpha transcript copies divided by ABL transcript copies. According to induction therapy, the patients were classed into two groups: group 1 (n=23), three-drug combination including arsenics, all-trans retinoic acid and mitoxantrone; and group 2 (n=13), two-drug combination from all-trans retinoic acid, arsenics and mitoxantrone.

Results The sensitivity of RQ-PCR was 1 per 105 Cells and 5 copies of the PML-RAR alpha transcript could be reproducibly detected. No false positive results occurred in 40 non-acute promyelocytic leukemia samples. Optimal amplification efficiency could be attained, which was determined by the slope of the standard curves (slope: -3.2 - -3.7). The inter-assay and intra-assay variation coefficients of the method were 1.01 % and 0.56% respectively. Although the time to attain hematological complete remission was similar in both groups, the time to achieve molecular remission of group 1 was significantly shorter than that of group 2 (61 days vs; 75 days, P=0.034). The rate of molecular remission within 70 days was higher in group 1 than in group 2 (75.00% vs; 38.46%, P=0.036). Compared with pretreatment, median reduction of the PMIL-RAR alpha transcript before first consolidation therapy differed significantly between group 1 and group 2 (log scale, 3.15 vs 2.31, P=0.024). Interestingly, we found that PML-RAR alpha transcript levels temporarily increased in bone marrow (7 patients) and peripheral blood (22 patients) samples of patients during induction therapy in both groups.

Conclusions The RQ-PCR assay is reliable for the detection of PML-RAR alpha transcripts. Arsenics, all-trans retinoic acid and mitoxantrone triad induction treatment of acute promyelocytic leukemia is superior to two-drug combination induction therapy in terms of the molecular response.

语种: 英语
WOS记录号: WOS:000250539800014
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62336
Appears in Collections:北京大学第二临床医学院_血液科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Peoples Hosp, Inst Hematol, Beijing 100044, Peoples R China
2.Beijing Daopei Hosp, Beijing 100049, Peoples R China

Recommended Citation:
Zhu Hong-Hu,Liu Yan-Rong,Qin Ya-Zhen,et al. Detecting PML-RAR alpha transcript in acute promyelocytic leukemia using real-time quantitative RT-PCR[J]. CHINESE MEDICAL JOURNAL,2007,120(20):1803-1808.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhu Hong-Hu]'s Articles
[Liu Yan-Rong]'s Articles
[Qin Ya-Zhen]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhu Hong-Hu]‘s Articles
[Liu Yan-Rong]‘s Articles
[Qin Ya-Zhen]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace