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学科主题: 临床医学
题名:
Detecting PML-RAR alpha transcript in acute promyelocytic leukemia using real-time quantitative RT-PCR
作者: Zhu Hong-Hu; Liu Yan-Rong; Qin Ya-Zhen; Bin, Jiang; Shan Fu-Xiang; Wu Shu-Lan; Yang Ping-Di; Re, Zhao; Lu Dao-Pei
关键词: real-time quantitative RT-PCR ; arsenics ; all-trans retinoic acid ; acute promyelocytic leukemia ; PML-RAR alpha
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2007-10-20
卷: 120, 期:20, 页:1803-1808
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: POLYMERASE-CHAIN-REACTION ; RESIDUAL DISEASE DETECTION ; CANCER PROGRAM ; RETINOIC ACID ; FUSION GENE ; DIAGNOSIS ; MULTICENTER ; MANAGEMENT ; REMISSION ; SURVIVAL
英文摘要:

Background Real-time quantitative RT-PCR (RQ-PCR) assay has become a vital tool to monitor residual disease of leukemia. However, the complexity and standardization of RQ-PCR should never be overlooked and the results should be interpreted cautiously in clinical conditions. We aimed to assess the methodology of RQ-PCR and its clinical applications in monitoring molecular kinetics of 36 newly diagnosed cases of acute promyelocytic leukemia patients with t (15; 17) from October 2004 to December 2005.

Methods All the TaqMan probe-based RQ-PCR reactions and analysis were performed on an ABI-PRISM 7500 platform. The quantitation of PML-RAR alpha transcripts was represented by the normalized quotient, that is, PML-RAR alpha transcript copies divided by ABL transcript copies. According to induction therapy, the patients were classed into two groups: group 1 (n=23), three-drug combination including arsenics, all-trans retinoic acid and mitoxantrone; and group 2 (n=13), two-drug combination from all-trans retinoic acid, arsenics and mitoxantrone.

Results The sensitivity of RQ-PCR was 1 per 105 Cells and 5 copies of the PML-RAR alpha transcript could be reproducibly detected. No false positive results occurred in 40 non-acute promyelocytic leukemia samples. Optimal amplification efficiency could be attained, which was determined by the slope of the standard curves (slope: -3.2 - -3.7). The inter-assay and intra-assay variation coefficients of the method were 1.01 % and 0.56% respectively. Although the time to attain hematological complete remission was similar in both groups, the time to achieve molecular remission of group 1 was significantly shorter than that of group 2 (61 days vs; 75 days, P=0.034). The rate of molecular remission within 70 days was higher in group 1 than in group 2 (75.00% vs; 38.46%, P=0.036). Compared with pretreatment, median reduction of the PMIL-RAR alpha transcript before first consolidation therapy differed significantly between group 1 and group 2 (log scale, 3.15 vs 2.31, P=0.024). Interestingly, we found that PML-RAR alpha transcript levels temporarily increased in bone marrow (7 patients) and peripheral blood (22 patients) samples of patients during induction therapy in both groups.

Conclusions The RQ-PCR assay is reliable for the detection of PML-RAR alpha transcripts. Arsenics, all-trans retinoic acid and mitoxantrone triad induction treatment of acute promyelocytic leukemia is superior to two-drug combination induction therapy in terms of the molecular response.

语种: 英语
WOS记录号: WOS:000250539800014
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62336
Appears in Collections:北京大学第二临床医学院_血液科_期刊论文

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作者单位: 1.Peking Univ, Peoples Hosp, Inst Hematol, Beijing 100044, Peoples R China
2.Beijing Daopei Hosp, Beijing 100049, Peoples R China

Recommended Citation:
Zhu Hong-Hu,Liu Yan-Rong,Qin Ya-Zhen,et al. Detecting PML-RAR alpha transcript in acute promyelocytic leukemia using real-time quantitative RT-PCR[J]. CHINESE MEDICAL JOURNAL,2007,120(20):1803-1808.
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