IR@PKUHSC  > 北京大学第三临床医学院  > 眼科
学科主题临床医学
The effects of sodium orthovanadate-induced phosphatase inhibition on rat retinal pigment epithelium cell activity
Du, Zhao-Dong1; Hu, Li-Ting2; Hu, Yun-Tao1; Ma, Zhi-Zhong1
刊名CUTANEOUS AND OCULAR TOXICOLOGY
2010-12-01
DOI10.3109/15569527.2010.509851
29期:4页:261-268
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Ophthalmology ; Toxicology
研究领域[WOS]Ophthalmology ; Toxicology
关键词[WOS]CORNEAL ENDOTHELIAL-CELLS ; EPIDERMAL-GROWTH-FACTOR ; TYROSINE PHOSPHORYLATION ; FACTOR RECEPTOR ; EXPRESSION ; BINDING ; TUMOR ; DETACHMENT ; CYCLE ; PTEN
英文摘要

Purpose: The purpose of this study was to assess the contribution of sodium orthovanadate (SOV)-induced phosphatase inhibition to the activation of rat retinal pigment epithelium (RPE) cells.

Methods: Confluent cultures of rat RPE cells were treated with the general phosphatase inhibitor SOV. The effects of SOV on the cell cycle were determined by flow cytometry and protein detection of cyclin A and cyclin D1, two different cell cycle regulatory factors. The effects of SOV on cell differentiation were confirmed by immunostaining for alpha-smooth muscle actin (alpha-SMA). A migration assay was used to evaluate the effects of SOV on cell migration.

Results: SOV could accelerate the cell cycle of RPE cells. Western blotting showed that SOV significantly increased the expression of cyclin A and cyclin D1 in a dose-dependent fashion. The results of alpha-SMA staining and western blotting demonstrated that SOV induced RPE cells to differentiate toward better contractility and motility. The migration assay indicated that SOV improved the migration activity of RPE cells.

Conclusions: Sodium orthovanadate can improve proliferation, differentiation, and migration of rat RPE cells and can also induce the reentry of contact-inhibited rat RPE cells into the cell cycle.

语种英语
WOS记录号WOS:000283688500006
项目编号2007CB512203
资助机构Peking University Third Hospital ; National Basic Research Program of China
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62346
专题北京大学第三临床医学院_眼科
北京大学基础医学院
作者单位1.Peking Univ Third Hosp, Peking Univ Eye Ctr, Beijing 100191, Peoples R China
2.Qingdao Univ Med Coll, Affiliated Hosp, Dept Ophthalmol, Qingdao, Peoples R China
推荐引用方式
GB/T 7714
Du, Zhao-Dong,Hu, Li-Ting,Hu, Yun-Tao,et al. The effects of sodium orthovanadate-induced phosphatase inhibition on rat retinal pigment epithelium cell activity[J]. CUTANEOUS AND OCULAR TOXICOLOGY,2010,29(4):261-268.
APA Du, Zhao-Dong,Hu, Li-Ting,Hu, Yun-Tao,&Ma, Zhi-Zhong.(2010).The effects of sodium orthovanadate-induced phosphatase inhibition on rat retinal pigment epithelium cell activity.CUTANEOUS AND OCULAR TOXICOLOGY,29(4),261-268.
MLA Du, Zhao-Dong,et al."The effects of sodium orthovanadate-induced phosphatase inhibition on rat retinal pigment epithelium cell activity".CUTANEOUS AND OCULAR TOXICOLOGY 29.4(2010):261-268.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Du, Zhao-Dong]的文章
[Hu, Li-Ting]的文章
[Hu, Yun-Tao]的文章
百度学术
百度学术中相似的文章
[Du, Zhao-Dong]的文章
[Hu, Li-Ting]的文章
[Hu, Yun-Tao]的文章
必应学术
必应学术中相似的文章
[Du, Zhao-Dong]的文章
[Hu, Li-Ting]的文章
[Hu, Yun-Tao]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。