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学科主题: 临床医学
题名:
Artificial recombinant cell-penetrating peptides interfere with envelopment of hepatitis B virus nucleocapsid and viral production
作者: Pan, Xiao-Ben; Wei, Lai; Han, Jin-Chao; Ma, Hui; Deng, Kai; Cong, Xu
关键词: Hepatitis B virus ; Nucleocapsid ; Cell penetrating peptide ; Assembly ; Antivirus
刊名: ANTIVIRAL RESEARCH
发表日期: 2011
DOI: 10.1016/j.antiviral.2010.12.001
卷: 89, 期:1, 页:109-114
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pharmacology & Pharmacy ; Virology
研究领域[WOS]: Pharmacology & Pharmacy ; Virology
关键词[WOS]: DRUG-DELIVERY ; CORE ANTIGENS ; PRE-S ; SURFACE ; REPLICATION ; INHIBITION ; PROTEIN ; DNA ; MEMBRANE ; COMPLEX
英文摘要:

Hepatitis B virus (HBV) is a major human infectious pathogen, with over 300 million chronically infected patients worldwide. Current therapeutics for chronic HBV infection have shown only limited success. The plasma membrane represents an impermeable barrier for development of most macromolecular antiviral agents. To develop new anti-HBV macromolecules that can cross the membrane barrier, we designed a series of artificial recombinant peptides including cell penetrating sequence oligoarginine R7 and several nucleocapsid binding subunits (NBS). The anti-HBV function of these peptides was evaluated in a HBV DNA replicative cell line HepG2.2.15. Our results showed that the synthetic recombinant cell penetrating peptides retained the activity of cell penetrating in the living cells. HBV DNA in culture medium markedly decreased in cells treated with cell penetrating peptides bearing NBS for three days. Intracellular HBcAg and HBV DNA replicative intermediates increased by 2-3 fold. In conclusion, the synthetic recombinant cell penetrating peptides bearing NBS can efficiently enter into the cells; block nucleocapsid assembly and inhibit HBV release. Cell penetrating subunit presents a high efficiency tool to deliver synthetic antiviral peptides into cells. (C) 2010 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 30700697 ; 30972605 ; 2008ZX10002-012 ; 2008ZX10002-013
项目资助者: National Natural Science Foundation of China ; Major National S&amp ; T Projects for Infectious Diseases
WOS记录号: WOS:000287065100014
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62351
Appears in Collections:北京大学第二临床医学院_北京大学肝病研究所_期刊论文

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作者单位: Peking Univ, Inst Hepatol, Peoples Hosp, Beijing 100044, Peoples R China

Recommended Citation:
Pan, Xiao-Ben,Wei, Lai,Han, Jin-Chao,et al. Artificial recombinant cell-penetrating peptides interfere with envelopment of hepatitis B virus nucleocapsid and viral production[J]. ANTIVIRAL RESEARCH,2011,89(1):109-114.
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