学科主题临床医学
Artificial recombinant cell-penetrating peptides interfere with envelopment of hepatitis B virus nucleocapsid and viral production
Pan, Xiao-Ben; Wei, Lai; Han, Jin-Chao; Ma, Hui; Deng, Kai; Cong, Xu
关键词Hepatitis B virus Nucleocapsid Cell penetrating peptide Assembly Antivirus
刊名ANTIVIRAL RESEARCH
2011
DOI10.1016/j.antiviral.2010.12.001
89期:1页:109-114
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Pharmacology & Pharmacy ; Virology
研究领域[WOS]Pharmacology & Pharmacy ; Virology
关键词[WOS]DRUG-DELIVERY ; CORE ANTIGENS ; PRE-S ; SURFACE ; REPLICATION ; INHIBITION ; PROTEIN ; DNA ; MEMBRANE ; COMPLEX
英文摘要

Hepatitis B virus (HBV) is a major human infectious pathogen, with over 300 million chronically infected patients worldwide. Current therapeutics for chronic HBV infection have shown only limited success. The plasma membrane represents an impermeable barrier for development of most macromolecular antiviral agents. To develop new anti-HBV macromolecules that can cross the membrane barrier, we designed a series of artificial recombinant peptides including cell penetrating sequence oligoarginine R7 and several nucleocapsid binding subunits (NBS). The anti-HBV function of these peptides was evaluated in a HBV DNA replicative cell line HepG2.2.15. Our results showed that the synthetic recombinant cell penetrating peptides retained the activity of cell penetrating in the living cells. HBV DNA in culture medium markedly decreased in cells treated with cell penetrating peptides bearing NBS for three days. Intracellular HBcAg and HBV DNA replicative intermediates increased by 2-3 fold. In conclusion, the synthetic recombinant cell penetrating peptides bearing NBS can efficiently enter into the cells; block nucleocapsid assembly and inhibit HBV release. Cell penetrating subunit presents a high efficiency tool to deliver synthetic antiviral peptides into cells. (C) 2010 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000287065100014
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62351
专题北京大学第二临床医学院_北京大学肝病研究所
北京大学第二临床医学院_肝病科
作者单位Peking Univ, Inst Hepatol, Peoples Hosp, Beijing 100044, Peoples R China
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GB/T 7714
Pan, Xiao-Ben,Wei, Lai,Han, Jin-Chao,et al. Artificial recombinant cell-penetrating peptides interfere with envelopment of hepatitis B virus nucleocapsid and viral production[J]. ANTIVIRAL RESEARCH,2011,89(1):109-114.
APA Pan, Xiao-Ben,Wei, Lai,Han, Jin-Chao,Ma, Hui,Deng, Kai,&Cong, Xu.(2011).Artificial recombinant cell-penetrating peptides interfere with envelopment of hepatitis B virus nucleocapsid and viral production.ANTIVIRAL RESEARCH,89(1),109-114.
MLA Pan, Xiao-Ben,et al."Artificial recombinant cell-penetrating peptides interfere with envelopment of hepatitis B virus nucleocapsid and viral production".ANTIVIRAL RESEARCH 89.1(2011):109-114.
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