IR@PKUHSC  > 北京大学第二临床医学院
学科主题临床医学
Identification of Potential Serum Biomarkers for Rheumatoid Arthritis by High-Resolution Quantitative Proteomic Analysis
Cheng, Yongjing2; Chen, Yuling3; Sun, Xiaolin1; Li, Yuhui1; Huang, Cibo2; Deng, Haiteng3; Li, Zhanguo1
关键词rheumatoid arthritis proteomic analysis orbitrap ficolin-2
刊名INFLAMMATION
2014-10-01
DOI10.1007/s10753-014-9871-8
37期:5页:1459-1467
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology ; Immunology
资助者National Natural Science Foundation ; National Basic Research Program of China(973 program) ; National Natural Science Foundation ; National Basic Research Program of China(973 program)
研究领域[WOS]Cell Biology ; Immunology
关键词[WOS]SYNOVIAL-FLUID ; CLASSIFICATION CRITERIA ; MASS-SPECTROMETER ; BINDING LECTIN ; H-FICOLIN ; PLASMA ; DISEASE ; PROGRESSION ; DISORDERS ; DIAGNOSIS
英文摘要

The aim of this study was to find serum biomarkers of rheumatoid arthritis (RA) by high-resolution proteomic analysis. Low-abundance proteins from pooling serum sample of early RA patients and healthy controls were enriched using ProteoMiner (TM) enrichment kits. The enriched proteins were separated on SDS-PAGE, digested by trypsin, labeled with tandem mass tag (TMT) reagents, and desalted by C18 stage tip column. Then, the labeled peptides were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) with nano-LC combined with Orbitrap Q Exactive mass spectrometer, and experiments were carried out three times using different specimens, and differentially expressed proteins were screened by intensity ratios of identified peptides. Enzyme-linked immunosorbent assays (ELISAs) were performed to confirm differentially expressed proteins. Twenty-six proteins were found differentially expressed in RA serum by high-resolution proteomic analysis. Among them, levels of thrombospondin-1, ficolin-2, isoform 10 of fibronectin, and apolipoprotein E were higher in RA patients than in healthy controls (RA/healthy control (HC) a parts per thousand yenaEuro parts per thousand 1.5, p < 0.05), while levels of angiotensinogen, paraoxonase/arylesterase 1, isoform E of proteoglycan 4, and plasminogen were significantly lower (RA/HC a parts per thousand currency signaEuro parts per thousand 0.67, p < 0.05). Further study by ELISA showed a higher level of ficolin-2 in the serum of RA patients compared to healthy controls; the level of ficolin-2 was found to be positively correlated with swollen joint counts (SJCs), disease activity score (DAS28), rheumatoid factor, and IgM in RA patients and with DAS28 and IgM in early RA patients through statistical analysis. The results of this study suggest that ficolin-2, as a newly screened biomarker by high-resolution quantitative proteomic analysis, offers the potentiality to become a diagnostic or disease evaluation tool in RA.

语种英语
所属项目编号81030057 ; 31000409 ; 2010CB529100
资助者National Natural Science Foundation ; National Basic Research Program of China(973 program) ; National Natural Science Foundation ; National Basic Research Program of China(973 program)
WOS记录号WOS:000342438200012
Citation statistics
Cited Times:13[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62361
Collection北京大学第二临床医学院
作者单位1.Tsinghua Univ, Sch Life Sci, Beijing 100084, Peoples R China
2.Beijing Hosp, Dept Rheumatol & Immunol, Beijing 100730, Peoples R China
3.Peking Univ, Peoples Hosp, Dept Rheumatol & Immunol, Beijing 100044, Peoples R China
Recommended Citation
GB/T 7714
Cheng, Yongjing,Chen, Yuling,Sun, Xiaolin,et al. Identification of Potential Serum Biomarkers for Rheumatoid Arthritis by High-Resolution Quantitative Proteomic Analysis[J]. INFLAMMATION,2014,37(5):1459-1467.
APA Cheng, Yongjing.,Chen, Yuling.,Sun, Xiaolin.,Li, Yuhui.,Huang, Cibo.,...&Li, Zhanguo.(2014).Identification of Potential Serum Biomarkers for Rheumatoid Arthritis by High-Resolution Quantitative Proteomic Analysis.INFLAMMATION,37(5),1459-1467.
MLA Cheng, Yongjing,et al."Identification of Potential Serum Biomarkers for Rheumatoid Arthritis by High-Resolution Quantitative Proteomic Analysis".INFLAMMATION 37.5(2014):1459-1467.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
谷歌学术
谷歌学术Similar articles in
[Cheng, Yongjing]'s Articles
[Chen, Yuling]'s Articles
[Sun, Xiaolin]'s Articles
百度学术
百度学术Similar articles in
[Cheng, Yongjing]'s Articles
[Chen, Yuling]'s Articles
[Sun, Xiaolin]'s Articles
必应学术
必应学术Similar articles in
[Cheng, Yongjing]'s Articles
[Chen, Yuling]'s Articles
[Sun, Xiaolin]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.