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Identification of Potential Serum Biomarkers for Rheumatoid Arthritis by High-Resolution Quantitative Proteomic Analysis
Cheng, Yongjing2; Chen, Yuling3; Sun, Xiaolin1; Li, Yuhui1; Huang, Cibo2; Deng, Haiteng3; Li, Zhanguo1
关键词rheumatoid arthritis proteomic analysis orbitrap ficolin-2
刊名INFLAMMATION
2014-10-01
DOI10.1007/s10753-014-9871-8
37期:5页:1459-1467
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology ; Immunology
研究领域[WOS]Cell Biology ; Immunology
关键词[WOS]SYNOVIAL-FLUID ; CLASSIFICATION CRITERIA ; MASS-SPECTROMETER ; BINDING LECTIN ; H-FICOLIN ; PLASMA ; DISEASE ; PROGRESSION ; DISORDERS ; DIAGNOSIS
英文摘要

The aim of this study was to find serum biomarkers of rheumatoid arthritis (RA) by high-resolution proteomic analysis. Low-abundance proteins from pooling serum sample of early RA patients and healthy controls were enriched using ProteoMiner (TM) enrichment kits. The enriched proteins were separated on SDS-PAGE, digested by trypsin, labeled with tandem mass tag (TMT) reagents, and desalted by C18 stage tip column. Then, the labeled peptides were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) with nano-LC combined with Orbitrap Q Exactive mass spectrometer, and experiments were carried out three times using different specimens, and differentially expressed proteins were screened by intensity ratios of identified peptides. Enzyme-linked immunosorbent assays (ELISAs) were performed to confirm differentially expressed proteins. Twenty-six proteins were found differentially expressed in RA serum by high-resolution proteomic analysis. Among them, levels of thrombospondin-1, ficolin-2, isoform 10 of fibronectin, and apolipoprotein E were higher in RA patients than in healthy controls (RA/healthy control (HC) a parts per thousand yenaEuro parts per thousand 1.5, p < 0.05), while levels of angiotensinogen, paraoxonase/arylesterase 1, isoform E of proteoglycan 4, and plasminogen were significantly lower (RA/HC a parts per thousand currency signaEuro parts per thousand 0.67, p < 0.05). Further study by ELISA showed a higher level of ficolin-2 in the serum of RA patients compared to healthy controls; the level of ficolin-2 was found to be positively correlated with swollen joint counts (SJCs), disease activity score (DAS28), rheumatoid factor, and IgM in RA patients and with DAS28 and IgM in early RA patients through statistical analysis. The results of this study suggest that ficolin-2, as a newly screened biomarker by high-resolution quantitative proteomic analysis, offers the potentiality to become a diagnostic or disease evaluation tool in RA.

语种英语
WOS记录号WOS:000342438200012
项目编号81030057 ; 31000409 ; 2010CB529100
资助机构National Natural Science Foundation ; National Basic Research Program of China(973 program)
引用统计
被引频次:18[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62361
专题北京大学第二临床医学院
作者单位1.Tsinghua Univ, Sch Life Sci, Beijing 100084, Peoples R China
2.Beijing Hosp, Dept Rheumatol & Immunol, Beijing 100730, Peoples R China
3.Peking Univ, Peoples Hosp, Dept Rheumatol & Immunol, Beijing 100044, Peoples R China
推荐引用方式
GB/T 7714
Cheng, Yongjing,Chen, Yuling,Sun, Xiaolin,et al. Identification of Potential Serum Biomarkers for Rheumatoid Arthritis by High-Resolution Quantitative Proteomic Analysis[J]. INFLAMMATION,2014,37(5):1459-1467.
APA Cheng, Yongjing.,Chen, Yuling.,Sun, Xiaolin.,Li, Yuhui.,Huang, Cibo.,...&Li, Zhanguo.(2014).Identification of Potential Serum Biomarkers for Rheumatoid Arthritis by High-Resolution Quantitative Proteomic Analysis.INFLAMMATION,37(5),1459-1467.
MLA Cheng, Yongjing,et al."Identification of Potential Serum Biomarkers for Rheumatoid Arthritis by High-Resolution Quantitative Proteomic Analysis".INFLAMMATION 37.5(2014):1459-1467.
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