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学科主题: 临床医学
题名:
Identification of Potential Serum Biomarkers for Rheumatoid Arthritis by High-Resolution Quantitative Proteomic Analysis
作者: Cheng, Yongjing2; Chen, Yuling3; Sun, Xiaolin1; Li, Yuhui1; Huang, Cibo2; Deng, Haiteng3; Li, Zhanguo1
关键词: rheumatoid arthritis ; proteomic analysis ; orbitrap ; ficolin-2
刊名: INFLAMMATION
发表日期: 2014-10-01
DOI: 10.1007/s10753-014-9871-8
卷: 37, 期:5, 页:1459-1467
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology ; Immunology
研究领域[WOS]: Cell Biology ; Immunology
关键词[WOS]: SYNOVIAL-FLUID ; CLASSIFICATION CRITERIA ; MASS-SPECTROMETER ; BINDING LECTIN ; H-FICOLIN ; PLASMA ; DISEASE ; PROGRESSION ; DISORDERS ; DIAGNOSIS
英文摘要:

The aim of this study was to find serum biomarkers of rheumatoid arthritis (RA) by high-resolution proteomic analysis. Low-abundance proteins from pooling serum sample of early RA patients and healthy controls were enriched using ProteoMiner (TM) enrichment kits. The enriched proteins were separated on SDS-PAGE, digested by trypsin, labeled with tandem mass tag (TMT) reagents, and desalted by C18 stage tip column. Then, the labeled peptides were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) with nano-LC combined with Orbitrap Q Exactive mass spectrometer, and experiments were carried out three times using different specimens, and differentially expressed proteins were screened by intensity ratios of identified peptides. Enzyme-linked immunosorbent assays (ELISAs) were performed to confirm differentially expressed proteins. Twenty-six proteins were found differentially expressed in RA serum by high-resolution proteomic analysis. Among them, levels of thrombospondin-1, ficolin-2, isoform 10 of fibronectin, and apolipoprotein E were higher in RA patients than in healthy controls (RA/healthy control (HC) a parts per thousand yenaEuro parts per thousand 1.5, p < 0.05), while levels of angiotensinogen, paraoxonase/arylesterase 1, isoform E of proteoglycan 4, and plasminogen were significantly lower (RA/HC a parts per thousand currency signaEuro parts per thousand 0.67, p < 0.05). Further study by ELISA showed a higher level of ficolin-2 in the serum of RA patients compared to healthy controls; the level of ficolin-2 was found to be positively correlated with swollen joint counts (SJCs), disease activity score (DAS28), rheumatoid factor, and IgM in RA patients and with DAS28 and IgM in early RA patients through statistical analysis. The results of this study suggest that ficolin-2, as a newly screened biomarker by high-resolution quantitative proteomic analysis, offers the potentiality to become a diagnostic or disease evaluation tool in RA.

语种: 英语
所属项目编号: 81030057 ; 31000409 ; 2010CB529100
项目资助者: National Natural Science Foundation ; National Basic Research Program of China(973 program)
WOS记录号: WOS:000342438200012
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62361
Appears in Collections:北京大学第二临床医学院_期刊论文

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作者单位: 1.Tsinghua Univ, Sch Life Sci, Beijing 100084, Peoples R China
2.Beijing Hosp, Dept Rheumatol & Immunol, Beijing 100730, Peoples R China
3.Peking Univ, Peoples Hosp, Dept Rheumatol & Immunol, Beijing 100044, Peoples R China

Recommended Citation:
Cheng, Yongjing,Chen, Yuling,Sun, Xiaolin,et al. Identification of Potential Serum Biomarkers for Rheumatoid Arthritis by High-Resolution Quantitative Proteomic Analysis[J]. INFLAMMATION,2014,37(5):1459-1467.
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