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IR@PKUHSC  > 北京大学临床肿瘤学院  > 分子肿瘤学研究室  > 期刊论文
学科主题: 临床医学
题名:
Transfection of PDCD5 Effect on the Biological Behavior of Tumor Cells and Sensitized Gastric Cancer Cells to Cisplatin-Induced Apoptosis
作者: Xu, Hui-Yu1; Chen, Zhi-Wei1; Pan, Yuan-Ming2; Fan, Li1; Guan, Jie1; Lu, You-Yong2
关键词: Gastric cancer ; Programmed cell death 5 (PDCD5) ; Cisplatin ; Apoptosis ; P53
刊名: DIGESTIVE DISEASES AND SCIENCES
发表日期: 2012-07-01
DOI: 10.1007/s10620-012-2090-y
卷: 57, 期:7, 页:1847-1856
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology
研究领域[WOS]: Gastroenterology & Hepatology
关键词[WOS]: HUMAN OVARIAN-CARCINOMA ; PROGNOSTIC-SIGNIFICANCE ; REDUCED EXPRESSION ; DNA-DAMAGE ; IN-VITRO ; DEATH 5 ; GENE ; RESISTANCE ; P53 ; PROTEIN
英文摘要:

Programmed cell death 5 (PDCD5) expression is reduced in various human tumor cells, and the protein concentration and nuclear translocation of PDCD5 is also observed during tumor cell apoptosis.

The purpose of this study was to investigate the differential expression of PDCD5 in six gastric cell lines, and to explore the changes of biological behavior mechanism underlying enhanced apoptosis-inducing effects of cisplatin by PDCD5 over-expression on gastric cancer BGC823 cells.

RT-PCR and real-time PCR were used to determine PDCD5 expression. BGC823/PDCD5 cells were assessed the cellular proliferating ability by MTT assay, soft agar cloning experiments and tumorigenicity in nude mice experiments in vivo. The effects of cisplatin in combination with PDCD5 on the proliferation and apoptosis were measured by MTT, Annexin-V-FITC/PI dual labeling and cell cycle analysis, respectively. Immunofluorescence was used to detect co-localization of p53 and PDCD5 protein to explore the mechanism underlying the synergistic therapeutic effect of PDCD5 with cisplatin (5 mu g/ml for 24 h).

PDCD5 had the highest expression level in the GES1 cell among other cell lines. The growths of BGC823 cells transfected with PDCD5 for six (6th) or 17 (17th) days were both slower than that of BGC823 and BGC823/Neo (P < 0.01). The stable transfection of PDCD5 demonstrated G2/M cell cycle arrest, increased apoptosis and nuclear translocation of PDCD5 and p53 after cisplatin treatment.

Stable transfection of the PDCD5 gene can inhibit the growth of the BGC823 cell line and notably improve apoptosis-inducing effects of cisplatin, indicating a novel strategy for better chemotherapeutic effects on gastric cancer.

语种: 英语
所属项目编号: 39625016 ; 2004CB518708 ; D2007-108 ; D2007-107 ; 11531434
项目资助者: National Natural Science Foundation of China ; State Key Basic Research Program of China ; Heilongjiang Education Department ; Natural Science Foundation of Heilongjiang Province
WOS记录号: WOS:000305746100017
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62388
Appears in Collections:北京大学临床肿瘤学院_分子肿瘤学研究室_期刊论文

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作者单位: 1.Qiqihar Med Univ, Dept Immunol, Qiqihar 161006, Heilongjiang, Peoples R China
2.Peking Univ, Sch Oncol, Beijing Canc Hosp Inst, Mol Oncol Lab, Beijing 100142, Peoples R China

Recommended Citation:
Xu, Hui-Yu,Chen, Zhi-Wei,Pan, Yuan-Ming,et al. Transfection of PDCD5 Effect on the Biological Behavior of Tumor Cells and Sensitized Gastric Cancer Cells to Cisplatin-Induced Apoptosis[J]. DIGESTIVE DISEASES AND SCIENCES,2012,57(7):1847-1856.
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