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学科主题: 公共卫生
题名:
The over-expression of p53 H179Y residue mutation causes the increase of cyclin A1 and Cdk4 expression in HELF cells
作者: Yang, Di; Qi, Yitao; Chen, Qian; Wang, Zhiqin; Jin, Xi; Gao, Jie; Fu, Juanling; Xiao, Xilong; Zhou, Zongcan
关键词: p53 mutation ; cell cycle regulation ; cell size ; cyclin A1 ; Cdk4
刊名: MOLECULAR AND CELLULAR BIOCHEMISTRY
发表日期: 2007-10-01
DOI: 10.1007/s11010-007-9503-9
卷: 304, 期:1-2, 页:219-226
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology
研究领域[WOS]: Cell Biology
关键词[WOS]: MUTANT P53 ; WILD-TYPE ; GENE-EXPRESSION ; PROTEINS BIND ; CANCER CELLS ; TUMOR-CELLS ; DNA ; TRANSCRIPTION ; RNA ; MODULATION
英文摘要:

Down-regulation of p53 expression has been found in a broad range of human cancers and cell proliferation disorders, indicating that p53 plays a key role in cell cycle regulation and tumor suppression. In our current study, we transfected human embryonic lung fibroblast (HELF) cells with pcDNA3-wild-type p53 (pcDNA3-wtp53) plasmid, or pcDNA3-H179Y-mutated p53 (pcDNA3-mtp53) plasmid that mimics the mutation found in some human lung tumors, and further studied the role of p53 in the regulation of cell proliferation. Over expression of wild-type p53 caused cell cycle arrest at G1 phase with reduced cell size, decreased expression of cyclin D3, cyclin E, Cdk2 and Cdk4, and increased expression of p21. In contrast, over expression of H179Y-mutant p53 promoted G1 to S phase transition with enlarged cell size and increased cyclin A1 and Cdk4 expression in HELF cells. These results indicate that mutation at the p53 H179Y residue up-regulates cyclin A1 and Cdk4 expression, and promotes HELF cell proliferation.

语种: 英语
WOS记录号: WOS:000249629000024
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62389
Appears in Collections:北京大学公共卫生学院_毒理学系_期刊论文

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作者单位: 1.China Agr Univ, Coll Vet Med, Dept Pharmacol & Toxicol, Beijing 100094, Peoples R China
2.Peking Univ, Ctr Hlth Sci, Dept Toxicol, Beijing 100083, Peoples R China

Recommended Citation:
Yang, Di,Qi, Yitao,Chen, Qian,et al. The over-expression of p53 H179Y residue mutation causes the increase of cyclin A1 and Cdk4 expression in HELF cells[J]. MOLECULAR AND CELLULAR BIOCHEMISTRY,2007,304(1-2):219-226.
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