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The over-expression of p53 H179Y residue mutation causes the increase of cyclin A1 and Cdk4 expression in HELF cells
Yang, Di; Qi, Yitao; Chen, Qian; Wang, Zhiqin; Jin, Xi; Gao, Jie; Fu, Juanling; Xiao, Xilong; Zhou, Zongcan
关键词p53 mutation cell cycle regulation cell size cyclin A1 Cdk4
刊名MOLECULAR AND CELLULAR BIOCHEMISTRY
2007-10-01
DOI10.1007/s11010-007-9503-9
304期:1-2页:219-226
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology
研究领域[WOS]Cell Biology
关键词[WOS]MUTANT P53 ; WILD-TYPE ; GENE-EXPRESSION ; PROTEINS BIND ; CANCER CELLS ; TUMOR-CELLS ; DNA ; TRANSCRIPTION ; RNA ; MODULATION
英文摘要

Down-regulation of p53 expression has been found in a broad range of human cancers and cell proliferation disorders, indicating that p53 plays a key role in cell cycle regulation and tumor suppression. In our current study, we transfected human embryonic lung fibroblast (HELF) cells with pcDNA3-wild-type p53 (pcDNA3-wtp53) plasmid, or pcDNA3-H179Y-mutated p53 (pcDNA3-mtp53) plasmid that mimics the mutation found in some human lung tumors, and further studied the role of p53 in the regulation of cell proliferation. Over expression of wild-type p53 caused cell cycle arrest at G1 phase with reduced cell size, decreased expression of cyclin D3, cyclin E, Cdk2 and Cdk4, and increased expression of p21. In contrast, over expression of H179Y-mutant p53 promoted G1 to S phase transition with enlarged cell size and increased cyclin A1 and Cdk4 expression in HELF cells. These results indicate that mutation at the p53 H179Y residue up-regulates cyclin A1 and Cdk4 expression, and promotes HELF cell proliferation.

语种英语
WOS记录号WOS:000249629000024
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62389
Collection北京大学公共卫生学院_毒理学系
作者单位1.China Agr Univ, Coll Vet Med, Dept Pharmacol & Toxicol, Beijing 100094, Peoples R China
2.Peking Univ, Ctr Hlth Sci, Dept Toxicol, Beijing 100083, Peoples R China
Recommended Citation
GB/T 7714
Yang, Di,Qi, Yitao,Chen, Qian,et al. The over-expression of p53 H179Y residue mutation causes the increase of cyclin A1 and Cdk4 expression in HELF cells[J]. MOLECULAR AND CELLULAR BIOCHEMISTRY,2007,304(1-2):219-226.
APA Yang, Di.,Qi, Yitao.,Chen, Qian.,Wang, Zhiqin.,Jin, Xi.,...&Zhou, Zongcan.(2007).The over-expression of p53 H179Y residue mutation causes the increase of cyclin A1 and Cdk4 expression in HELF cells.MOLECULAR AND CELLULAR BIOCHEMISTRY,304(1-2),219-226.
MLA Yang, Di,et al."The over-expression of p53 H179Y residue mutation causes the increase of cyclin A1 and Cdk4 expression in HELF cells".MOLECULAR AND CELLULAR BIOCHEMISTRY 304.1-2(2007):219-226.
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