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学科主题: 临床医学
题名:
Icariside II ameliorates diabetic nephropathy in streptozotocin-induced diabetic rats
作者: Tian, Wenjie1,2; Lei, Hongen1; Guan, Ruili1; Xu, Yongde1; Li, Huixi1; Wang, Lin1; Yang, Bicheng1; Gao, Zhezhu1; Xin, Zhongcheng1
关键词: diabetic nephropathy ; icariside II ; EdU ; diabetes mellitus ; label retaining progenitor cells
刊名: DRUG DESIGN DEVELOPMENT AND THERAPY
发表日期: 2015
DOI: 10.2147/DDDT.S90060
卷: 9, 页:5147-5157
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Medicinal ; Pharmacology & Pharmacy
研究领域[WOS]: Pharmacology & Pharmacy
关键词[WOS]: MESENCHYMAL STEM-CELLS ; ENDOTHELIAL-CELLS ; IV COLLAGEN ; EXPRESSION ; ICARIIN ; GROWTH ; BETA ; DIFFERENTIATION ; PROLIFERATION ; DYSFUNCTION
英文摘要:

Purpose: To investigate the therapeutic effects and potential mechanisms of icariside II (ICA II) on reversing diabetic nephropathy in streptozotocin (STZ)-induced type I diabetic rats.

Methods: Newborn male Sprague Dawley rats were labeled with thymidine analog 5-ethynyl-2-deoxyuridine (EdU) for tracking endogenous label retaining progenitor cells (LRCs). At age of 8 weeks, 48 rats were randomly divided into three groups: normal control group (n=16), diabetes mellitus group (DM; n=16), and diabetes mellitus plus ICA II therapy group (DM+ICA II, n=16). Eight weeks induced for diabetes with STZ, rats in DM group and DM+ ICA II group were treated with vehicle or ICA II (5 mg/kg/day) for another 8 weeks, respectively. Then, blood creatinine, 24-hour urine protein, blood urea nitrogen, and glycosylated hemoglobin were measured, as well as the expression of von Willebrand factor, malondialdehyde, transforming growth factor-beta/drosophila mothers against decapentaplegic protein/connective tissue growth factor (TGF-beta/Smad/CTGF) signaling, marker of proliferation Ki-67, and EdU+LRCs in renal tissues.

Results: Increased levels of creatinine, 24-hour urine protein, and blood urea nitrogen and remarkably decreased proportion of normal glomeruli and increased proportions of I, IIa, IIb, and III glomeruli were observed in diabetic rats, while ICA II could reverse these changes. Interestingly, ICA II could significantly downregulate the levels of malondialdehyde and TGF-beta/Smad/CTGF signaling and increase the expression of von Willebrand factor, Ki-67, and EdU+ LRCs in the kidney.

Conclusion: ICA II treatment could ameliorate diabetic nephropathy in STZ-induced diabetic rats by increasing endothelial cell contents, downregulating TGF-beta/Smad/CTGF signaling pathway and oxidative stress level, and promoting cell proliferation both in kidney cortex and medulla. These beneficial effects appear to be mediated by its antioxidant capacity and recruitment of endogenous EdU+ progenitor cells into the kidney tissue.

语种: 英语
所属项目编号: 81270693 ; 81272531 ; 81470921
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000361015300002
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62418
Appears in Collections:北京大学第一临床医学院_男科中心_期刊论文

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作者单位: 1.Jilin Univ, Dept Urol, Hosp 2, Changchun 130023, Peoples R China
2.Peking Univ, Androl Ctr, Hosp 1, Beijing 100034, Peoples R China

Recommended Citation:
Tian, Wenjie,Lei, Hongen,Guan, Ruili,et al. Icariside II ameliorates diabetic nephropathy in streptozotocin-induced diabetic rats[J]. DRUG DESIGN DEVELOPMENT AND THERAPY,2015,9:5147-5157.
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