IR@PKUHSC  > 北京大学基础医学院  > 病理学系
学科主题基础医学
STR DNA genotyping of hydatidiform moles in South China
Zheng, Xing-Zheng1; Hui, Pei2; Chang, Bin3; Gao, Zhi-Bin4; Li, Yan1; Wu, Bing-Quan1; Zhang, Bo1
关键词Short-tandem-repeat (STR) DNA genotyping diagnosis hydatidiform mole non-molar gestation
刊名INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY
2014
7期:8页:4704-4719
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology ; Pathology
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
研究领域[WOS]Oncology ; Pathology
关键词[WOS]GESTATIONAL TROPHOBLASTIC DISEASE ; IMPROVE-MORPHOLOGIC-DIAGNOSIS ; IN-SITU HYBRIDIZATION ; ANCILLARY-TECHNIQUES ; P57 IMMUNOHISTOCHEMISTRY ; DIFFERENTIAL-DIAGNOSIS ; SPONTANEOUS-ABORTIONS ; MOLAR PREGNANCY ; FLOW-CYTOMETRY ; ORIGIN
英文摘要

Objective: To evacuate whether short-tandem-repeat (STR) DNA genotyping is effective for diagnostic measure to precisely classify hydatidiform moles. Methods: 150 cases were selected based on histologic features that were previously diagnosed or suspected molar pregnancy. All sections were stained with hematoxylin as a quality control method, and guided the microscopic dissection. DNA was extracted from dissected chorionic villi and paired maternal endometrial FFPE tissue sections. Then, STR DNA genotyping was performed by AmpFlSTR (R) Sinofiler (TM) PCR Amplification system (Applied Biosystems, Inc). Data collection and analysis were carried out using GeneMapper (R) ID-X version 1.2 (Applied Biosystems, Inc). Results: DNA genotyping was informative in all cases, leading to identification of 129 cases with abnormal genotype, including 95 complete and 34 partial moles, except 4 cases failed in PCR. Among 95 complete moles, 92 cases were monospermic and three were dispermic. Among 34 partial moles, 32 were dispermic and 2 were monospermic. The remaining 17 cases were balanced biallelic gestations. Conclusion: STR DNA genotyping is effective for diagnostic measure to precisely classify hydatidiform moles. And in the absence of laser capture microdissection (LCM), hematoxylin staining plus manual dissection under microscopic guided is a more economic and practical method.

语种英语
所属项目编号81260104
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
WOS记录号WOS:000345120900014
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文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62420
Collection北京大学基础医学院_病理学系
作者单位1.Yale Univ, Sch Med, Dept Pathol, New Haven, CT 06510 USA
2.Yurao Peoples Hosp, Dept Pathol, Hangzhou, Zhejiang, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Pathol, Beijing 100871, Peoples R China
4.Fudan Univ, Shanghai Canc Ctr, Dept Pathol, Shanghai 200433, Peoples R China
Recommended Citation
GB/T 7714
Zheng, Xing-Zheng,Hui, Pei,Chang, Bin,et al. STR DNA genotyping of hydatidiform moles in South China[J]. INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY,2014,7(8):4704-4719.
APA Zheng, Xing-Zheng.,Hui, Pei.,Chang, Bin.,Gao, Zhi-Bin.,Li, Yan.,...&Zhang, Bo.(2014).STR DNA genotyping of hydatidiform moles in South China.INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY,7(8),4704-4719.
MLA Zheng, Xing-Zheng,et al."STR DNA genotyping of hydatidiform moles in South China".INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY 7.8(2014):4704-4719.
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