IR@PKUHSC  > 基础医学院  > 病理学系
学科主题基础医学
STR DNA genotyping of hydatidiform moles in South China
Zheng, Xing-Zheng1; Hui, Pei2; Chang, Bin3; Gao, Zhi-Bin4; Li, Yan1; Wu, Bing-Quan1; Zhang, Bo1
关键词Short-tandem-repeat (STR) DNA genotyping diagnosis hydatidiform mole non-molar gestation
刊名INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY
2014
7期:8页:4704-4719
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology ; Pathology
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
研究领域[WOS]Oncology ; Pathology
关键词[WOS]GESTATIONAL TROPHOBLASTIC DISEASE ; IMPROVE-MORPHOLOGIC-DIAGNOSIS ; IN-SITU HYBRIDIZATION ; ANCILLARY-TECHNIQUES ; P57 IMMUNOHISTOCHEMISTRY ; DIFFERENTIAL-DIAGNOSIS ; SPONTANEOUS-ABORTIONS ; MOLAR PREGNANCY ; FLOW-CYTOMETRY ; ORIGIN
英文摘要

Objective: To evacuate whether short-tandem-repeat (STR) DNA genotyping is effective for diagnostic measure to precisely classify hydatidiform moles. Methods: 150 cases were selected based on histologic features that were previously diagnosed or suspected molar pregnancy. All sections were stained with hematoxylin as a quality control method, and guided the microscopic dissection. DNA was extracted from dissected chorionic villi and paired maternal endometrial FFPE tissue sections. Then, STR DNA genotyping was performed by AmpFlSTR (R) Sinofiler (TM) PCR Amplification system (Applied Biosystems, Inc). Data collection and analysis were carried out using GeneMapper (R) ID-X version 1.2 (Applied Biosystems, Inc). Results: DNA genotyping was informative in all cases, leading to identification of 129 cases with abnormal genotype, including 95 complete and 34 partial moles, except 4 cases failed in PCR. Among 95 complete moles, 92 cases were monospermic and three were dispermic. Among 34 partial moles, 32 were dispermic and 2 were monospermic. The remaining 17 cases were balanced biallelic gestations. Conclusion: STR DNA genotyping is effective for diagnostic measure to precisely classify hydatidiform moles. And in the absence of laser capture microdissection (LCM), hematoxylin staining plus manual dissection under microscopic guided is a more economic and practical method.

语种英语
所属项目编号81260104
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
WOS记录号WOS:000345120900014
引用统计
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62420
专题基础医学院_病理学系
作者单位1.Yale Univ, Sch Med, Dept Pathol, New Haven, CT 06510 USA
2.Yurao Peoples Hosp, Dept Pathol, Hangzhou, Zhejiang, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Pathol, Beijing 100871, Peoples R China
4.Fudan Univ, Shanghai Canc Ctr, Dept Pathol, Shanghai 200433, Peoples R China
推荐引用方式
GB/T 7714
Zheng, Xing-Zheng,Hui, Pei,Chang, Bin,et al. STR DNA genotyping of hydatidiform moles in South China[J]. INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY,2014,7(8):4704-4719.
APA Zheng, Xing-Zheng.,Hui, Pei.,Chang, Bin.,Gao, Zhi-Bin.,Li, Yan.,...&Zhang, Bo.(2014).STR DNA genotyping of hydatidiform moles in South China.INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY,7(8),4704-4719.
MLA Zheng, Xing-Zheng,et al."STR DNA genotyping of hydatidiform moles in South China".INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY 7.8(2014):4704-4719.
条目包含的文件 下载所有文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
STR DNA genotyping o(1008KB)期刊论文出版稿开放获取CC BY-NC-SA浏览 下载
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zheng, Xing-Zheng]的文章
[Hui, Pei]的文章
[Chang, Bin]的文章
百度学术
百度学术中相似的文章
[Zheng, Xing-Zheng]的文章
[Hui, Pei]的文章
[Chang, Bin]的文章
必应学术
必应学术中相似的文章
[Zheng, Xing-Zheng]的文章
[Hui, Pei]的文章
[Chang, Bin]的文章
相关权益政策
暂无数据
收藏/分享
文件名: STR DNA genotyping of hydatidiform moles in South China.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。