IR@PKUHSC  > 北京大学药学院  > 天然药物学系
学科主题药学
Effect of stability of internal standard on quantification of 15 flavonoids in Epimedium using CZE
Chen, Xiao-jia1; Tu, Peng-fei2; Jiang, Yong2; Wang, Yi-tao1; Li, Shao-Ping1
关键词CZE Epimedium Flavonoids Internal standard Stability
刊名JOURNAL OF SEPARATION SCIENCE
2009
32期:2页:275-281
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Analytical
资助者Macao Science and Technology Development Fund ; Macao Science and Technology Development Fund
研究领域[WOS]Chemistry
关键词[WOS]PERFORMANCE LIQUID-CHROMATOGRAPHY ; ELECTROKINETIC CAPILLARY CHROMATOGRAPHY ; DIODE-ARRAY DETECTION ; ZONE-ELECTROPHORESIS ; MASS-SPECTROMETRY ; BREVICORNUM-MAXIM ; HERBA-EPIMEDII ; GLYCOSIDES ; KOREANUM ; EXTRACTION
英文摘要

A CZE method was developed for the simultaneous determination of 15 flavonoids, including epimedin B, epimedin A, hexandraside F, epimedin C, icariin, sagittatoside B, sagittatoside A, hexandraside E, 2 ′′-O-rhamnosyl icariside II, baohuoside VII, baohuoside I, caohuoside C, epimedoside C, baohuoside II, and kaerripferol-3-O-rhamnoside, in different species of Epimedium, and the effect of stability of internal standard (IS) on quantification was also investigated. As a result, rutin was not available. for use as an IS because of its unstable property in sample solution, which suggested that the stability of IS both in standards and sample solution should be considered for the analysis. Using stable daidzein as IS, the analysis was performed within 35 min by using 50 mM borax buffer containing 20% ACN as a modifier (pH 10.0), while separation voltage was 25 kV and temperature was at 30 C. The method was validated to be accurate, simple, and repeatable, and was successfully applied to the analysis of 36 samples from 17 species of Epimedium.

语种英语
所属项目编号049/2005/A-R1
资助者Macao Science and Technology Development Fund ; Macao Science and Technology Development Fund
WOS记录号WOS:000263304500016
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62432
专题北京大学药学院_天然药物学系
作者单位1.Univ Macau, Inst Chinese Med Sci, Macao, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, Dept Nat Med, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Chen, Xiao-jia,Tu, Peng-fei,Jiang, Yong,et al. Effect of stability of internal standard on quantification of 15 flavonoids in Epimedium using CZE[J]. JOURNAL OF SEPARATION SCIENCE,2009,32(2):275-281.
APA Chen, Xiao-jia,Tu, Peng-fei,Jiang, Yong,Wang, Yi-tao,&Li, Shao-Ping.(2009).Effect of stability of internal standard on quantification of 15 flavonoids in Epimedium using CZE.JOURNAL OF SEPARATION SCIENCE,32(2),275-281.
MLA Chen, Xiao-jia,et al."Effect of stability of internal standard on quantification of 15 flavonoids in Epimedium using CZE".JOURNAL OF SEPARATION SCIENCE 32.2(2009):275-281.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Chen, Xiao-jia]的文章
[Tu, Peng-fei]的文章
[Jiang, Yong]的文章
百度学术
百度学术中相似的文章
[Chen, Xiao-jia]的文章
[Tu, Peng-fei]的文章
[Jiang, Yong]的文章
必应学术
必应学术中相似的文章
[Chen, Xiao-jia]的文章
[Tu, Peng-fei]的文章
[Jiang, Yong]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。