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学科主题: 药学
题名:
Purification, antioxidant and immunological activities of polysaccharides from Actinidia Chinensis roots
作者: Zhang, Lin1,2; Zhang, Wuxia1; Wang, Qingjie1; Wang, Dongdong1; Dong, Dongqi1; Mu, Haibo1; Ye, Xin-Shan2; Duan, Jinyou1
关键词: Actinidia Chinensis ; Polysaccharides ; Purification ; Antioxidant activity ; lmmunomodulation
刊名: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
发表日期: 2015
DOI: 10.1016/j.ijbiomac.2014.09.056
卷: 72, 页:975-983
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: GEL-PERMEATION CHROMATOGRAPHY ; WATER-SOLUBLE POLYSACCHARIDE ; PALIURUS BATAL. ILJINSKAJA ; BIOLOGICAL-ACTIVITIES ; STRUCTURAL FEATURES ; ASSISTED EXTRACTION ; ANTITUMOR-ACTIVITY ; FRUCTUS-JUJUBAE ; PC12 CELLS ; OPTIMIZATION
英文摘要:

Two water-soluble polysaccharides (ACPS1 and ACPS2) were isolated from the roots of Actinidia Chinonsis by DEAE-52 cellulose and Sephacryl S300 chromatography. Preliminary structural characterization was conducted by physicochemical property, Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM) analysis. ACPS1, with an average molecular weight of 5.58 x 10(6) Da, was mainly composed of rhamnose, arabinose, xylose, mannose and galactose in an approximate molar ratio of 1.48:4.28:4.30:1.00:17.83. ACPS2, with a high average molecular weight of 1.23 x 10(6) Da, mainly contained rhamnose, arabinose and galactose in a molar ratio of approximately 1.00:2.33:6.61. Both ACPS1 and ACPS2 exhibited the remarkable antioxidant activity to scavenge the DPPH radical and significant protective effects on H2O2-induced HEK 293 cells death in a concentration-dependent manner. Meanwhile, in vitro immunomodulatory activities of the two polysaccharides were evaluated. The results showed that treatment with 50-300 mu g/mL of the samples could increase NO production and phagocytic activity of macrophages in a dose-dependent manner. The present results suggested that the two polysaccharides from Actinidia Chinensis may be potential antioxidant and immunomodulatory agents for preparing functional foods and nutraceuticals applied in food and pharmaceutical industries. (C) 2014 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 31270860 ; NCET-13-0480 ; K20120216
项目资助者: National Natural Science Foundation of China (NSFC) ; Program for New Century Excellent Talents in University ; State Key Laboratory of Natural and Biomimetic Drugs, peking University ; &prime ; Interdisciplinary Cooperation Team&prime ; Program for Science and Technology Innovation of the Chinese Academy of Science
WOS记录号: WOS:000347577900128
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62433
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Northwest A&F Univ, Coll Sci, Yangling 712100, Shaanxi, Peoples R China
2.Peking Univ, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci, Beijing 100191, Peoples R China

Recommended Citation:
Zhang, Lin,Zhang, Wuxia,Wang, Qingjie,et al. Purification, antioxidant and immunological activities of polysaccharides from Actinidia Chinensis roots[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2015,72:975-983.
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