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Purification, antioxidant and immunological activities of polysaccharides from Actinidia Chinensis roots
Zhang, Lin1,2; Zhang, Wuxia1; Wang, Qingjie1; Wang, Dongdong1; Dong, Dongqi1; Mu, Haibo1; Ye, Xin-Shan2; Duan, Jinyou1
关键词Actinidia Chinensis Polysaccharides Purification Antioxidant activity lmmunomodulation
刊名INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
2015
DOI10.1016/j.ijbiomac.2014.09.056
72页:975-983
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology
资助者National Natural Science Foundation of China (NSFC) ; Program for New Century Excellent Talents in University ; State Key Laboratory of Natural and Biomimetic Drugs, peking University ; &prime ; Interdisciplinary Cooperation Team&prime ; Program for Science and Technology Innovation of the Chinese Academy of Science ; National Natural Science Foundation of China (NSFC) ; Program for New Century Excellent Talents in University ; State Key Laboratory of Natural and Biomimetic Drugs, peking University ; &prime ; Interdisciplinary Cooperation Team&prime ; Program for Science and Technology Innovation of the Chinese Academy of Science
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]GEL-PERMEATION CHROMATOGRAPHY ; WATER-SOLUBLE POLYSACCHARIDE ; PALIURUS BATAL. ILJINSKAJA ; BIOLOGICAL-ACTIVITIES ; STRUCTURAL FEATURES ; ASSISTED EXTRACTION ; ANTITUMOR-ACTIVITY ; FRUCTUS-JUJUBAE ; PC12 CELLS ; OPTIMIZATION
英文摘要

Two water-soluble polysaccharides (ACPS1 and ACPS2) were isolated from the roots of Actinidia Chinonsis by DEAE-52 cellulose and Sephacryl S300 chromatography. Preliminary structural characterization was conducted by physicochemical property, Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM) analysis. ACPS1, with an average molecular weight of 5.58 x 10(6) Da, was mainly composed of rhamnose, arabinose, xylose, mannose and galactose in an approximate molar ratio of 1.48:4.28:4.30:1.00:17.83. ACPS2, with a high average molecular weight of 1.23 x 10(6) Da, mainly contained rhamnose, arabinose and galactose in a molar ratio of approximately 1.00:2.33:6.61. Both ACPS1 and ACPS2 exhibited the remarkable antioxidant activity to scavenge the DPPH radical and significant protective effects on H2O2-induced HEK 293 cells death in a concentration-dependent manner. Meanwhile, in vitro immunomodulatory activities of the two polysaccharides were evaluated. The results showed that treatment with 50-300 mu g/mL of the samples could increase NO production and phagocytic activity of macrophages in a dose-dependent manner. The present results suggested that the two polysaccharides from Actinidia Chinensis may be potential antioxidant and immunomodulatory agents for preparing functional foods and nutraceuticals applied in food and pharmaceutical industries. (C) 2014 Elsevier B.V. All rights reserved.

语种英语
所属项目编号31270860 ; NCET-13-0480 ; K20120216
资助者National Natural Science Foundation of China (NSFC) ; Program for New Century Excellent Talents in University ; State Key Laboratory of Natural and Biomimetic Drugs, peking University ; &prime ; Interdisciplinary Cooperation Team&prime ; Program for Science and Technology Innovation of the Chinese Academy of Science ; National Natural Science Foundation of China (NSFC) ; Program for New Century Excellent Talents in University ; State Key Laboratory of Natural and Biomimetic Drugs, peking University ; &prime ; Interdisciplinary Cooperation Team&prime ; Program for Science and Technology Innovation of the Chinese Academy of Science
WOS记录号WOS:000347577900128
引用统计
被引频次:16[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62433
专题北京大学药学院
作者单位1.Northwest A&F Univ, Coll Sci, Yangling 712100, Shaanxi, Peoples R China
2.Peking Univ, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Lin,Zhang, Wuxia,Wang, Qingjie,et al. Purification, antioxidant and immunological activities of polysaccharides from Actinidia Chinensis roots[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2015,72:975-983.
APA Zhang, Lin.,Zhang, Wuxia.,Wang, Qingjie.,Wang, Dongdong.,Dong, Dongqi.,...&Duan, Jinyou.(2015).Purification, antioxidant and immunological activities of polysaccharides from Actinidia Chinensis roots.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,72,975-983.
MLA Zhang, Lin,et al."Purification, antioxidant and immunological activities of polysaccharides from Actinidia Chinensis roots".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 72(2015):975-983.
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