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学科主题基础医学
Qa-2 associated lipid rafts are indispensable in the final maturation of CD4(+)CD8(-) thymocytes
Li, Juan1; Li, Hai-Dong2; Zhang, Yu3; Zhang, Jun3
关键词Thymocyte Qa-2 Lipid rafts CD4SP SP4
刊名IMMUNOLOGY LETTERS
2012-12-01
DOI10.1016/j.imlet.2012.10.006
148期:2页:163-171
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Immunology
研究领域[WOS]Immunology
关键词[WOS]T-CELLS ; MEMBRANE RAFTS ; ACTIVATION ; RECEPTOR ; PROTEINS ; DYNAMICS ; LCK ; CD4 ; FYN
英文摘要

Lipid rafts have been shown to play significant roles in thymocyte development. However, the exact role of lipid rafts in single positive (SP) thymocyte differentiation is poorly characterized. We previously defined a developmental program (SP1 -> SP4) for CD4SP thymocytes. In this study, we found that lipid raft components were up-regulated during CD4SP maturation. Qa-2, a unique marker for the most mature SP4 subset, was localized to lipid rafts and heterogeneously expressed in SP4 cells. Functional assays showed that the proliferation capacity of SP4 cells correlated with the expression of Qa-2. Raft-disruption on both CD4SP and epithelial cells by cholesterol extraction or cholesterol oxidation in a medullary thymic epithelial cell (mTEC)-supported co-culture system impaired the transition from SP3 to SP4. This result was further confirmed in fetal thymic organ culture system. Collectively, these studies suggest that raft-associated signaling between mTECs and thymocytes drives the differentiation of CD4SP thymocytes and lipid rafts are involved in the final maturation of SP4 thymocytes. (C) 2012 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000314192500010
项目编号30900735 ; 81070271 ; 211012 ; 2011CB946103
资助机构National Natural Science Foundation of China ; Key Project of Chinese Ministry of Education ; National Basic Research Program of China
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62442
专题北京大学基础医学院_免疫学系
作者单位1.Tianjin Med Univ, Dept Immunol, Tianjin Key Lab Cellular & Mol Immunol, Key Lab,Educ Minist, Tianjin 300070, Peoples R China
2.Tianjin Med Univ, Dept Biochem, Tianjin, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Sch Basic Sci, Dept Immunol, Beijing 100871, Peoples R China
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GB/T 7714
Li, Juan,Li, Hai-Dong,Zhang, Yu,et al. Qa-2 associated lipid rafts are indispensable in the final maturation of CD4(+)CD8(-) thymocytes[J]. IMMUNOLOGY LETTERS,2012,148(2):163-171.
APA Li, Juan,Li, Hai-Dong,Zhang, Yu,&Zhang, Jun.(2012).Qa-2 associated lipid rafts are indispensable in the final maturation of CD4(+)CD8(-) thymocytes.IMMUNOLOGY LETTERS,148(2),163-171.
MLA Li, Juan,et al."Qa-2 associated lipid rafts are indispensable in the final maturation of CD4(+)CD8(-) thymocytes".IMMUNOLOGY LETTERS 148.2(2012):163-171.
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