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学科主题: 基础医学
题名:
Qa-2 associated lipid rafts are indispensable in the final maturation of CD4(+)CD8(-) thymocytes
作者: Li, Juan1; Li, Hai-Dong2; Zhang, Yu3; Zhang, Jun3
关键词: Thymocyte ; Qa-2 ; Lipid rafts ; CD4SP ; SP4
刊名: IMMUNOLOGY LETTERS
发表日期: 2012-12-01
DOI: 10.1016/j.imlet.2012.10.006
卷: 148, 期:2, 页:163-171
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Immunology
研究领域[WOS]: Immunology
关键词[WOS]: T-CELLS ; MEMBRANE RAFTS ; ACTIVATION ; RECEPTOR ; PROTEINS ; DYNAMICS ; LCK ; CD4 ; FYN
英文摘要:

Lipid rafts have been shown to play significant roles in thymocyte development. However, the exact role of lipid rafts in single positive (SP) thymocyte differentiation is poorly characterized. We previously defined a developmental program (SP1 -> SP4) for CD4SP thymocytes. In this study, we found that lipid raft components were up-regulated during CD4SP maturation. Qa-2, a unique marker for the most mature SP4 subset, was localized to lipid rafts and heterogeneously expressed in SP4 cells. Functional assays showed that the proliferation capacity of SP4 cells correlated with the expression of Qa-2. Raft-disruption on both CD4SP and epithelial cells by cholesterol extraction or cholesterol oxidation in a medullary thymic epithelial cell (mTEC)-supported co-culture system impaired the transition from SP3 to SP4. This result was further confirmed in fetal thymic organ culture system. Collectively, these studies suggest that raft-associated signaling between mTECs and thymocytes drives the differentiation of CD4SP thymocytes and lipid rafts are involved in the final maturation of SP4 thymocytes. (C) 2012 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 30900735 ; 81070271 ; 211012 ; 2011CB946103
项目资助者: National Natural Science Foundation of China ; Key Project of Chinese Ministry of Education ; National Basic Research Program of China
WOS记录号: WOS:000314192500010
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62442
Appears in Collections:基础医学院_免疫学系_期刊论文

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作者单位: 1.Tianjin Med Univ, Dept Immunol, Tianjin Key Lab Cellular & Mol Immunol, Key Lab,Educ Minist, Tianjin 300070, Peoples R China
2.Tianjin Med Univ, Dept Biochem, Tianjin, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Sch Basic Sci, Dept Immunol, Beijing 100871, Peoples R China

Recommended Citation:
Li, Juan,Li, Hai-Dong,Zhang, Yu,et al. Qa-2 associated lipid rafts are indispensable in the final maturation of CD4(+)CD8(-) thymocytes[J]. IMMUNOLOGY LETTERS,2012,148(2):163-171.
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