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学科主题基础医学
Pro-protein convertase-2/carboxypeptidase-E mediated neuropeptide processing of RGC-5 cell after in vitro ischemia
Tang, Song-Shan1; Zhang, Juan-Hui2; Liu, Huan-Xin3; Zhou, Dong4; Qi, Rong5
关键词neuropeptide pro-protein processing system retina ganglion cell-5 in vitro oxygen and glucose deprivation pro-protein convertase-2 carboxypeptidase-E preproneuropeptide-Y
刊名NEUROSCIENCE BULLETIN
2009-02-01
DOI10.1007/s12264-009-1027-8
25期:1页:7-14
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Neurosciences
研究领域[WOS]Neurosciences & Neurology
英文摘要

Objective To observe the change of the neuropeptide pro-protein processing system in the ischemic retina ganglion cell-5 (RGC-5) cells, pro-protein convertase-2 (PC2), carboxypeptidase-E (CPE) and preproneuropeptide Y (preproNPY) protein levels in the ischemic RGC-5 cells and conditioned medium were analyzed. Methods The RGC-5 cell was differentiated in 0.1 mu mol/L staurosporine for 24 h and then stressed by different doses of oxygen and glucose deprivation (OGD). The acute or chronic OGD-induced cell death rates were obtained by using PI or TUNEL staining. The protein expression levels were determined by using the Western blot method and PC2 activity analysis. Results The ischemia caused substantial cell death in an OGD dose-dependent manner. In the cells, proPC2 and preproNPY protein levels gradually increased whereas proCPE gradually decreased. After OGD, PC2 activity was decreased. In the conditioned medium, proPC2 and PC2 proteins gradually decreased whereas proCPE, CPE, and preproNPY proteins gradually increased. Conclusion These results demonstrated that OGD inhibited the neuropeptide pro-protein processing system by reducing PC2 activity and the maturation of proPC2. The aggregation of the pro-proteins and the increase of the active CPE excision adversely exacerbated the cell injury. The pro-protein processing system might play a critical role in the ischemic stress of RGC-5 cells.

语种英语
WOS记录号WOS:000208088900002
项目编号2005SMK22
资助机构Guangdong Pharmaceutical University
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62445
专题北京大学基础医学院_心血管所
北京大学基础医学院
作者单位1.Guangdong Pharmaceut Univ, Dept Biochem, Sch Basic Courses, Guangzhou 510006, Guangdong, Peoples R China
2.Wu Jing Zong Dui Hosp Guangdong Prov, Dept Obstet & Gynaecol, Guangzhou 510507, Guangdong, Peoples R China
3.Wu Jing Zong Dui Hosp Guangdong Prov, Dept Pathol, Guangzhou 510507, Guangdong, Peoples R China
4.Guangdong Gen Hosp, Dept Neurosurg, Guangzhou 510000, Guangdong, Peoples R China
5.Peking Univ, Inst Cardiovasc Sci, Peking Univ Hlth Sci Ctr, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Tang, Song-Shan,Zhang, Juan-Hui,Liu, Huan-Xin,et al. Pro-protein convertase-2/carboxypeptidase-E mediated neuropeptide processing of RGC-5 cell after in vitro ischemia[J]. NEUROSCIENCE BULLETIN,2009,25(1):7-14.
APA Tang, Song-Shan,Zhang, Juan-Hui,Liu, Huan-Xin,Zhou, Dong,&Qi, Rong.(2009).Pro-protein convertase-2/carboxypeptidase-E mediated neuropeptide processing of RGC-5 cell after in vitro ischemia.NEUROSCIENCE BULLETIN,25(1),7-14.
MLA Tang, Song-Shan,et al."Pro-protein convertase-2/carboxypeptidase-E mediated neuropeptide processing of RGC-5 cell after in vitro ischemia".NEUROSCIENCE BULLETIN 25.1(2009):7-14.
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