|Cytokine Expression Profile in Aqueous Humor and Sera of Patients with Acute Anterior Uveitis|
|Chen, W.1; Zhao, B.1; Jiang, R.1; Zhang, R.1; Wang, Y.1; Wu, H.2; Gordon, L.3; Chen, L.1,4|
|关键词||Anterior uveitis aqueous humor cytokines HLA-B27 serum Th1 Th17|
|刊名||CURRENT MOLECULAR MEDICINE|
|WOS标题词||Science & Technology|
|类目[WOS]||Medicine, Research & Experimental|
|研究领域[WOS]||Research & Experimental Medicine|
|关键词[WOS]||GENOME-WIDE ASSOCIATION ; BEHCETS-DISEASE ; TH17 CELLS ; AUTOIMMUNE UVEITIS ; INTERLEUKIN-17 ; INDUCTION ; IL23R-IL12RB2 ; HLA-B27 ; IL-17 ; IL10|
Purpose: To evaluate cytokine expression profile in aqueous humor and sera in patients with HLA-B27 associated acute anterior uveitis (AAU) and idiopathic AAU.
Methods: Twenty patients with AAU and 17 controls were recruited from August 2012 to March 2013. Study subjects with uveitis were divided into two groups: 9 patients with idiopathic AAU and 11 patients with HLA-B27 associated AAU. Complete ophthalmological examinations were performed and clinical features of each group were clearly documented. Aqueous humor and sera were collected and the concentration of 15 immune mediators (IL-1 beta, IL-4, IL-6, IL-10, IL-17A, IL-17F, IL-21, IL-22, IL-23, IL-25, IL-31, IL-33, TNF-alpha, IFN-gamma, sCD40L) were measured in both aqueous humor and sera simultaneously by multiplex immunoassay.
Results: There were significantly higher levels of multiple cytokines in aqueous humor in patients with uveitis compared to controls, including IL-1 beta, IL-6, IL-10, IL-17a, IL-17f, IL-21, IL-25, IL-31, IFN-gamma, TNF-alpha, and sCD40L. The levels of IL-17a in aqueous humor correlated significantly with disease activity in patients with idiopathic AAU, while the level of IFN-gamma in aqueous humor correlated significantly with disease activity in patients with HLA-B27 associated AAU. There was no significant difference in serum cytokine expression between uveitis patients and controls except IL-6, elevated in patients with both idiopathic and HLA-B27 associated AAU.
Conclusion: Cytokine expression pattern in the aqueous humor, in contrast to that in serum, may reflect intraocular immune reactions during active inflammation in patients with AAU. Both Th1 and Th17 are involved in immunopathogenesis of HLA-B27 associated and idiopathic AAU, but a different cytokine pattern was identified in these two clinical entities. A predominant Th17-driven immune response may play an important role in the immunopathogenesis of idiopathic AAU, while Th1 dominant immune response may be responsible for the inflammation in HLA-B27 associated AAU.
|项目编号||81371042 ; 81100636 ; 2013CB967503 ; 81100656/H1203|
|资助机构||National Natural Science Foundation of China ; Natural Science Foundation of China ; Scientific Research Starting Foundation for the Returned Overseas Chinese Scholars, Ministry of Education ; National Natural Science Foundation Project of China|
|作者单位||1.Fudan Univ, Eye & ENT Hosp, Dept Ophthalmol & Vis Sci, Shanghai Med Sch, Shanghai 200433, Peoples R China|
2.Peking Univ, Peoples Hosp, Key Lab Vision Loss & Restorat, Dept Ophthalmol,Minist Educ, Beijing 100871, Peoples R China
3.Univ Calif Los Angeles, Jules Stein Eye Inst, David Geffen Sch Med, Los Angeles, CA 90095 USA
4.Univ Hong Kong, Dept Ophthalmol, Hong Kong, Hong Kong, Peoples R China
|Chen, W.,Zhao, B.,Jiang, R.,et al. Cytokine Expression Profile in Aqueous Humor and Sera of Patients with Acute Anterior Uveitis[J]. CURRENT MOLECULAR MEDICINE,2015,15(6):543-549.|
|APA||Chen, W..,Zhao, B..,Jiang, R..,Zhang, R..,Wang, Y..,...&Chen, L..(2015).Cytokine Expression Profile in Aqueous Humor and Sera of Patients with Acute Anterior Uveitis.CURRENT MOLECULAR MEDICINE,15(6),543-549.|
|MLA||Chen, W.,et al."Cytokine Expression Profile in Aqueous Humor and Sera of Patients with Acute Anterior Uveitis".CURRENT MOLECULAR MEDICINE 15.6(2015):543-549.|