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学科主题临床医学
The effects of endothelial progenitor cells on rat atherosclerosis
Wang, Xianyou1,2; Wang, Feng1,3,4; Li, Nana5; Hu, Min6; Chen, Yun7; Tan, Mengqun1
关键词endothelial progenitor cells atherosclerosis adeno-associated virus-2 gene therapy
刊名BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
2015-03-01
DOI10.1002/bab.1254
62期:2页:186-192
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
研究领域[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
关键词[WOS]APOLIPOPROTEIN-E ; TRANSDUCTION EFFICIENCY ; GENE-THERAPY ; DEFICIENT ; PREVENTS ; DISEASE ; BIOLOGY ; REPAIR ; MICE
英文摘要

Atherosclerosis (AS) is a progressive disease characterized by endothelial injury and lipid aggregation in the arterial walls. Studies have reported that endothelial progenitor cells (EPCs) derived from the bone marrow (BM) might provide an endogenous repair mechanism by differentiating into endothelial cells to replace the dysfunctional endothelium. Our study aims to investigate the effect of EPCs derived from rat BM on AS. EPCs transduced by recombinant adeno-associated virus-green fluorescent protein (GFP) were transplanted into a rat AS model. After 2 months of transplantation, the localization of GFP-labeled cells, morphology, and lipid content in the aorta were examined. GFP-labeled EPCs were found in the endothelial monolayer of the artery vessel in the GFP/EPC group. Hematoxylin and eosin staining suggested that the lipid deposits in the aortic endothelium in the EPC/GFP group were less compared with those in the untreated group. Oil Red O staining of liver slices showed that lipid droplets were obviously decreased in the GFP/EPC group. The endothelial nitric oxide synthase and apolipoprotein E mRNA levels in the GFP/EPC group were significantly higher, but the intercellular cell adhesion molecule-1 mRNA level was significantly lower compared with the control group. The results suggest that EPCs derived from the BM can repair the injured endothelium and promote an atherosclerotic lesion regression. Therefore, EPCs may provide a useful tool for the treatment of AS. (C) 2014 International Union of Biochemistry and Molecular Biology, Inc.

语种英语
WOS记录号WOS:000353164500005
项目编号81341097 ; U1204810 ; 81101048 ; JCYJ20120616144352139
资助机构National Natural Science Foundation ; Shenzhen Basic Research Program
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62466
专题北京大学深圳医院_超声影像科
北京大学临床肿瘤学院_核医学科
作者单位1.Shenzhen Peking Univ, Biomed Res Inst, Shenzhen, Peoples R China
2.Hunan Ctr Dis Control & Prevent, Changsha, Hunan, Peoples R China
3.Hunan Normal Univ, Changsha, Hunan, Peoples R China
4.Xinxiang Med Univ, Dept Physiol & Neurobiol, Xinxiang, Peoples R China
5.Cent S Univ, Xiang Ya Sch Med, Dept Physiol, Expt Hematol Lab, Changsha 410078, Hunan, Peoples R China
6.Xinxiang Med Univ, Key Lab Med Tissue Regenerat Henan Prov, Xinxiang, Peoples R China
7.Peking Univ, Shenzhen Hosp, Dept Ultrasound, Shenzhen, Peoples R China
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GB/T 7714
Wang, Xianyou,Wang, Feng,Li, Nana,et al. The effects of endothelial progenitor cells on rat atherosclerosis[J]. BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY,2015,62(2):186-192.
APA Wang, Xianyou,Wang, Feng,Li, Nana,Hu, Min,Chen, Yun,&Tan, Mengqun.(2015).The effects of endothelial progenitor cells on rat atherosclerosis.BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY,62(2),186-192.
MLA Wang, Xianyou,et al."The effects of endothelial progenitor cells on rat atherosclerosis".BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY 62.2(2015):186-192.
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