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学科主题: 临床医学
题名:
The effects of endothelial progenitor cells on rat atherosclerosis
作者: Wang, Xianyou1,2; Wang, Feng1,3,4; Li, Nana5; Hu, Min6; Chen, Yun7; Tan, Mengqun1
关键词: endothelial progenitor cells ; atherosclerosis ; adeno-associated virus-2 ; gene therapy
刊名: BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
发表日期: 2015-03-01
DOI: 10.1002/bab.1254
卷: 62, 期:2, 页:186-192
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
研究领域[WOS]: Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
关键词[WOS]: APOLIPOPROTEIN-E ; TRANSDUCTION EFFICIENCY ; GENE-THERAPY ; DEFICIENT ; PREVENTS ; DISEASE ; BIOLOGY ; REPAIR ; MICE
英文摘要:

Atherosclerosis (AS) is a progressive disease characterized by endothelial injury and lipid aggregation in the arterial walls. Studies have reported that endothelial progenitor cells (EPCs) derived from the bone marrow (BM) might provide an endogenous repair mechanism by differentiating into endothelial cells to replace the dysfunctional endothelium. Our study aims to investigate the effect of EPCs derived from rat BM on AS. EPCs transduced by recombinant adeno-associated virus-green fluorescent protein (GFP) were transplanted into a rat AS model. After 2 months of transplantation, the localization of GFP-labeled cells, morphology, and lipid content in the aorta were examined. GFP-labeled EPCs were found in the endothelial monolayer of the artery vessel in the GFP/EPC group. Hematoxylin and eosin staining suggested that the lipid deposits in the aortic endothelium in the EPC/GFP group were less compared with those in the untreated group. Oil Red O staining of liver slices showed that lipid droplets were obviously decreased in the GFP/EPC group. The endothelial nitric oxide synthase and apolipoprotein E mRNA levels in the GFP/EPC group were significantly higher, but the intercellular cell adhesion molecule-1 mRNA level was significantly lower compared with the control group. The results suggest that EPCs derived from the BM can repair the injured endothelium and promote an atherosclerotic lesion regression. Therefore, EPCs may provide a useful tool for the treatment of AS. (C) 2014 International Union of Biochemistry and Molecular Biology, Inc.

语种: 英语
所属项目编号: 81341097 ; U1204810 ; 81101048 ; JCYJ20120616144352139
项目资助者: National Natural Science Foundation ; Shenzhen Basic Research Program
WOS记录号: WOS:000353164500005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62466
Appears in Collections:北京大学深圳医院_超声影像科_期刊论文

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作者单位: 1.Shenzhen Peking Univ, Biomed Res Inst, Shenzhen, Peoples R China
2.Hunan Ctr Dis Control & Prevent, Changsha, Hunan, Peoples R China
3.Hunan Normal Univ, Changsha, Hunan, Peoples R China
4.Xinxiang Med Univ, Dept Physiol & Neurobiol, Xinxiang, Peoples R China
5.Cent S Univ, Xiang Ya Sch Med, Dept Physiol, Expt Hematol Lab, Changsha 410078, Hunan, Peoples R China
6.Xinxiang Med Univ, Key Lab Med Tissue Regenerat Henan Prov, Xinxiang, Peoples R China
7.Peking Univ, Shenzhen Hosp, Dept Ultrasound, Shenzhen, Peoples R China

Recommended Citation:
Wang, Xianyou,Wang, Feng,Li, Nana,et al. The effects of endothelial progenitor cells on rat atherosclerosis[J]. BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY,2015,62(2):186-192.
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