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学科主题临床医学
Calcitonin gene-related peptide-enhanced nitric oxide release and inducible NOS activity and mRNA expression in LPS-stimulated mouse peritoneal macrophages
Liu, JG; Chen, MZ; Wang, X
关键词NO CGRP macrophage LPS inducible nitric oxide synthase cGMP immunocytochemistry
刊名SHOCK
2001-07-01
16期:1页:64-69
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Critical Care Medicine ; Hematology ; Surgery ; Peripheral Vascular Disease
研究领域[WOS]General & Internal Medicine ; Hematology ; Surgery ; Cardiovascular System & Cardiology
关键词[WOS]SMOOTH-MUSCLE CELLS ; NF-KAPPA-B ; CYCLIC-AMP ; FUNCTIONAL RECEPTORS ; INTERFERON-GAMMA ; SYNTHASE GENE ; CGRP ; RAT ; INDUCTION ; LIPOPOLYSACCHARIDE
英文摘要

Previously we have shown that calcitonin gene-related peptide (CGRP), a neuropeptide increases lipopolysaccharide-(LPS) induced nitric oxide (NO) production in mouse peritoneal macrophages by using the Griess method. in this study we further examined whether CGRP could modulate inducible NO synthase (iNOS) protein and mRNA expression from mouse peritoneal macrophages. Macrophages were obtained from the peritoneal exude of male Balb/c mouse. The cells were plated on culture dishes at a density of 5 x 10(5) cells per well and were allowed to adhere for P h. After incubation for 24 h, the macrophages were cultured with 0.01 to 1 mug/mL LPS with or without CGRP (1-1,000 nM) for 24 h. The results showed that CGRP markedly enhanced 0.5 mug/mL LPS-induced NO release as compared with that of lower doses of LPS, such as 0.01 and 0.1 mug/mL LPS. NO was increased from 19.8 +/- 2.6 to the highest level of 31.5 +/- 4.2 muM in 5 x 105 cells by 10 nM CGRP in 0.5 mug/mL LPS-stimulated macrophages. The cGMP level in macrophages was augmented when CGRP was added with LPS. However, when using higher dose (1.0 mug/mL) of LPS to stimulate the macrophages, CGRP had no effect at all on NO release. CGRP had no direct effect on NO and cGMP production. CGRP increased the expression of inducible NOS protein in LPS-stimulated macrophages shown by immunocytochemistry method. The activity of iNOS was also enhanced by CGRP as compared with LPS-stimulation alone by detecting the H-3-L-citruline formation from H-3-L-arginine. We found that CGRP also increased the LPS-induced iNOS mRNA levels by using reverse transcriptase-PCR method. These data suggest that CGRP enhances LPS-induced NO release, iNOS activity, and iNOS mRNA in mouse peritoneal macrophages.

语种英语
WOS记录号WOS:000169444200012
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62467
专题北京大学第三临床医学院_心血管内科
作者单位1.Beijing Med Univ, Hosp 3, Inst Vasc Med, Beijing 100083, Peoples R China
2.Cornell Univ, Weill Med Coll, Dept Microbiol & Immunol, New York, NY 10021 USA
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GB/T 7714
Liu, JG,Chen, MZ,Wang, X. Calcitonin gene-related peptide-enhanced nitric oxide release and inducible NOS activity and mRNA expression in LPS-stimulated mouse peritoneal macrophages[J]. SHOCK,2001,16(1):64-69.
APA Liu, JG,Chen, MZ,&Wang, X.(2001).Calcitonin gene-related peptide-enhanced nitric oxide release and inducible NOS activity and mRNA expression in LPS-stimulated mouse peritoneal macrophages.SHOCK,16(1),64-69.
MLA Liu, JG,et al."Calcitonin gene-related peptide-enhanced nitric oxide release and inducible NOS activity and mRNA expression in LPS-stimulated mouse peritoneal macrophages".SHOCK 16.1(2001):64-69.
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