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学科主题: 基础医学
题名:
Taurine antagonized oxidative stress injury induced by homocysteine in rat vascular smooth muscle cells
作者: Chang, L; Xu, JX; Zhao, J; Pang, YZ; Tang, CS; Qi, YF
关键词: homocysteine ; taurine ; vascular smooth muscle ; mitochondria ; reactive oxygen species
刊名: ACTA PHARMACOLOGICA SINICA
发表日期: 2004-03-01
卷: 25, 期:3, 页:341-346
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]: Chemistry ; Pharmacology & Pharmacy
关键词[WOS]: EXTRACELLULAR-SUPEROXIDE DISMUTASE ; MITOCHONDRIAL RESPIRATORY-CHAIN ; HEART ; EXPRESSION ; ISCHEMIA
英文摘要:

AIM: To observe protective effects of taurine on reactive oxygen species generation induced by homocysteine in rat vascular smooth muscle cells (VSMC). METHODS: Rat VSMC was incubated with various concentrations of homocysteine and taurine. The lactate dehydrogenase (LDH) activity which released into culture medium was elevated as an indicator for VSMC injury. The reactive oxygen species (ROS) - hydrogen peroxide (H(2)O(2)) and superoxide anion (W.)were measured with luminol or lucigenin chemiluminescences method, and the mitochondria Mn-superoxide dismutase (Mn-SOD) and catalase (CAT) were also measured in treated VSMC. RESULTS: LDH leakage from cultured VSMC treated with homocystenie, was increased (P<0.01 vs control), and it was markedly inhibited when co-incubated with taurine (P<0.01). Homocysteine induced H(2)O(2), generation from VSMC in a concentration dependent manner (P<0.01 vs control). However, taurine (5, 10, and 20 mmol/L) significantly antagonized 0.5 mmol/L homocysteine-induced H(2)O(2) generation in VSMC in a concentration dependent manner (P < 0.01 vs C 202 generation in VSMC (P > 0.05 vs control). homocysteine alone group), although taurine itself did not alter the H In this study, the superoxide anion in VSMC was not detectable by chemiluminent method. In addition, treatment of VSMC with taurine increased mitochondria Mn-SOD and CAT activity in a concentration dependent manner (P < 0.05). but homocysteine decreased mitochondria Mn-SOD and CAT activity (P<0.01 vs control). In addition, co-administration of taurine markedly ameliorated homocysteine-induced inhibition of Mn-SOD and CAT activity in VSMC (P<0.01 vs homocysteine alone group). CONCLUSION: Taurine antagonized the effects of homocysteine on ROS generation and anti-oxidant enzyme activities in rat VSMC in vitro.

语种: 英语
WOS记录号: WOS:000220117800015
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62482
Appears in Collections:基础医学院_生理学与病理生理学系_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Dept Physiol, Beijing 100083, Peoples R China
2.Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China
3.Peking Univ, Inst Cardiovasc Dis, Beijing 100083, Peoples R China
4.Peking Univ, Hosp 1, Inst Cardiovasc Dis, Beijing 100034, Peoples R China

Recommended Citation:
Chang, L,Xu, JX,Zhao, J,et al. Taurine antagonized oxidative stress injury induced by homocysteine in rat vascular smooth muscle cells[J]. ACTA PHARMACOLOGICA SINICA,2004,25(3):341-346.
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