学科主题基础医学
Taurine antagonized oxidative stress injury induced by homocysteine in rat vascular smooth muscle cells
Chang, L; Xu, JX; Zhao, J; Pang, YZ; Tang, CS; Qi, YF
关键词homocysteine taurine vascular smooth muscle mitochondria reactive oxygen species
刊名ACTA PHARMACOLOGICA SINICA
2004-03-01
25期:3页:341-346
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]Chemistry ; Pharmacology & Pharmacy
关键词[WOS]EXTRACELLULAR-SUPEROXIDE DISMUTASE ; MITOCHONDRIAL RESPIRATORY-CHAIN ; HEART ; EXPRESSION ; ISCHEMIA
英文摘要

AIM: To observe protective effects of taurine on reactive oxygen species generation induced by homocysteine in rat vascular smooth muscle cells (VSMC). METHODS: Rat VSMC was incubated with various concentrations of homocysteine and taurine. The lactate dehydrogenase (LDH) activity which released into culture medium was elevated as an indicator for VSMC injury. The reactive oxygen species (ROS) - hydrogen peroxide (H(2)O(2)) and superoxide anion (W.)were measured with luminol or lucigenin chemiluminescences method, and the mitochondria Mn-superoxide dismutase (Mn-SOD) and catalase (CAT) were also measured in treated VSMC. RESULTS: LDH leakage from cultured VSMC treated with homocystenie, was increased (P<0.01 vs control), and it was markedly inhibited when co-incubated with taurine (P<0.01). Homocysteine induced H(2)O(2), generation from VSMC in a concentration dependent manner (P<0.01 vs control). However, taurine (5, 10, and 20 mmol/L) significantly antagonized 0.5 mmol/L homocysteine-induced H(2)O(2) generation in VSMC in a concentration dependent manner (P < 0.01 vs C 202 generation in VSMC (P > 0.05 vs control). homocysteine alone group), although taurine itself did not alter the H In this study, the superoxide anion in VSMC was not detectable by chemiluminent method. In addition, treatment of VSMC with taurine increased mitochondria Mn-SOD and CAT activity in a concentration dependent manner (P < 0.05). but homocysteine decreased mitochondria Mn-SOD and CAT activity (P<0.01 vs control). In addition, co-administration of taurine markedly ameliorated homocysteine-induced inhibition of Mn-SOD and CAT activity in VSMC (P<0.01 vs homocysteine alone group). CONCLUSION: Taurine antagonized the effects of homocysteine on ROS generation and anti-oxidant enzyme activities in rat VSMC in vitro.

语种英语
WOS记录号WOS:000220117800015
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62482
专题北京大学基础医学院_生理学与病理生理学系
作者单位1.Peking Univ, Hlth Sci Ctr, Dept Physiol, Beijing 100083, Peoples R China
2.Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China
3.Peking Univ, Inst Cardiovasc Dis, Beijing 100083, Peoples R China
4.Peking Univ, Hosp 1, Inst Cardiovasc Dis, Beijing 100034, Peoples R China
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Chang, L,Xu, JX,Zhao, J,et al. Taurine antagonized oxidative stress injury induced by homocysteine in rat vascular smooth muscle cells[J]. ACTA PHARMACOLOGICA SINICA,2004,25(3):341-346.
APA Chang, L,Xu, JX,Zhao, J,Pang, YZ,Tang, CS,&Qi, YF.(2004).Taurine antagonized oxidative stress injury induced by homocysteine in rat vascular smooth muscle cells.ACTA PHARMACOLOGICA SINICA,25(3),341-346.
MLA Chang, L,et al."Taurine antagonized oxidative stress injury induced by homocysteine in rat vascular smooth muscle cells".ACTA PHARMACOLOGICA SINICA 25.3(2004):341-346.
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