Molecular detection of EWS-Ets fusion transcripts and their clinicopathologic significance in Ewing&prime;s sarcoma/peripheral primitive neuroectodermal tumor

IR@PKUHSC > 北京大学基础医学院 > 病理学系

学科主题	基础医学
	Molecular detection of EWS-Ets fusion transcripts and their clinicopathologic significance in Ewing′s sarcoma/peripheral primitive neuroectodermal tumor
	Wang, H; Zheng, J; Wang, YP; Yang, Y; You, JF
关键词	Ewing&prime s sarcoma/peripheral primitive neuroectodermal tumor gene fusion reverse transcriptase-polymerase chain reaction
刊名	CHINESE MEDICAL JOURNAL
	2005-08-20
卷	118 期:16 页:1323-1329
收录类别	SCI
文章类型	Article
WOS标题词	Science & Technology
类目[WOS]	Medicine, General & Internal
研究领域[WOS]	General & Internal Medicine
关键词[WOS]	PARAFFIN-EMBEDDED TISSUE ; ROUND-CELL TUMORS ; SARCOMA TRANSLOCATION ; CHIMERIC TRANSCRIPTS ; MIC2 EXPRESSION ; DNA-BINDING ; FAMILY ; GENE ; PROTEINS ; FUSES
英文摘要	Background Ewing′s sarcoma/peripheral primitive neuroectodermal tumor (ES/pPNET) is often difficult to distinguish from other small round cell tumors. The EWS-Ets gene fusions that result from chromosomal translocations in this tumor provide potential molecular diagnostic markers. To apply these molecular markers to commonly available archival materials, we evaluated the feasibility of detecting EWS-Ets including EWS-Flil and EWS-ERG fusion transcripts in paraffin-embedded tissues and its diagnostic value for detecting ES/pPNET. Methods Thirteen paraffin-embedded samples of ES/pPNETs were retrieved from archives. Thirteen cases of other tumors with small round cell features (including rhabdomyosarcoma, neuroblastoma, lymphoma, small cell carcinoma, and desmoplastic small round cell tumor) were used as negative controls. beta-actin and beta(2)-microglobulin were used as internal controls. A nested reverse transcriptase-polymerase chain reaction ( RTPCR)-based assay was performed to detect the EWS-Flil and EWS-ERG fusion transcripts. Results beta-actin and beta(2)-microglobulin were detected in 10/13 and 13/13 ES/pPNETs, respectively. EWS-Fli1 fusion transcripts were detected in 11 of 13 (85%) ES/pPNETs. Three chimeric transcripts, all EWS-Flil, were detected in ES/pPNET samples. Among 11 EWS-Fli1-positive cases, 7 cases had a type I fusion transcript involving fusion of EWS exon 7 with Flit exon 6, 2 cases had a type H fusion transcript involving EWS exon 7 with Hit exon 5, and 2 cases expressed fusion transcripts involving EWS exon 7 and Flit exon 8. Type I EWS-Fli1 fusion predominated over other types. Fusion types could not be distinguished in the remaining 2 cases. Thirteen negative controls did not show detectable chimeric messages. There was a significant relationship between EWS-Flil fusion transcripts and CD99 expression. Conclusions Molecular detection of EWS-Flil fusion transcripts in formalin-fixed paraffin-embedded material by nested RT-PCR is feasible and is useful for the diagnosis and differential diagnosis of ES/pPNETs.
语种	英语
WOS记录号	WOS:000231555000001
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.bjmu.edu.cn/handle/400002259/62497
专题	北京大学基础医学院_病理学系
作者单位	Peking Univ, Hlth Sci Ctr, Dept Pathol, Beijing 100083, Peoples R China
推荐引用方式 GB/T 7714	Wang, H,Zheng, J,Wang, YP,et al. Molecular detection of EWS-Ets fusion transcripts and their clinicopathologic significance in Ewing′s sarcoma/peripheral primitive neuroectodermal tumor[J]. CHINESE MEDICAL JOURNAL,2005,118(16):1323-1329.
APA	Wang, H,Zheng, J,Wang, YP,Yang, Y,&You, JF.(2005).Molecular detection of EWS-Ets fusion transcripts and their clinicopathologic significance in Ewing′s sarcoma/peripheral primitive neuroectodermal tumor.CHINESE MEDICAL JOURNAL,118(16),1323-1329.
MLA	Wang, H,et al."Molecular detection of EWS-Ets fusion transcripts and their clinicopathologic significance in Ewing′s sarcoma/peripheral primitive neuroectodermal tumor".CHINESE MEDICAL JOURNAL 118.16(2005):1323-1329.