IR@PKUHSC  > 北京大学基础医学院  > 病理学系
学科主题基础医学
Molecular detection of EWS-Ets fusion transcripts and their clinicopathologic significance in Ewing′s sarcoma/peripheral primitive neuroectodermal tumor
Wang, H; Zheng, J; Wang, YP; Yang, Y; You, JF
关键词Ewing&prime s sarcoma/peripheral primitive neuroectodermal tumor gene fusion reverse transcriptase-polymerase chain reaction
刊名CHINESE MEDICAL JOURNAL
2005-08-20
118期:16页:1323-1329
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
研究领域[WOS]General & Internal Medicine
关键词[WOS]PARAFFIN-EMBEDDED TISSUE ; ROUND-CELL TUMORS ; SARCOMA TRANSLOCATION ; CHIMERIC TRANSCRIPTS ; MIC2 EXPRESSION ; DNA-BINDING ; FAMILY ; GENE ; PROTEINS ; FUSES
英文摘要

Background Ewing′s sarcoma/peripheral primitive neuroectodermal tumor (ES/pPNET) is often difficult to distinguish from other small round cell tumors. The EWS-Ets gene fusions that result from chromosomal translocations in this tumor provide potential molecular diagnostic markers. To apply these molecular markers to commonly available archival materials, we evaluated the feasibility of detecting EWS-Ets including EWS-Flil and EWS-ERG fusion transcripts in paraffin-embedded tissues and its diagnostic value for detecting ES/pPNET.

Methods Thirteen paraffin-embedded samples of ES/pPNETs were retrieved from archives. Thirteen cases of other tumors with small round cell features (including rhabdomyosarcoma, neuroblastoma, lymphoma, small cell carcinoma, and desmoplastic small round cell tumor) were used as negative controls. beta-actin and beta(2)-microglobulin were used as internal controls. A nested reverse transcriptase-polymerase chain reaction ( RTPCR)-based assay was performed to detect the EWS-Flil and EWS-ERG fusion transcripts.

Results beta-actin and beta(2)-microglobulin were detected in 10/13 and 13/13 ES/pPNETs, respectively. EWS-Fli1 fusion transcripts were detected in 11 of 13 (85%) ES/pPNETs. Three chimeric transcripts, all EWS-Flil, were detected in ES/pPNET samples. Among 11 EWS-Fli1-positive cases, 7 cases had a type I fusion transcript involving fusion of EWS exon 7 with Flit exon 6, 2 cases had a type H fusion transcript involving EWS exon 7 with Hit exon 5, and 2 cases expressed fusion transcripts involving EWS exon 7 and Flit exon 8. Type I EWS-Fli1 fusion predominated over other types. Fusion types could not be distinguished in the remaining 2 cases. Thirteen negative controls did not show detectable chimeric messages. There was a significant relationship between EWS-Flil fusion transcripts and CD99 expression.

Conclusions Molecular detection of EWS-Flil fusion transcripts in formalin-fixed paraffin-embedded material by nested RT-PCR is feasible and is useful for the diagnosis and differential diagnosis of ES/pPNETs.

语种英语
WOS记录号WOS:000231555000001
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62497
专题北京大学基础医学院_病理学系
作者单位Peking Univ, Hlth Sci Ctr, Dept Pathol, Beijing 100083, Peoples R China
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GB/T 7714
Wang, H,Zheng, J,Wang, YP,et al. Molecular detection of EWS-Ets fusion transcripts and their clinicopathologic significance in Ewing′s sarcoma/peripheral primitive neuroectodermal tumor[J]. CHINESE MEDICAL JOURNAL,2005,118(16):1323-1329.
APA Wang, H,Zheng, J,Wang, YP,Yang, Y,&You, JF.(2005).Molecular detection of EWS-Ets fusion transcripts and their clinicopathologic significance in Ewing′s sarcoma/peripheral primitive neuroectodermal tumor.CHINESE MEDICAL JOURNAL,118(16),1323-1329.
MLA Wang, H,et al."Molecular detection of EWS-Ets fusion transcripts and their clinicopathologic significance in Ewing′s sarcoma/peripheral primitive neuroectodermal tumor".CHINESE MEDICAL JOURNAL 118.16(2005):1323-1329.
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