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学科主题: 临床医学
题名:
Upregulated periostin promotes angiogenesis in keloids through activation of the ERK 1/2 and focal adhesion kinase pathways, as well as the upregulated expression of VEGF and angiopoietin-1
作者: Zhang, Zhe; Nie, Fangfei; Chen, Xinlei; Qin, Zelian; Kang, Chunfu; Chen, Bin; Ma, Jianxun; Pan, Bolin; Ma, Yongguang
关键词: periostin ; fibroblast ; keloid ; endothelial cells ; angiogenesis
刊名: MOLECULAR MEDICINE REPORTS
发表日期: 2015-02-01
DOI: 10.3892/mmr.2014.2827
卷: 11, 期:2, 页:857-864
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology ; Medicine, Research & Experimental
研究领域[WOS]: Oncology ; Research & Experimental Medicine
关键词[WOS]: ENDOTHELIAL GROWTH-FACTOR ; HYPERTROPHIC SCARS ; CANCER-CELLS ; FIBROBLASTS ; PROLIFERATION ; APOPTOSIS ; ETIOLOGY
英文摘要:

Periostin, a secreted extracellular matrix protein, is highly expressed in wound healing and in various types of human cancer and is involved in angiogenesis. Keloids, considered dermal benign tumors, are granulomatous lesions characterized by capillary proliferation. However, the underlying regulatory mechanism of angiogenesis in keloids remains to be elucidated. The present study aimed to examine the effect of periostin on angiogenesis in keloids. The expression of periostin was upregulated and the vessel density was higher in human keloids compared with normal tissue, observed following staining with CD31 and CD105. Periostin demonstrated a markedly positive correlation with blood vessel density, which was assessed using CD31 staining (r=0.711; P<0.01) and a weak correlation was observed using CD105 staining (r=0.251; P<0.01). Conditioned medium from keloid fibroblasts (KFs) promoted the migration and tube formation of human umbilical vein endothelial cells (HUVECs) compared with normal fibroblasts and this effect may have been abrogated by the short hairpin RNA knockdown of periostin. Treatment with recombinant human periostin promoted the migration and tube formation of HUVECs by activating the extracellular signal-regulated kinase 1/2 and focal adhesion kinase signaling pathway. In addition, periostin increased the secretion of vascular endothelial growth factor and angiopoietin-1 in the KFs. In conclusion, these data suggested that upregulation in the level of periostin may promote angiogenesis directly and indirectly in keloids and may be a key factor in keloid development. Periostin may, therefore, be a promising therapeutic target in the treatment of keloids and other angioproliferative diseases.

语种: 英语
所属项目编号: 30973126 ; 20130001110095
项目资助者: National Natural Science Foundation of China ; Ministry of Education Doctoral Foundation of the People&prime ; s Republic of China
WOS记录号: WOS:000349506400014
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62517
Appears in Collections:北京大学第三临床医学院_成形外科_期刊论文

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作者单位: Peking Univ Third Hosp, Dept Plast Surg, Beijing 100191, Peoples R China

Recommended Citation:
Zhang, Zhe,Nie, Fangfei,Chen, Xinlei,et al. Upregulated periostin promotes angiogenesis in keloids through activation of the ERK 1/2 and focal adhesion kinase pathways, as well as the upregulated expression of VEGF and angiopoietin-1[J]. MOLECULAR MEDICINE REPORTS,2015,11(2):857-864.
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