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Evaluation of MAGE-1 and MAGE-3 as tumour-specific markers to detect blood dissemination of hepatocellular carcinoma cells
Mou, DC; Cai, SL; Peng, JR; Wang, Y; Chen, HS; Pang, XW; Leng, XS; Chen, WF
关键词circulating tumour cells hepatocellular carcinoma MAGE transcripts nested polymerase chain reaction tumour-specific marker
刊名BRITISH JOURNAL OF CANCER
2002-01-07
86期:1页:110-116
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]POLYMERASE-CHAIN-REACTION ; FETOPROTEIN MESSENGER-RNA ; PERIPHERAL-BLOOD ; MELANOMA-CELLS ; GENE FAMILY ; CIRCULATING HEPATOCYTES ; HIGH-FREQUENCY ; EXPRESSION ; DISEASE ; HEPATOMA
英文摘要

The members of MAGE gene family are highly expressed in human hepatocellular carcinoma (HCC). In the present study, we tested the tumour-specific MAGE-1 and MAGE-3 transcripts in the peripheral blood of HCC patients by nested RT-PCR to detect the circulating tumour cells and evaluate their potential clinical implication. Of 30 HCC patients, the positive rate of MAGE-1 and MAGE-3 transcripts was 43.3% (13 out of 30) and 33.3% (10 cut of 30) in PBMC samples, whilst the positive rate was 70% (21 out of 30) and 53.3% (16 out of 30) in the resected HCC tissue samples, respectively. The positivity for at least one MAGE gene transcript was 63.3% (19 out of 30) in PBMC samples of HCC patients and 83,396 (25 out of 30) in the resected HCC tissue samples. MAGE-1 and/or MAGE-3 mRNA were not detected in the PBMC of those patients from whom the resected HCC tissues were MAGE-1 or MAGE-3 mRNA negative, nor in the 25 PBMC samples from healthy donors. The detection of MAGE transcripts in PBMC was correlated with the advanced stages and tumour size of the HCC, being 82.4% (14 out of 17) in tumour stages III and IVa, 56.6% (five out of nine) in stage II, and null (nought out of four) in stage I. The serum alpha-FP in 33.3% (10 out of 30) of HCC patients was normal or slightly elevated (<40 ng ml(-1)). However, six of these 10 patients (alpha-FP < 40 ng ml(-1)) were MAGE-1 and /or MAGE-3 mRNA positive in their PBMC, The follow-up survey of MAGE mRNA in PBMC was performed in 12 patients. Seven patients with persistent MAGE-I and/or MAGE-3 mRNA positive or from negative turned to positive died because of metastasis and/or recurrence, in striking contrast, all four patients with MAGE-1 and/or MAGE-3 mRNA from positive turned to negative and one patient with persistent MAGE-3 transcript negative are alive after last test Collectively, detection of MAGE transcripts with follow-up survey in PBMC is a feasible and reliable assay for the early prediction of the relapse and prognosis of the HCC patients.

语种英语
WOS记录号WOS:000174150200021
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被引频次:46[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
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条目标识符http://ir.bjmu.edu.cn/handle/400002259/62519
专题基础医学院_免疫学系
作者单位1.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Immunol, Beijing 100083, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Peoples Hosp, Inst Hepatol, Beijing 100044, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Peoples Hosp, Ctr Hepatobiliary Surg, Beijing 100044, Peoples R China
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Mou, DC,Cai, SL,Peng, JR,et al. Evaluation of MAGE-1 and MAGE-3 as tumour-specific markers to detect blood dissemination of hepatocellular carcinoma cells[J]. BRITISH JOURNAL OF CANCER,2002,86(1):110-116.
APA Mou, DC.,Cai, SL.,Peng, JR.,Wang, Y.,Chen, HS.,...&Chen, WF.(2002).Evaluation of MAGE-1 and MAGE-3 as tumour-specific markers to detect blood dissemination of hepatocellular carcinoma cells.BRITISH JOURNAL OF CANCER,86(1),110-116.
MLA Mou, DC,et al."Evaluation of MAGE-1 and MAGE-3 as tumour-specific markers to detect blood dissemination of hepatocellular carcinoma cells".BRITISH JOURNAL OF CANCER 86.1(2002):110-116.
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