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学科主题: 基础医学
题名:
Forskolin cooperating with growth factor on generation of dopaminergic neurons from human fetal mesencephalic neural progenitor cells
作者: Wang, X; Li, XX; Wang, K; Zhou, HF; Xue, B; Li, LS; Wang, XM
关键词: neural progenitor cells ; dopamine ; forskolin ; fibroblast growth factor 8 ; differentiation ; Parkinson&prime ; s disease
刊名: NEUROSCIENCE LETTERS
发表日期: 2004-05-20
DOI: 10.1016/j.neulet.2004.03.007
卷: 362, 期:2, 页:117-121
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Neurosciences
研究领域[WOS]: Neurosciences & Neurology
关键词[WOS]: HYDROXYLASE GENE-EXPRESSION ; CENTRAL-NERVOUS-SYSTEM ; TYROSINE-HYDROXYLASE ; STEM-CELLS ; PROTEIN-KINASE ; BRAIN NEURONS ; DIFFERENTIATION ; PRECURSORS ; RAT ; TRANSPLANTATION
英文摘要:

Forskolin was tested for its co-activating ability to enhance the function of fibroblast growth factor (FGF) 8 on dopaminergic (DAergic) differentiation from human fetal mesencephalic neural progenitor cells (NPCs). When NPCs were treated with FGF8 alone, the DAergic phenotype was expressed lightly. The addition of 10 muM forskolin increased the number of DAergic neurons, cooperating with 50 ng/ml FGF8. These cells produced neurotransinitter DA, which was measured by high-performance liquid chromatography. Reverse transcriptase-polymerase chain reaction analysis demonstrated that differentiated cells expressed DAergic development-relative genes tyrosine hydroxylase (TH), nuclear receptor-related factor 1 (Nurr1) and D2 receptor (D2R), indicating that matured DAergic neurons could be obtained under these present conditions. The results suggest that forskolin plus FGF8 may contribute to more efficient production of DAergic neurons from human-derived NPCs for therapy of neurodegenerative, diseases. (C) 2004 Elsevier Ireland Ltd. All rights reserved.

语种: 英语
WOS记录号: WOS:000221566400012
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62520
Appears in Collections:基础医学院_神经生物学系_期刊论文

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作者单位: 1.Peking Univ, Neurosci Res Inst, Beijing 100083, Peoples R China
2.Peking Univ, Stem Cell Res Ctr, Beijing 100083, Peoples R China
3.Capital Univ Med Sci, Dept Physiol, Beijing 100054, Peoples R China

Recommended Citation:
Wang, X,Li, XX,Wang, K,et al. Forskolin cooperating with growth factor on generation of dopaminergic neurons from human fetal mesencephalic neural progenitor cells[J]. NEUROSCIENCE LETTERS,2004,362(2):117-121.
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