IR@PKUHSC  > 北京大学基础医学院  > 北京大学衰老研究中心
学科主题基础医学
The tRNA methyltransferase NSun2 stabilizes p16(INK4) mRNA by methylating the 3 ′-untranslated region of p16
Zhang, Xiaotian1; Liu, Zhenyun1; Yi, Jie1; Tang, Hao1; Xing, Junyue1; Yu, Minqwei2; Tong, Tanjun1; Shang, Yongfeng1; Gorospe, Myriam3; Wang, Wengong1
刊名NATURE COMMUNICATIONS
2012-03-01
DOI10.1038/ncomms1692
3
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
资助者Major State Basic Research Development Program of China ; National Science Foundation of China ; Ministry of Education of People&prime ; s Republic of China ; National Institute on Aging-IRP, National Institutes of Health ; Major State Basic Research Development Program of China ; National Science Foundation of China ; Ministry of Education of People&prime ; s Republic of China ; National Institute on Aging-IRP, National Institutes of Health
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]BINDING PROTEIN HUR ; 16S RIBOSOMAL-RNA ; ESCHERICHIA-COLI ; REPLICATIVE SENESCENCE ; MISU NSUN2 ; TRANSLATION ; EXPRESSION ; INITIATION ; RESIDUES ; TARGET
英文摘要

The impact of methylation of the 3′-untranslated region (UTR) of a messenger RNA (mRNA) remains largely unknown. Here we show that NSun2, a transfer RNA methyltransferase, inhibits the turnover of p16(INK4) mRNA. Knockdown of NSun2 reduces p16 expression by shortening the half-life of the p16 mRNA, while overexpression of NSun2 stabilizes the p16 mRNA. In vitro methylation assays show that NSun2 methylates the p16 3′UTR at A988. Knockdown of NSun2 reduces the stability of the EGFP-p16 chimeric reporter transcripts bearing wild-type p16 3′UTR, but not p16 3′UTR with a mutant methylation site. Methylation by NSun2 prevents the association of p16 3′UTR with HuR, AUF1 and Ago2/RISC, and prevents the recruitment of EGFP-p16 3′UTR chimeric transcripts to processing bodies. In response to oxidative stress, NSun2 is essential for elevating p16 expression levels. We conclude that NSun2-mediated methylation of the p16 3′UTR is a novel mechanism to stabilize p16 mRNA.

语种英语
所属项目编号2007CB507400 ; 81070247 ; 30921062 ; 30973147 ; B07001
资助者Major State Basic Research Development Program of China ; National Science Foundation of China ; Ministry of Education of People&prime ; s Republic of China ; National Institute on Aging-IRP, National Institutes of Health ; Major State Basic Research Development Program of China ; National Science Foundation of China ; Ministry of Education of People&prime ; s Republic of China ; National Institute on Aging-IRP, National Institutes of Health
WOS记录号WOS:000302630100001
Citation statistics
Cited Times:45[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62524
Collection北京大学基础医学院_北京大学衰老研究中心
作者单位1.Wuhan Univ, Clin Coll 1, Wuhan 430060, Peoples R China
2.Peking Univ, Dept Biochem & Mol Biol, Res Ctr Aging, Hlth Sci Ctr, Beijing 100191, Peoples R China
3.NIA, Lab Mol Biol & Immunol, NIH, Baltimore, MD 21224 USA
Recommended Citation
GB/T 7714
Zhang, Xiaotian,Liu, Zhenyun,Yi, Jie,et al. The tRNA methyltransferase NSun2 stabilizes p16(INK4) mRNA by methylating the 3 ′-untranslated region of p16[J]. NATURE COMMUNICATIONS,2012,3.
APA Zhang, Xiaotian.,Liu, Zhenyun.,Yi, Jie.,Tang, Hao.,Xing, Junyue.,...&Wang, Wengong.(2012).The tRNA methyltransferase NSun2 stabilizes p16(INK4) mRNA by methylating the 3 ′-untranslated region of p16.NATURE COMMUNICATIONS,3.
MLA Zhang, Xiaotian,et al."The tRNA methyltransferase NSun2 stabilizes p16(INK4) mRNA by methylating the 3 ′-untranslated region of p16".NATURE COMMUNICATIONS 3(2012).
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