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Decreased expression of cystic fibrosis transmembrane conductance regulator impairs sperm quality in aged men
Diao, Ruiying1,2; Fok, Kin Lam3; Zhao, Li4; Chen, Hao1,2; Tang, Hui5; Chen, Jing1; Zheng, Aiping6; Zhang, Xiaohu3; Gui, Yaoting1; Chan, Hsiao Chang3; Cai, Zhiming2
刊名REPRODUCTION
2013-12-01
DOI10.1530/REP-13-0146
146期:6页:637-645
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Developmental Biology ; Reproductive Biology
研究领域[WOS]Developmental Biology ; Reproductive Biology
关键词[WOS]SOLUBLE ADENYLYL-CYCLASE ; SEMEN QUALITY ; FERTILIZING-CAPACITY ; VAS-DEFERENS ; MOUSE SPERM ; CFTR ; SPERMATOZOA ; INVOLVEMENT ; ACTIVATION ; GENE
英文摘要

Sperm quality declines with aging; however, the underlying molecular mechanism remains elusive. The cystic fibrosis transmembrane conductance regulator (CFTR) has been shown to play an essential role in fertilizing capacity of sperm and male fertility. This study aimed to investigate the involvement of age-dependent CFTR downregulation in lowering sperm quality in old age. Two hundred and one healthy fertile men of three age groups (20-40 years, n=64; 40-60 years, n=61; and >60 years, n=76) were recruited. Expression of CFTR was determined by RT-PCR, western blot, and immunofluorescence staining. Collected sperm were treated with CFTR inhibitor or potentiator. Sperm quality was assessed by motility and bicarbonate-induced capacitation. The results showed that the expression of CFTR on the equatorial segment and neck region of sperm was significantly decreased in an age-dependent manner. Reduction of CFTR expression in sperm from old men was correlated with lowered forward motility and decreased HCO3- sensitivity required for sperm capacitation. Activation of CFTR by genistein partially rescued the decreased forward motility in sperm from old men. Decreased CFTR expression in sperm was also found to be associated with lowered sperm quality in aging mice. These results suggest that age-dependent downregulation of CFTR in sperm leads to lowered sperm quality in old age sperm. CFTR may be a pontential target for rescuing sperm motility as well as a fertility indicator in old age men.

语种英语
WOS记录号WOS:000327320500018
项目编号2011CB944303 ; 81070529 ; 31271244 ; 31100841 ; 81270740 ; CXB201104220045A ; 200901015 ; 201202035 ; CXC201005260001A
资助机构National Key Scientific Program of China ; National Natural Science Foundation of China ; Promotion Program for Shenzhen Key Laboratory ; Shenzhen Projects of Science and Technology ; Biobank of Complex Diseases in Shenzhen
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62549
专题北京大学深圳医院
北京大学基础医学院
作者单位1.Peking Univ, Shenzhen Hosp, Shenzhen PKU HKUST Med Ctr, Guangdong & Shenzhen Key Lab Male Reprod Med & Ge, Shenzhen 518036, Peoples R China
2.Shenzhen Univ, Shenzhen Peoples Hosp 2, Affiliated Hosp 1, Shenzhen Key Lab Genitourinary Tumor, Shenzhen 518035, Peoples R China
3.Chinese Univ Hong Kong, Sch Biomed Sci, Epithelial Cell Biol Res Ctr, Fac Med, Hong Kong, Hong Kong, Peoples R China
4.Southern Med Univ, Peoples Hosp Shilong, Dept Lab Med, Dongguan 523321, Peoples R China
5.Peking Univ, Shenzhen Hosp, Dept Lab Med, Shenzhen 518036, Peoples R China
6.Peking Univ, Shenzhen Hosp, Dept Pathol, Shenzhen 518036, Peoples R China
推荐引用方式
GB/T 7714
Diao, Ruiying,Fok, Kin Lam,Zhao, Li,et al. Decreased expression of cystic fibrosis transmembrane conductance regulator impairs sperm quality in aged men[J]. REPRODUCTION,2013,146(6):637-645.
APA Diao, Ruiying.,Fok, Kin Lam.,Zhao, Li.,Chen, Hao.,Tang, Hui.,...&Cai, Zhiming.(2013).Decreased expression of cystic fibrosis transmembrane conductance regulator impairs sperm quality in aged men.REPRODUCTION,146(6),637-645.
MLA Diao, Ruiying,et al."Decreased expression of cystic fibrosis transmembrane conductance regulator impairs sperm quality in aged men".REPRODUCTION 146.6(2013):637-645.
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