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学科主题: 临床医学
题名:
Decreased expression of cystic fibrosis transmembrane conductance regulator impairs sperm quality in aged men
作者: Diao, Ruiying1,2; Fok, Kin Lam3; Zhao, Li4; Chen, Hao1,2; Tang, Hui5; Chen, Jing1; Zheng, Aiping6; Zhang, Xiaohu3; Gui, Yaoting1; Chan, Hsiao Chang3; Cai, Zhiming2
刊名: REPRODUCTION
发表日期: 2013-12-01
DOI: 10.1530/REP-13-0146
卷: 146, 期:6, 页:637-645
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Developmental Biology ; Reproductive Biology
研究领域[WOS]: Developmental Biology ; Reproductive Biology
关键词[WOS]: SOLUBLE ADENYLYL-CYCLASE ; SEMEN QUALITY ; FERTILIZING-CAPACITY ; VAS-DEFERENS ; MOUSE SPERM ; CFTR ; SPERMATOZOA ; INVOLVEMENT ; ACTIVATION ; GENE
英文摘要:

Sperm quality declines with aging; however, the underlying molecular mechanism remains elusive. The cystic fibrosis transmembrane conductance regulator (CFTR) has been shown to play an essential role in fertilizing capacity of sperm and male fertility. This study aimed to investigate the involvement of age-dependent CFTR downregulation in lowering sperm quality in old age. Two hundred and one healthy fertile men of three age groups (20-40 years, n=64; 40-60 years, n=61; and >60 years, n=76) were recruited. Expression of CFTR was determined by RT-PCR, western blot, and immunofluorescence staining. Collected sperm were treated with CFTR inhibitor or potentiator. Sperm quality was assessed by motility and bicarbonate-induced capacitation. The results showed that the expression of CFTR on the equatorial segment and neck region of sperm was significantly decreased in an age-dependent manner. Reduction of CFTR expression in sperm from old men was correlated with lowered forward motility and decreased HCO3- sensitivity required for sperm capacitation. Activation of CFTR by genistein partially rescued the decreased forward motility in sperm from old men. Decreased CFTR expression in sperm was also found to be associated with lowered sperm quality in aging mice. These results suggest that age-dependent downregulation of CFTR in sperm leads to lowered sperm quality in old age sperm. CFTR may be a pontential target for rescuing sperm motility as well as a fertility indicator in old age men.

语种: 英语
所属项目编号: 2011CB944303 ; 81070529 ; 31271244 ; 31100841 ; 81270740 ; CXB201104220045A ; 200901015 ; 201202035 ; CXC201005260001A
项目资助者: National Key Scientific Program of China ; National Natural Science Foundation of China ; Promotion Program for Shenzhen Key Laboratory ; Shenzhen Projects of Science and Technology ; Biobank of Complex Diseases in Shenzhen
WOS记录号: WOS:000327320500018
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62549
Appears in Collections:北京大学深圳医院_期刊论文

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作者单位: 1.Peking Univ, Shenzhen Hosp, Shenzhen PKU HKUST Med Ctr, Guangdong & Shenzhen Key Lab Male Reprod Med & Ge, Shenzhen 518036, Peoples R China
2.Shenzhen Univ, Shenzhen Peoples Hosp 2, Affiliated Hosp 1, Shenzhen Key Lab Genitourinary Tumor, Shenzhen 518035, Peoples R China
3.Chinese Univ Hong Kong, Sch Biomed Sci, Epithelial Cell Biol Res Ctr, Fac Med, Hong Kong, Hong Kong, Peoples R China
4.Southern Med Univ, Peoples Hosp Shilong, Dept Lab Med, Dongguan 523321, Peoples R China
5.Peking Univ, Shenzhen Hosp, Dept Lab Med, Shenzhen 518036, Peoples R China
6.Peking Univ, Shenzhen Hosp, Dept Pathol, Shenzhen 518036, Peoples R China

Recommended Citation:
Diao, Ruiying,Fok, Kin Lam,Zhao, Li,et al. Decreased expression of cystic fibrosis transmembrane conductance regulator impairs sperm quality in aged men[J]. REPRODUCTION,2013,146(6):637-645.
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