IR@PKUHSC  > 北京大学基础医学院  > 药理学系
学科主题基础医学
Endothelium-dependent vasorelaxant and antiproliferative effects of apigenin
Zhang, YH; Park, YS; Kim, TJ; Fang, LH; Ahn, HY; Hong, JT; Kim, Y; Lee, CK; Yun, YP
关键词apigenin nitric oxide cGMP vasorelaxation proliferation rat aorta
刊名GENERAL PHARMACOLOGY-THE VASCULAR SYSTEM
2000-12-01
35期:6页:341-347
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Pharmacology & Pharmacy
研究领域[WOS]Pharmacology & Pharmacy
关键词[WOS]SMOOTH-MUSCLE CELL ; PROTEIN-KINASE-C ; NITRIC-OXIDE ; METHYLENE-BLUE ; GUINEA-PIG ; FLAVONOIDS ; ATHEROSCLEROSIS ; PROLIFERATION ; INHIBITION ; RELAXATION
英文摘要

This study was designed to determine whether the relaxant effect of apigenin was endothelium dependent and to examine the possible antiproliferative effect of apigenin. Apigenin relaxed the phenylephrine-precontracted endothelium-intact aortic rings with IC50 value of 3.7+/-0.5 muM and removal of a functional endothelium significantly attenuated this relaxation (IC50 = 8.2+/-0.9 muM). However, apigenin did not affect the 0.1 muM phorbol 12,13-dibutyrate-induced contraction (IC50 = 34.6 +/- 1.2 muM) within the concentration range that relaxed the phenylephrine-contracted arteries, suggesting that apigenin did not influence protein kinase C-mediated contractile mechanisms in rat aorta. Pretreatment of apigenin significantly potentiated the relaxant effect of acetylcholine on phenylephrine-induced contraction. Pretreatment with N-G-nitro-L-arginine methyl ester (L-NAME) or methylene blue reduced the relaxant effect of apigenin. Apigenin (10 muM) increased the guanosine 3′,5′-cyclic monophosphate (cGMP) content of endothelium-intact tissues. Pretreatment with L-NAME (100 muM) or removal of endothelium significantly suppressed the effect of apigenin on cGMP production. In addition, apigenin significantly inhibited [H-3]thymidine incorporation into DNA of primary cultured rat aortic smooth muscle cell in a dose-dependent manner. These findings suggest that besides influx and release of Ca2+, nitric oxide (NO) and cGMP may account for the apigenin-induced endothelium-dependent relaxation and hypotensive activity. Both vasorelaxant and antiproliferative activities may contribute to a benefit of apigenin in the vascular system. (C) 2002 Elsevier Science Inc. All rights reserved.

语种英语
WOS记录号WOS:000177023800008
Citation statistics
Cited Times:27[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62562
Collection北京大学基础医学院_药理学系
作者单位1.Chungbuk Natl Univ, Coll Pharm, Heungduk Gu, Cheongju 361763, Chungbuk, South Korea
2.Peking Univ, Sch Basic Med Sci, Dept Pharmacol, Beijing 100083, Peoples R China
3.Chungbuk Natl Univ, Res Ctr Bioresource & Hlth, Cheongju 361763, South Korea
4.Chungbuk Natl Univ, Coll Med, Cheongju 361763, South Korea
Recommended Citation
GB/T 7714
Zhang, YH,Park, YS,Kim, TJ,et al. Endothelium-dependent vasorelaxant and antiproliferative effects of apigenin[J]. GENERAL PHARMACOLOGY-THE VASCULAR SYSTEM,2000,35(6):341-347.
APA Zhang, YH.,Park, YS.,Kim, TJ.,Fang, LH.,Ahn, HY.,...&Yun, YP.(2000).Endothelium-dependent vasorelaxant and antiproliferative effects of apigenin.GENERAL PHARMACOLOGY-THE VASCULAR SYSTEM,35(6),341-347.
MLA Zhang, YH,et al."Endothelium-dependent vasorelaxant and antiproliferative effects of apigenin".GENERAL PHARMACOLOGY-THE VASCULAR SYSTEM 35.6(2000):341-347.
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