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学科主题: 基础医学
题名:
Endothelium-dependent vasorelaxant and antiproliferative effects of apigenin
作者: Zhang, YH; Park, YS; Kim, TJ; Fang, LH; Ahn, HY; Hong, JT; Kim, Y; Lee, CK; Yun, YP
关键词: apigenin ; nitric oxide ; cGMP ; vasorelaxation ; proliferation ; rat aorta
刊名: GENERAL PHARMACOLOGY-THE VASCULAR SYSTEM
发表日期: 2000-12-01
卷: 35, 期:6, 页:341-347
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pharmacology & Pharmacy
研究领域[WOS]: Pharmacology & Pharmacy
关键词[WOS]: SMOOTH-MUSCLE CELL ; PROTEIN-KINASE-C ; NITRIC-OXIDE ; METHYLENE-BLUE ; GUINEA-PIG ; FLAVONOIDS ; ATHEROSCLEROSIS ; PROLIFERATION ; INHIBITION ; RELAXATION
英文摘要:

This study was designed to determine whether the relaxant effect of apigenin was endothelium dependent and to examine the possible antiproliferative effect of apigenin. Apigenin relaxed the phenylephrine-precontracted endothelium-intact aortic rings with IC50 value of 3.7+/-0.5 muM and removal of a functional endothelium significantly attenuated this relaxation (IC50 = 8.2+/-0.9 muM). However, apigenin did not affect the 0.1 muM phorbol 12,13-dibutyrate-induced contraction (IC50 = 34.6 +/- 1.2 muM) within the concentration range that relaxed the phenylephrine-contracted arteries, suggesting that apigenin did not influence protein kinase C-mediated contractile mechanisms in rat aorta. Pretreatment of apigenin significantly potentiated the relaxant effect of acetylcholine on phenylephrine-induced contraction. Pretreatment with N-G-nitro-L-arginine methyl ester (L-NAME) or methylene blue reduced the relaxant effect of apigenin. Apigenin (10 muM) increased the guanosine 3′,5′-cyclic monophosphate (cGMP) content of endothelium-intact tissues. Pretreatment with L-NAME (100 muM) or removal of endothelium significantly suppressed the effect of apigenin on cGMP production. In addition, apigenin significantly inhibited [H-3]thymidine incorporation into DNA of primary cultured rat aortic smooth muscle cell in a dose-dependent manner. These findings suggest that besides influx and release of Ca2+, nitric oxide (NO) and cGMP may account for the apigenin-induced endothelium-dependent relaxation and hypotensive activity. Both vasorelaxant and antiproliferative activities may contribute to a benefit of apigenin in the vascular system. (C) 2002 Elsevier Science Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000177023800008
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62562
Appears in Collections:基础医学院_药理学系_期刊论文

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作者单位: 1.Chungbuk Natl Univ, Coll Pharm, Heungduk Gu, Cheongju 361763, Chungbuk, South Korea
2.Peking Univ, Sch Basic Med Sci, Dept Pharmacol, Beijing 100083, Peoples R China
3.Chungbuk Natl Univ, Res Ctr Bioresource & Hlth, Cheongju 361763, South Korea
4.Chungbuk Natl Univ, Coll Med, Cheongju 361763, South Korea

Recommended Citation:
Zhang, YH,Park, YS,Kim, TJ,et al. Endothelium-dependent vasorelaxant and antiproliferative effects of apigenin[J]. GENERAL PHARMACOLOGY-THE VASCULAR SYSTEM,2000,35(6):341-347.
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