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学科主题: 临床医学
题名:
Expression and functional analysis of beta-adrenoceptor subtypes in rabbit submandibular gland
作者: Li, Yu-Ming; Zhang, Yan; Xiang, Bin; Zhang, You-Yi; Wu, Li-Ling; Yu, Guang-Yan
关键词: beta-adrenoceptor subtypes ; saliva secretion ; rabbit ; submandibular gland
刊名: LIFE SCIENCES
发表日期: 2006-10-26
DOI: 10.1016/j.lfs.2006.07.017
卷: 79, 期:22, 页:2091-2098
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, Research & Experimental ; Pharmacology & Pharmacy
研究领域[WOS]: Research & Experimental Medicine ; Pharmacology & Pharmacy
关键词[WOS]: ADRENERGIC-RECEPTOR SUBTYPES ; SALIVARY-GLAND ; MOLECULAR-CLONING ; DOWN-REGULATION ; MESSENGER-RNA ; GROWTH-FACTOR ; RAT ; SECRETION ; PROTEINS ; CELLS
英文摘要:

beta-Adrenoceptors (beta-ARs) mediate important physiological functions in salivary glands. Here we investigated the expression and function of beta-AR subtypes in rabbit submandibular gland (SMG). Both beta(1)- and beta(2)-ARs, but not beta(3)-AR, were strongly expressed in rabbit SMG beta(1)-AR proteins were widely expressed in acinar and ductal cells whereas beta(2)-AR proteins were mainly detected in ductal cells. A [H-3]-dihydroalprenolol binding assay revealed that beta(1)-AR B-max was 186 +/- 11.9 frnol/mg protein and K-d was 2.71 +/- 0.23 nM. A competitive binding assay with CGP 20712A. a beta(1)-AR antagonist, indicated that the proportion of beta(1)-AR and beta(2)-AR was 71.9% and 28.1%, respectively. Gland perfusion with the beta-AR agonist isoproterenol induced a significant increase in saliva secretion which was abolished by pretreatment with the non-selective beta-AR antagonist propranolol. Pretreatment with beta(1)- or beta(2)-AR selective antagonists, CGP 20712A or ICI 118551, diminished isoproterenol-induced increase in saliva secretion by 71.2% and 28.8%, respectively. The expression of alpha-amylase mRNA was significantly stimulated by isoproterenol. which was eliminated by propranolol and CGP 20712A. Perfusion with isoproterenol decreased alpha-amylase protein storage in SMG and increased alpha-amylase activity in saliva. These alterations became less significant after pretreatment with propranolol and CGP 20712A. Our results suggest that both beta(1)- and beta(2)-ARs are expressed in rabbit SMG. beta(1)-AR is the predominant subtype and may play an important role in regulating saliva and alpha-amylase secretion. (c) 2006 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000241708200004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62566
Appears in Collections:北京大学第三临床医学院_心血管内科_期刊论文

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作者单位: 1.Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing 100083, Peoples R China
2.Peking Univ Sch, Dept Oral & Maxillofacial Surg, Beijing 100081, Peoples R China
3.Peking Univ Sch, Sch & Hosp Stomatol, Beijing 100081, Peoples R China
4.Peking Univ, Hlth Sci Ctr, Dept Physiol & Pathophysiol, Beijing 100083, Peoples R China
5.Peking Univ, Inst Vasc Med, Hosp 3, Beijing 100083, Peoples R China

Recommended Citation:
Li, Yu-Ming,Zhang, Yan,Xiang, Bin,et al. Expression and functional analysis of beta-adrenoceptor subtypes in rabbit submandibular gland[J]. LIFE SCIENCES,2006,79(22):2091-2098.
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