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Human TFDP3, a novel DP protein, inhibits DNA binding and transactivation by E2F
Qiao, Huan; Di Stefano, Luisa; Tian, Chan; Li, Yun-Yan; Yin, Yan-Hui; Qian, Xiao-Ping; Pang, Xue-Wen; Li, Yan; McNutt, Michael Allen; Helin, Kristian; Zhang, Yu; Chen, Wei-Feng
刊名JOURNAL OF BIOLOGICAL CHEMISTRY
2007-01-05
DOI10.1074/jbc.M606169200
282期:1页:454-466
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]TRANSCRIPTION FACTOR DRTF1/E2F ; THYMIC NEGATIVE SELECTION ; CELL-CYCLE PROGRESSION ; FAMILY-MEMBER ; GROWTH SUPPRESSION ; APOPTOSIS ; PROLIFERATION ; EXPRESSION ; INDUCTION ; REPRESSOR
英文摘要

The two known DP proteins, TFDP1 and -2, bind E2Fs to form heterodimers essential for high affinity DNA binding and efficient transcriptional activation/repression. Here we report the identification of a new member of the DP family, human TFDP3. Despite the high degree of sequence similarity, TFDP3 is apparently distinct from TFDP1 in function. Although TFDP3 retained the capacity to bind to E2F proteins, the resulting heterodimers failed to interact with the E2F consensus sequence. In contrast to the stimulatory effect of TFDP1, TFDP3 inhibited E2F-mediated transcriptional activation. Consistent with this observation, we found that ectopic expression of TFDP3 impaired cell cycle progression from G(1) to S phase instead of facilitating such a transition as TFDP1 does. Sequence substitution analysis indicated that the DNA binding domain of TFDP3 was primarily responsible for the lack of DNA binding ability of E2F-TFDP3 heterodimers and the inhibition of E2F-mediated transcriptional activation. Fine mapping further revealed four amino acids in this region, which were critical for the functional conversion from activation by TFDP1 to suppression by TFDP3. In conclusion, these studies identify a new DP protein and a novel mechanism whereby E2F function is regulated.

语种英语
WOS记录号WOS:000243166500051
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被引频次:16[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62581
专题基础医学院_免疫学系
作者单位1.Peking Univ, Hlth Sci Ctr, Dept Immunol, Beijing 100083, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Pathol, Beijing 100083, Peoples R China
3.European Inst Oncol, Dept Expt Oncol, I-20141 Milan, Italy
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Qiao, Huan,Di Stefano, Luisa,Tian, Chan,et al. Human TFDP3, a novel DP protein, inhibits DNA binding and transactivation by E2F[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2007,282(1):454-466.
APA Qiao, Huan.,Di Stefano, Luisa.,Tian, Chan.,Li, Yun-Yan.,Yin, Yan-Hui.,...&Chen, Wei-Feng.(2007).Human TFDP3, a novel DP protein, inhibits DNA binding and transactivation by E2F.JOURNAL OF BIOLOGICAL CHEMISTRY,282(1),454-466.
MLA Qiao, Huan,et al."Human TFDP3, a novel DP protein, inhibits DNA binding and transactivation by E2F".JOURNAL OF BIOLOGICAL CHEMISTRY 282.1(2007):454-466.
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