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学科主题: 临床医学
题名:
Identification of Hsf1 as a Novel Androgen Receptor-Regulated Gene in Mouse Sertoli Cells
作者: Yang, Lihua1,2; Wang, Yadong1,3; Zhang, Qiang1; Lai, Yongqing1; Li, Cailing1; Zhang, Qiaoxia2; Huang, Weiren2; Duan, Yonggang2; Jiang, Zhimao1; Li, Xianxin1; Cai, Zhiming2; Mou, Lisha2; Gui, Yaoting1
刊名: MOLECULAR REPRODUCTION AND DEVELOPMENT
发表日期: 2014-06-01
DOI: 10.1002/mrd.22318
卷: 81, 期:6, 页:514-523
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Cell Biology ; Developmental Biology ; Reproductive Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Cell Biology ; Developmental Biology ; Reproductive Biology
关键词[WOS]: SHOCK TRANSCRIPTION FACTOR-1 ; MALE GERM-CELLS ; HOMEOBOX GENE ; SELECTIVE KNOCKOUT ; RESPONSE ELEMENTS ; PROSTATE-CANCER ; MICE LACKING ; SPERMATOGENESIS ; EXPRESSION ; PROTEIN
英文摘要:

Androgen signaling plays a crucial role in spermatogenesis, yet few downstream targets for this signaling pathway have been identified. In the current study, we found that the expression of heat-shock transcription factor 1 (Hsf1) was increased in the testes of Sertoli cell-selective androgen receptor knockout (S-AR(-/y)) mice compared with wild-type mice by quantitative real-time PCR, and the expression of HSF1 in the S-AR(-/y) Sertoli cells was significantly increased, based on immunofluorescence analysis. In vitro cell-culture studies showed that testosterone repressed the expression of Hsf1 in TM4 cells, a mouse Sertoli cell line. Moreover, a luciferase assay, electrophoretic mobility shift assay, and chromatin immunoprecipitation assay showed that testosterone repressed Hsf1 expression by facilitating the binding of androgen receptor to the Hsf1 promoter. Our experiments also demonstrated that testosterone-mediated inhibition of Hsf1 transcription down-regulated the expression of heat-shock proteins HSP105 and HSP60. Taken together, these results reveal that Hsf1 is a novel target of androgen receptor in mouse Sertoli cells, and testosterone and its receptor regulate the process of spermatogenesis partially by inhibiting Hsf1 expression. (C) 2014 Wiley Periodicals, Inc.

语种: 英语
所属项目编号: 2011CB944303 ; 31271244 ; 81200465 ; CXB201104220045A
项目资助者: National Key Scientific Program of China ; National Nature Science Foundation of China ; Shenzhen Foundation of Science and Technology
WOS记录号: WOS:000337625800005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62589
Appears in Collections:北京大学深圳医院_期刊论文

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作者单位: 1.Fifth Affiliated Hosp, Zunyi Med Coll, Zhuhai, Peoples R China
2.Peking Univ, Shenzhen Hosp, Guangdong & Shenzhen Key Lab Male Reprod Med & Ge, Inst Urol,Shenzhen PKU HKUST Med Ctr, Shenzhen 518036, Peoples R China
3.Shenzhen Univ, Affiliated Hosp 1, Shenzhen Peoples Hosp 2, Dept Urol Surg, Shenzhen 518035, Peoples R China

Recommended Citation:
Yang, Lihua,Wang, Yadong,Zhang, Qiang,et al. Identification of Hsf1 as a Novel Androgen Receptor-Regulated Gene in Mouse Sertoli Cells[J]. MOLECULAR REPRODUCTION AND DEVELOPMENT,2014,81(6):514-523.
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