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Identification of Hsf1 as a Novel Androgen Receptor-Regulated Gene in Mouse Sertoli Cells
Yang, Lihua1,2; Wang, Yadong1,3; Zhang, Qiang1; Lai, Yongqing1; Li, Cailing1; Zhang, Qiaoxia2; Huang, Weiren2; Duan, Yonggang2; Jiang, Zhimao1; Li, Xianxin1; Cai, Zhiming2; Mou, Lisha2; Gui, Yaoting1
刊名MOLECULAR REPRODUCTION AND DEVELOPMENT
2014-06-01
DOI10.1002/mrd.22318
81期:6页:514-523
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Cell Biology ; Developmental Biology ; Reproductive Biology
资助者National Key Scientific Program of China ; National Nature Science Foundation of China ; Shenzhen Foundation of Science and Technology ; National Key Scientific Program of China ; National Nature Science Foundation of China ; Shenzhen Foundation of Science and Technology
研究领域[WOS]Biochemistry & Molecular Biology ; Cell Biology ; Developmental Biology ; Reproductive Biology
关键词[WOS]SHOCK TRANSCRIPTION FACTOR-1 ; MALE GERM-CELLS ; HOMEOBOX GENE ; SELECTIVE KNOCKOUT ; RESPONSE ELEMENTS ; PROSTATE-CANCER ; MICE LACKING ; SPERMATOGENESIS ; EXPRESSION ; PROTEIN
英文摘要

Androgen signaling plays a crucial role in spermatogenesis, yet few downstream targets for this signaling pathway have been identified. In the current study, we found that the expression of heat-shock transcription factor 1 (Hsf1) was increased in the testes of Sertoli cell-selective androgen receptor knockout (S-AR(-/y)) mice compared with wild-type mice by quantitative real-time PCR, and the expression of HSF1 in the S-AR(-/y) Sertoli cells was significantly increased, based on immunofluorescence analysis. In vitro cell-culture studies showed that testosterone repressed the expression of Hsf1 in TM4 cells, a mouse Sertoli cell line. Moreover, a luciferase assay, electrophoretic mobility shift assay, and chromatin immunoprecipitation assay showed that testosterone repressed Hsf1 expression by facilitating the binding of androgen receptor to the Hsf1 promoter. Our experiments also demonstrated that testosterone-mediated inhibition of Hsf1 transcription down-regulated the expression of heat-shock proteins HSP105 and HSP60. Taken together, these results reveal that Hsf1 is a novel target of androgen receptor in mouse Sertoli cells, and testosterone and its receptor regulate the process of spermatogenesis partially by inhibiting Hsf1 expression. (C) 2014 Wiley Periodicals, Inc.

语种英语
所属项目编号2011CB944303 ; 31271244 ; 81200465 ; CXB201104220045A
资助者National Key Scientific Program of China ; National Nature Science Foundation of China ; Shenzhen Foundation of Science and Technology ; National Key Scientific Program of China ; National Nature Science Foundation of China ; Shenzhen Foundation of Science and Technology
WOS记录号WOS:000337625800005
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62589
专题北京大学深圳医院
作者单位1.Fifth Affiliated Hosp, Zunyi Med Coll, Zhuhai, Peoples R China
2.Peking Univ, Shenzhen Hosp, Guangdong & Shenzhen Key Lab Male Reprod Med & Ge, Inst Urol,Shenzhen PKU HKUST Med Ctr, Shenzhen 518036, Peoples R China
3.Shenzhen Univ, Affiliated Hosp 1, Shenzhen Peoples Hosp 2, Dept Urol Surg, Shenzhen 518035, Peoples R China
推荐引用方式
GB/T 7714
Yang, Lihua,Wang, Yadong,Zhang, Qiang,et al. Identification of Hsf1 as a Novel Androgen Receptor-Regulated Gene in Mouse Sertoli Cells[J]. MOLECULAR REPRODUCTION AND DEVELOPMENT,2014,81(6):514-523.
APA Yang, Lihua.,Wang, Yadong.,Zhang, Qiang.,Lai, Yongqing.,Li, Cailing.,...&Gui, Yaoting.(2014).Identification of Hsf1 as a Novel Androgen Receptor-Regulated Gene in Mouse Sertoli Cells.MOLECULAR REPRODUCTION AND DEVELOPMENT,81(6),514-523.
MLA Yang, Lihua,et al."Identification of Hsf1 as a Novel Androgen Receptor-Regulated Gene in Mouse Sertoli Cells".MOLECULAR REPRODUCTION AND DEVELOPMENT 81.6(2014):514-523.
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