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学科主题: 基础医学
题名:
NAT10, a nucleolar protein, localizes to the midbody and regulates cytokinesis and acetylation of microtubules
作者: Shen, Qi; Zheng, Xingzheng; McNutt, Michael A.; Guang, Lizhao; Sun, Ying; Wang, Jiaochen; Gong, Yilei; Hou, Lin; Zhang, Bo
关键词: Histone acetyltransferase ; Nucleolus ; Midbody ; Cytokinesis ; DNA damage
刊名: EXPERIMENTAL CELL RESEARCH
发表日期: 2009-06-10
DOI: 10.1016/j.yexcr.2009.03.007
卷: 315, 期:10, 页:1653-1667
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology ; Cell Biology
研究领域[WOS]: Oncology ; Cell Biology
关键词[WOS]: TRANSCRIPTIONAL ACTIVATION ; MITOTIC CHROMOSOMES ; CO-LOCALIZE ; NUCLEAR ; MITOSIS ; RELOCALIZATION ; CENTROSOMES ; DYNAMICS ; CANCER
英文摘要:

The midbody is a structural organelle formed in late phase mitosis which is responsible for completion of cytokinesis. Although various kinds of proteins have been found to distribute or immigrate to this organelle, their functions have still not been completely worked out. In this study, we demonstrated that NAT10 (N-acetyltransferase 10, NAT10) is not only predominantly distributed in the nucleolus in interphase, but is also concentrated in the mitotic midbody during telophase. The domain in N-terminal residues 549-834 of NAT10 specifically mediated its subcellular localization. Treatment with genotoxic agents or irradiation increased concentration of NAT10 in both the nucleolus and midbody. Moreover, DNA damage induced increase of NAT10 in the midbody apparently accompanied by in situ elevation of the level of acetylated alpha-tubulin, suggesting that it plays a role in maintaining or enhancing stability of alpha-tubulin. The depletion of NAT10 induced defects in nucleolar assembly, cytokinesis and decreased acetylated alpha-tubulin, leading to G2/M cell cycle arrest or delay of mitotic exit. In addition, over-expression of NAT10 was found in a variety of soft tissue sarcomas, and correlated with tumor histological grading. These results indicate that NAT10 may play an important role in cell division through facilitating reformation of the nucleolus and midbody in the late phase of cell mitosis, and stabilization of microtubules. (C) 2009 Elsevier Inc. All rights reserved.

语种: 英语
所属项目编号: 30770830 ; 200800010060
项目资助者: National Natural Science Foundation of China ; Chinese Education Ministry
WOS记录号: WOS:000266281000003
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62597
Appears in Collections:基础医学院_病理学系_期刊论文

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作者单位: Peking Univ, Hlth Sci Ctr, Dept Pathol, Beijing 100191, Peoples R China

Recommended Citation:
Shen, Qi,Zheng, Xingzheng,McNutt, Michael A.,et al. NAT10, a nucleolar protein, localizes to the midbody and regulates cytokinesis and acetylation of microtubules[J]. EXPERIMENTAL CELL RESEARCH,2009,315(10):1653-1667.
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