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学科主题临床医学
Afyap1, encoding a bZip transcriptional factor of Aspergillus fumigatus, contributes to oxidative stress response but is not essential to the virulence of this pathogen in mice immunosuppressed by cyclophosphamide and triamcinolone
Qiao, Jianjun1,2; Kontoyiannis, Dimitrios P.3; Calderone, Richard4; Li, Dongmei4; Ma, Yan1,2; Wan, Zhe1,2; Li, Ruoyu1,2; Liu, Wei1,2
关键词Aspergillus fumigatus Afyap1 oxidative stress virulence
刊名MEDICAL MYCOLOGY
2008
DOI10.1080/13693780802054215
46期:8页:773-782
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Mycology ; Veterinary Sciences
资助者Chinese Ministry of Education ; National Natural Science Foundation of China ; International Center for Advanced Study in the Health Sciences ; National Institute of Allergy and Infectious Disease ; Chinese Ministry of Education ; National Natural Science Foundation of China ; International Center for Advanced Study in the Health Sciences ; National Institute of Allergy and Infectious Disease
研究领域[WOS]Mycology ; Veterinary Sciences
关键词[WOS]SACCHAROMYCES-CEREVISIAE ; MULTIDRUG-RESISTANCE ; CANDIDA-ALBICANS ; AGROBACTERIUM-TUMEFACIENS ; INSERTIONAL MUTAGENESIS ; MEDIATED TRANSFORMATION ; INVASIVE ASPERGILLOSIS ; NUCLEAR EXPORT ; EFFICIENT TOOL ; GENE
英文摘要

Aspergillus fumigatus, an important human fungal pathogen, encounters high levels of reactive oxygen species following its ingestion by phagocytes. Reactive oxygen species are important mediators of the fungicidal activities of phagocytes. In yeasts, YAP1 encodes for transcriptional factors that contribute to their oxidative stress response and given the importance of the stress response, we hypothesized that the YAP1 homologue in A. fumigatus plays a similar role in this fungus. In this study, we found that Afyap1, the Yap1 homologue of A. fumigatus, confers protection against oxidative stress. Replacement of Afyap1 with the marker gene pyrG (Afyap1) resulted in hypersensitivity of A. fumigatus to oxidants such as H2O2 and menadione. In contrast, an A. fumigatus strain harboring multiple-copy Afyap1 was resistant to these two oxidants as well as the oxidant diamide. However, Afyap1 and strain harboring multiple-copy Afyap1 were comparable in their virulence to a wild-type A. fumigatus strain in a murine model of invasive pulmonary aspergillosis. Taken together, these results demonstrate that Afyap1 is involved in oxidative stress response but is not an essential virulence factor for A. fumigatus.

语种英语
所属项目编号107002 ; 30500027 ; TW005926 ; U01 AI 48830
资助者Chinese Ministry of Education ; National Natural Science Foundation of China ; International Center for Advanced Study in the Health Sciences ; National Institute of Allergy and Infectious Disease ; Chinese Ministry of Education ; National Natural Science Foundation of China ; International Center for Advanced Study in the Health Sciences ; National Institute of Allergy and Infectious Disease
WOS记录号WOS:000260680700002
引用统计
被引频次:27[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62600
专题北京大学第一临床医学院_皮肤性病科
作者单位1.Peking Univ, Res Ctr Med Mycol, Beijing 100871, Peoples R China
2.Peking Univ, Hosp 1, Dept Dermatol, Beijing 100871, Peoples R China
3.Univ Texas MD Anderson Canc Ctr, Dept Infect Dis Infect Control & Employee Hlth, Houston, TX 77030 USA
4.Georgetown Univ, Sch Med, Dept Microbiol & Immunol, Washington, DC USA
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Qiao, Jianjun,Kontoyiannis, Dimitrios P.,Calderone, Richard,et al. Afyap1, encoding a bZip transcriptional factor of Aspergillus fumigatus, contributes to oxidative stress response but is not essential to the virulence of this pathogen in mice immunosuppressed by cyclophosphamide and triamcinolone[J]. MEDICAL MYCOLOGY,2008,46(8):773-782.
APA Qiao, Jianjun.,Kontoyiannis, Dimitrios P..,Calderone, Richard.,Li, Dongmei.,Ma, Yan.,...&Liu, Wei.(2008).Afyap1, encoding a bZip transcriptional factor of Aspergillus fumigatus, contributes to oxidative stress response but is not essential to the virulence of this pathogen in mice immunosuppressed by cyclophosphamide and triamcinolone.MEDICAL MYCOLOGY,46(8),773-782.
MLA Qiao, Jianjun,et al."Afyap1, encoding a bZip transcriptional factor of Aspergillus fumigatus, contributes to oxidative stress response but is not essential to the virulence of this pathogen in mice immunosuppressed by cyclophosphamide and triamcinolone".MEDICAL MYCOLOGY 46.8(2008):773-782.
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