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学科主题: 临床医学
题名:
Isolation and identification of a protein interacting with P37 protein of Mycoplasma hyorhinis
作者: Sun, YN; Jin, GL; Zhang, JZ; Wu, J; Shou, CC
关键词: M. hyorhinis ; yeast two-hybrid ; P37 ; Norpeg
刊名: PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
发表日期: 2004-10-01
卷: 31, 期:10, 页:902-906
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
英文摘要:

High ratio infection of Mycoplasma hyorhinis in gastric tumor tissues suggests a possible association between mycoplasma infection and cancer. P37, an outer-membrane bacterial protein from Mycoplasma hyorhinis, increases neoplastic invasivity and metastasis and induces TNF-alpha secretion from human peripheral blood mononuclear cells (PBMC). To investigate the functional mechanism of P37, yeast two-hybrid system was used to isolate proteins interacted with P37 from a human placenta cDNA library. Among the 2.6 X 10(6) transformant clones, one positive clone was obtained. Sequence analysis revealed that the clone is a cDNA fragment from Norpeg and encode a carboxy terminal protein. The interaction between P37 and Norpeg was further confirmed by ELISA and GST-Pull down assay. This result would be useful to study the function of P37 further.

语种: 中文
WOS记录号: WOS:000224887800008
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62607
Appears in Collections:北京大学临床肿瘤学院_期刊论文

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作者单位: Peking Univ, Sch Oncol, Beijing Inst Canc Res, Beijing 100034, Peoples R China

Recommended Citation:
Sun, YN,Jin, GL,Zhang, JZ,et al. Isolation and identification of a protein interacting with P37 protein of Mycoplasma hyorhinis[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2004,31(10):902-906.
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