IR@PKUHSC  > 北京大学临床肿瘤学院
学科主题临床医学
Isolation and identification of a protein interacting with P37 protein of Mycoplasma hyorhinis
Sun, YN; Jin, GL; Zhang, JZ; Wu, J; Shou, CC
关键词M. hyorhinis yeast two-hybrid P37 Norpeg
刊名PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
2004-10-01
31期:10页:902-906
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
英文摘要

High ratio infection of Mycoplasma hyorhinis in gastric tumor tissues suggests a possible association between mycoplasma infection and cancer. P37, an outer-membrane bacterial protein from Mycoplasma hyorhinis, increases neoplastic invasivity and metastasis and induces TNF-alpha secretion from human peripheral blood mononuclear cells (PBMC). To investigate the functional mechanism of P37, yeast two-hybrid system was used to isolate proteins interacted with P37 from a human placenta cDNA library. Among the 2.6 X 10(6) transformant clones, one positive clone was obtained. Sequence analysis revealed that the clone is a cDNA fragment from Norpeg and encode a carboxy terminal protein. The interaction between P37 and Norpeg was further confirmed by ELISA and GST-Pull down assay. This result would be useful to study the function of P37 further.

语种中文
WOS记录号WOS:000224887800008
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62607
Collection北京大学临床肿瘤学院
作者单位Peking Univ, Sch Oncol, Beijing Inst Canc Res, Beijing 100034, Peoples R China
Recommended Citation
GB/T 7714
Sun, YN,Jin, GL,Zhang, JZ,et al. Isolation and identification of a protein interacting with P37 protein of Mycoplasma hyorhinis[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2004,31(10):902-906.
APA Sun, YN,Jin, GL,Zhang, JZ,Wu, J,&Shou, CC.(2004).Isolation and identification of a protein interacting with P37 protein of Mycoplasma hyorhinis.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,31(10),902-906.
MLA Sun, YN,et al."Isolation and identification of a protein interacting with P37 protein of Mycoplasma hyorhinis".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 31.10(2004):902-906.
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