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学科主题: 临床医学
题名:
microRNA-320a inhibits tumor invasion by targeting neuropilin 1 and is associated with liver metastasis in colorectal cancer
作者: Zhang, Yujun1; He, Xiangjun1; Liu, Yulan1,2; Ye, Yingjiang3; Zhang, Hui3; He, Peiying1; Zhang, Qi1; Dong, Lingyi3; Liu, Yujing1; Dong, Jianqiang1
关键词: microRNAs ; colorectal cancer ; liver metastasis ; neuropilin 1
刊名: ONCOLOGY REPORTS
发表日期: 2012-03-01
DOI: 10.3892/or.2011.1561
卷: 27, 期:3, 页:685-694
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: BREAST-CANCER ; COLON-CANCER ; GROWTH-FACTOR ; E-CADHERIN ; EXPRESSION ; GENES ; FAMILY ; CELLS ; PCR ; IDENTIFICATION
英文摘要:

MicroRNAs (miRNAs) have been implicated in regulating diverse cellular pathways. Although there is emerging evidence that various miRNAs function as oncogenes or tumor suppressors in colorectal cancer (CRC), the role of miRNAs in mediating liver metastasis remains unexplored. The expression profile of miRNAs in liver metastasis and primary CRC tissues was analyzed by miRNA microarrays and verified by real-time polymerase chain reaction (PCR). In 62 CRC patients, the expression levels of miR-320a were determined by real-time PCR, and the effects on migration and invasion of miR-320a were determined using a transwell assay. miR-320a target genes were confirmed by luciferase assay, real-time PCR and Western blot analysis. A set of miRNAs was found to be dysregulated in the liver metastasis tissues compared to matched primary CRC tissues, and the expression levels of miR-320a were significantly decreased in the liver metastasis tissues examined. miR-320a was correlated with tumor progression in CRC. miR-320a was downregulated in liver metastatic colon cancer cells and inhibited liver metastatic colon cancer cell migration and invasion. miR-320a directly binds to the 3′UTR of neuropilin 1 (NRP-1), a protein that functions as a co-receptor of vascular epithelial growth factor. miR-320a downregulated the expression of NRP-1 at both the mRNA and protein levels. These data demonstrated that miR-320a may be useful for identifying CRC patients that are at an elevated risk for developing liver metastasis. Our findings suggest that miR-320a may be a novel therapeutic candidate for the treatment of colorectal cancer.

语种: 英语
所属项目编号: 31140001 ; RDB2008-04 ; 20090001120084
项目资助者: National Natural Science Foundation of China ; Peking University People&prime ; s Hospital ; Ministry of Education of China
WOS记录号: WOS:000300118700012
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62614
Appears in Collections:北京大学第二临床医学院_临床分子生物学研究所_期刊论文

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作者单位: 1.Peking Univ, Peoples Hosp, Inst Clin Mol Biol, Beijing 100044, Peoples R China
2.Peking Univ, Peoples Hosp, Dept Gastroenterol, Beijing 100044, Peoples R China
3.Peking Univ, Peoples Hosp, Dept Gen Surg, Beijing 100044, Peoples R China

Recommended Citation:
Zhang, Yujun,He, Xiangjun,Liu, Yulan,et al. microRNA-320a inhibits tumor invasion by targeting neuropilin 1 and is associated with liver metastasis in colorectal cancer[J]. ONCOLOGY REPORTS,2012,27(3):685-694.
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