学科主题临床医学
microRNA-320a inhibits tumor invasion by targeting neuropilin 1 and is associated with liver metastasis in colorectal cancer
Zhang, Yujun1; He, Xiangjun1; Liu, Yulan1,2; Ye, Yingjiang3; Zhang, Hui3; He, Peiying1; Zhang, Qi1; Dong, Lingyi3; Liu, Yujing1; Dong, Jianqiang1
关键词microRNAs colorectal cancer liver metastasis neuropilin 1
刊名ONCOLOGY REPORTS
2012-03-01
DOI10.3892/or.2011.1561
27期:3页:685-694
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]BREAST-CANCER ; COLON-CANCER ; GROWTH-FACTOR ; E-CADHERIN ; EXPRESSION ; GENES ; FAMILY ; CELLS ; PCR ; IDENTIFICATION
英文摘要

MicroRNAs (miRNAs) have been implicated in regulating diverse cellular pathways. Although there is emerging evidence that various miRNAs function as oncogenes or tumor suppressors in colorectal cancer (CRC), the role of miRNAs in mediating liver metastasis remains unexplored. The expression profile of miRNAs in liver metastasis and primary CRC tissues was analyzed by miRNA microarrays and verified by real-time polymerase chain reaction (PCR). In 62 CRC patients, the expression levels of miR-320a were determined by real-time PCR, and the effects on migration and invasion of miR-320a were determined using a transwell assay. miR-320a target genes were confirmed by luciferase assay, real-time PCR and Western blot analysis. A set of miRNAs was found to be dysregulated in the liver metastasis tissues compared to matched primary CRC tissues, and the expression levels of miR-320a were significantly decreased in the liver metastasis tissues examined. miR-320a was correlated with tumor progression in CRC. miR-320a was downregulated in liver metastatic colon cancer cells and inhibited liver metastatic colon cancer cell migration and invasion. miR-320a directly binds to the 3′UTR of neuropilin 1 (NRP-1), a protein that functions as a co-receptor of vascular epithelial growth factor. miR-320a downregulated the expression of NRP-1 at both the mRNA and protein levels. These data demonstrated that miR-320a may be useful for identifying CRC patients that are at an elevated risk for developing liver metastasis. Our findings suggest that miR-320a may be a novel therapeutic candidate for the treatment of colorectal cancer.

语种英语
WOS记录号WOS:000300118700012
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被引频次:74[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
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条目标识符http://ir.bjmu.edu.cn/handle/400002259/62614
专题北京大学第二临床医学院_临床分子生物学研究所
北京大学第二临床医学院_中心实验室
作者单位1.Peking Univ, Peoples Hosp, Inst Clin Mol Biol, Beijing 100044, Peoples R China
2.Peking Univ, Peoples Hosp, Dept Gastroenterol, Beijing 100044, Peoples R China
3.Peking Univ, Peoples Hosp, Dept Gen Surg, Beijing 100044, Peoples R China
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Zhang, Yujun,He, Xiangjun,Liu, Yulan,et al. microRNA-320a inhibits tumor invasion by targeting neuropilin 1 and is associated with liver metastasis in colorectal cancer[J]. ONCOLOGY REPORTS,2012,27(3):685-694.
APA Zhang, Yujun.,He, Xiangjun.,Liu, Yulan.,Ye, Yingjiang.,Zhang, Hui.,...&Dong, Jianqiang.(2012).microRNA-320a inhibits tumor invasion by targeting neuropilin 1 and is associated with liver metastasis in colorectal cancer.ONCOLOGY REPORTS,27(3),685-694.
MLA Zhang, Yujun,et al."microRNA-320a inhibits tumor invasion by targeting neuropilin 1 and is associated with liver metastasis in colorectal cancer".ONCOLOGY REPORTS 27.3(2012):685-694.
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