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Expression of an ovarian cancer anti-idiotype antibody (6B11VLVHCH3) in chinese hamster ovary (CHO) cells with improved immunoactivity and stability over proteins expressed in prokaryotic cells
Shi, Jlngjlng; Chang, Xiaohong; Feng, He; Cheng, Yexia; Cheng, Hongyan; Guo, Huifang; Ye, Xue; Cui, Heng
刊名HYBRIDOMA
2007-10-01
DOI10.1089/hyb.2007.0511
26期:5页:289-295
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Immunology
研究领域[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Immunology
关键词[WOS]MONOCLONAL-ANTIBODY ; CARCINOMA ; CRYSTALLIZATION ; GLYCOSYLATION ; DOMAINS
英文摘要

The 6B11VLVHCH3 is an anti-idiotype antibody to ovarian cancer. It can mimic the ovarian cancer-associated antigen OC166-9. We have previously expressed it in Escherichia coli. We now express the 6B11VLVHCH3 in a Chinese hamster ovary (CHO) cell system. The 6B11VLVHhCH3 sequence was amplified from the prokaryotic vector and cloned into the eukaryotic expression vector pSecTag2. The construct was transfected into CHO cells. Then the transfected monoclonal CHO cells were sequentially adapted to growth in serum-free medium. Functional differences between the antibody produced in E. coli or CHO cells were assessed. The antibody produced by CHO cells was able to both mimic ovarian cancer antigen and retain FC region functionality. Importantly, the antigen mimicry activity of 6B11VLVHhCH3 produced by CHO cells is approximately 20 times higher than antibody expressed by E. coli. This method of expression provides a safer and higher quality antibody for clinical drug development.

语种英语
WOS记录号WOS:000250655200002
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62624
专题北京大学第二临床医学院_妇科肿瘤中心
作者单位Peking Univ, Peoples Hosp, Gynecol Oncol Ctr, Beijing 100044, Peoples R China
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GB/T 7714
Shi, Jlngjlng,Chang, Xiaohong,Feng, He,et al. Expression of an ovarian cancer anti-idiotype antibody (6B11VLVHCH3) in chinese hamster ovary (CHO) cells with improved immunoactivity and stability over proteins expressed in prokaryotic cells[J]. HYBRIDOMA,2007,26(5):289-295.
APA Shi, Jlngjlng.,Chang, Xiaohong.,Feng, He.,Cheng, Yexia.,Cheng, Hongyan.,...&Cui, Heng.(2007).Expression of an ovarian cancer anti-idiotype antibody (6B11VLVHCH3) in chinese hamster ovary (CHO) cells with improved immunoactivity and stability over proteins expressed in prokaryotic cells.HYBRIDOMA,26(5),289-295.
MLA Shi, Jlngjlng,et al."Expression of an ovarian cancer anti-idiotype antibody (6B11VLVHCH3) in chinese hamster ovary (CHO) cells with improved immunoactivity and stability over proteins expressed in prokaryotic cells".HYBRIDOMA 26.5(2007):289-295.
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