北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第二临床医学院  > 妇科肿瘤中心  > 期刊论文
学科主题: 临床医学
题名:
Expression of an ovarian cancer anti-idiotype antibody (6B11VLVHCH3) in chinese hamster ovary (CHO) cells with improved immunoactivity and stability over proteins expressed in prokaryotic cells
作者: Shi, Jlngjlng; Chang, Xiaohong; Feng, He; Cheng, Yexia; Cheng, Hongyan; Guo, Huifang; Ye, Xue; Cui, Heng
刊名: HYBRIDOMA
发表日期: 2007-10-01
DOI: 10.1089/hyb.2007.0511
卷: 26, 期:5, 页:289-295
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Immunology
研究领域[WOS]: Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Immunology
关键词[WOS]: MONOCLONAL-ANTIBODY ; CARCINOMA ; CRYSTALLIZATION ; GLYCOSYLATION ; DOMAINS
英文摘要:

The 6B11VLVHCH3 is an anti-idiotype antibody to ovarian cancer. It can mimic the ovarian cancer-associated antigen OC166-9. We have previously expressed it in Escherichia coli. We now express the 6B11VLVHCH3 in a Chinese hamster ovary (CHO) cell system. The 6B11VLVHhCH3 sequence was amplified from the prokaryotic vector and cloned into the eukaryotic expression vector pSecTag2. The construct was transfected into CHO cells. Then the transfected monoclonal CHO cells were sequentially adapted to growth in serum-free medium. Functional differences between the antibody produced in E. coli or CHO cells were assessed. The antibody produced by CHO cells was able to both mimic ovarian cancer antigen and retain FC region functionality. Importantly, the antigen mimicry activity of 6B11VLVHhCH3 produced by CHO cells is approximately 20 times higher than antibody expressed by E. coli. This method of expression provides a safer and higher quality antibody for clinical drug development.

语种: 英语
WOS记录号: WOS:000250655200002
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62624
Appears in Collections:北京大学第二临床医学院_妇科肿瘤中心_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: Peking Univ, Peoples Hosp, Gynecol Oncol Ctr, Beijing 100044, Peoples R China

Recommended Citation:
Shi, Jlngjlng,Chang, Xiaohong,Feng, He,et al. Expression of an ovarian cancer anti-idiotype antibody (6B11VLVHCH3) in chinese hamster ovary (CHO) cells with improved immunoactivity and stability over proteins expressed in prokaryotic cells[J]. HYBRIDOMA,2007,26(5):289-295.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Shi, Jlngjlng]'s Articles
[Chang, Xiaohong]'s Articles
[Feng, He]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Shi, Jlngjlng]‘s Articles
[Chang, Xiaohong]‘s Articles
[Feng, He]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace