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学科主题: 临床医学
题名:
Comparative proteome analysis of Helicobacter pylori clinical strains by two-dimensional gel electrophoresis
作者: Zhang, Ya-nan2; Ding, Shi-gang1; Huang, Liu-huan3; Zhang, Jing1; Shi, Yan-yan1; Zhong, Li-jun4
关键词: Helicobacter pylori ; Proteome ; Gastric cancer ; Gastritis ; Two-dimensional gel electrophoresis
刊名: JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B
发表日期: 2011-10-01
DOI: 10.1631/jzus.B1000445
卷: 12, 期:10, 页:820-827
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Medicine, Research & Experimental
研究领域[WOS]: Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Research & Experimental Medicine
关键词[WOS]: TRANSFER-RNA SYNTHESIS ; ESCHERICHIA-COLI ; GENETIC DIVERSITY ; INCREASED RISK ; CAGA ; NUSA ; PROTEINS ; BINDING ; INFECTION ; SEQUENCE
英文摘要:

Objective: To investigate the pathogenic properties of Helicobacter pylori by comparing the proteome map of H. pylori clinical strains. Methods: Two wild-type H. pylori strains, YN8 (isolated from biopsy tissue of a gastric cancer patient) and YN14 (isolated from biopsy tissue of a gastritis and duodenal ulcer patient), were used. Proteomic analysis, using a pH range of 3-10 and 5-8, was performed. The individual proteins were identified by quadrupole time-of-flight (Q-TOF) mass spectrometer and protein database search. Results: Variation in spot patterns directed towards differential protein expression levels was observed between the strains. The gel revealed prominent proteins with several protein "families". The comparison of protein expressions of the two strains reveals a high variability. Differentially present or absent spots were observed. Nine differentially expressed protein spots identified by Q-TOF included adenosine triphosphate (ATP)-binding protein, disulfide oxidoreductase B (DsbB)-like protein, N utilization substance A (NusA), ATP-dependent protease binding subunit/heat shock protein, hydantoin utilization protein A, seryl-tRNA synthetase, molybdenum ABC transporter ModD, and hypothetical proteins. Conclusions: This study suggests that H. pylori strains express/repress protein variation, not only in terms of the virulence proteins, but also in terms of physiological proteins, when they infect a human host. The difference of protein expression levels between H. pylori strains isolated from gastric cancer and gastritis may be the initiator of inflammation, and result in the different clinical presentation. In this preliminary study, we report seven differential proteins between strains, with molecule weights from approximately 10 kDa to approximately 40 kDa. Further studies are needed to investigate those proteins and their function associated with H. pylori colonization and adaptation to host environment stress.

语种: 英语
所属项目编号: 30770980
项目资助者: National Natural Science Foundation of China ; Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry
WOS记录号: WOS:000296251900005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62629
Appears in Collections:北京大学第三临床医学院_消化科_期刊论文

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作者单位: 1.Peking Univ, Dept Gastroenterol, Hosp 3, Beijing 100191, Peoples R China
2.Capital Med Univ, Dept Lab, Beijing Tiantan Hosp, Beijing 100050, Peoples R China
3.Beijing Shi Jing Shan Hosp, Dept Thorac & Cardiovasc Surg, Beijing 100043, Peoples R China
4.Peking Univ, Hlth Sci Ctr, Beijing 100091, Peoples R China

Recommended Citation:
Zhang, Ya-nan,Ding, Shi-gang,Huang, Liu-huan,et al. Comparative proteome analysis of Helicobacter pylori clinical strains by two-dimensional gel electrophoresis[J]. JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B,2011,12(10):820-827.
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