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Comparative proteome analysis of Helicobacter pylori clinical strains by two-dimensional gel electrophoresis
Zhang, Ya-nan2; Ding, Shi-gang1; Huang, Liu-huan3; Zhang, Jing1; Shi, Yan-yan1; Zhong, Li-jun4
关键词Helicobacter pylori Proteome Gastric cancer Gastritis Two-dimensional gel electrophoresis
刊名JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B
2011-10-01
DOI10.1631/jzus.B1000445
12期:10页:820-827
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Medicine, Research & Experimental
资助者National Natural Science Foundation of China ; Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry ; National Natural Science Foundation of China ; Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry
研究领域[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Research & Experimental Medicine
关键词[WOS]TRANSFER-RNA SYNTHESIS ; ESCHERICHIA-COLI ; GENETIC DIVERSITY ; INCREASED RISK ; CAGA ; NUSA ; PROTEINS ; BINDING ; INFECTION ; SEQUENCE
英文摘要

Objective: To investigate the pathogenic properties of Helicobacter pylori by comparing the proteome map of H. pylori clinical strains. Methods: Two wild-type H. pylori strains, YN8 (isolated from biopsy tissue of a gastric cancer patient) and YN14 (isolated from biopsy tissue of a gastritis and duodenal ulcer patient), were used. Proteomic analysis, using a pH range of 3-10 and 5-8, was performed. The individual proteins were identified by quadrupole time-of-flight (Q-TOF) mass spectrometer and protein database search. Results: Variation in spot patterns directed towards differential protein expression levels was observed between the strains. The gel revealed prominent proteins with several protein "families". The comparison of protein expressions of the two strains reveals a high variability. Differentially present or absent spots were observed. Nine differentially expressed protein spots identified by Q-TOF included adenosine triphosphate (ATP)-binding protein, disulfide oxidoreductase B (DsbB)-like protein, N utilization substance A (NusA), ATP-dependent protease binding subunit/heat shock protein, hydantoin utilization protein A, seryl-tRNA synthetase, molybdenum ABC transporter ModD, and hypothetical proteins. Conclusions: This study suggests that H. pylori strains express/repress protein variation, not only in terms of the virulence proteins, but also in terms of physiological proteins, when they infect a human host. The difference of protein expression levels between H. pylori strains isolated from gastric cancer and gastritis may be the initiator of inflammation, and result in the different clinical presentation. In this preliminary study, we report seven differential proteins between strains, with molecule weights from approximately 10 kDa to approximately 40 kDa. Further studies are needed to investigate those proteins and their function associated with H. pylori colonization and adaptation to host environment stress.

语种英语
所属项目编号30770980
资助者National Natural Science Foundation of China ; Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry ; National Natural Science Foundation of China ; Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry
WOS记录号WOS:000296251900005
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62629
专题北京大学第三临床医学院_消化科
作者单位1.Peking Univ, Dept Gastroenterol, Hosp 3, Beijing 100191, Peoples R China
2.Capital Med Univ, Dept Lab, Beijing Tiantan Hosp, Beijing 100050, Peoples R China
3.Beijing Shi Jing Shan Hosp, Dept Thorac & Cardiovasc Surg, Beijing 100043, Peoples R China
4.Peking Univ, Hlth Sci Ctr, Beijing 100091, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Ya-nan,Ding, Shi-gang,Huang, Liu-huan,et al. Comparative proteome analysis of Helicobacter pylori clinical strains by two-dimensional gel electrophoresis[J]. JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B,2011,12(10):820-827.
APA Zhang, Ya-nan,Ding, Shi-gang,Huang, Liu-huan,Zhang, Jing,Shi, Yan-yan,&Zhong, Li-jun.(2011).Comparative proteome analysis of Helicobacter pylori clinical strains by two-dimensional gel electrophoresis.JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B,12(10),820-827.
MLA Zhang, Ya-nan,et al."Comparative proteome analysis of Helicobacter pylori clinical strains by two-dimensional gel electrophoresis".JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B 12.10(2011):820-827.
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