IR@PKUHSC  > 北京大学临床肿瘤学院
学科主题临床医学
A controversial tumor marker: Is SM22 a proper biomarker for gastric cancer cells?
Li, Na; Zhang, Jun; Liang, Yumei; Shao, Jianmin; Peng, Fuli; Sun, Maomao; Xu, Ningzhi; Li, Xianghong; Wang, Rong; Liu, Siqi; Lu, Youyong
关键词gastric cancer biomarker SM22 proteomics tissue microarray
刊名JOURNAL OF PROTEOME RESEARCH
2007-08-01
DOI10.1021/pr0702363
6期:8页:3304-3312
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]DIFFERENCE GEL-ELECTROPHORESIS ; LASER CAPTURE MICRODISSECTION ; SMOOTH-MUSCLE-CELLS ; HUMAN COLON-CANCER ; PROTEOMIC ANALYSIS ; GENE-EXPRESSION ; MASS-SPECTROMETRY ; CARCINOMA ; PROTEIN ; IDENTIFICATION
英文摘要

SM22, a dominant protein in smooth muscle cells (SMCs), has been widely reported to be abnormally expressed in many solid tumors. However, the expression patterns of SM22 are not consistent in all tumors, not even in the same ones. Whether SM22 should be considered a tumor biomarker is still debated in different laboratories. Herein, we have carried out a systematical investigation to validate SM22 expression in the primary tissues of gastric cancer (GC). Of eight cases, seven samples were found in the elevated expression of SM22 proteins through proteomic analysis. The observation was further verified by the approaches of Western blotting and quantitative RT-PCR. Surprisingly, the results achieved from tissue microarray in 126 GC cases appeared contrary to the proteomic conclusion, in which the highly expressed SM22 was mainly found in smooth muscle layers, blood vessels, and myofibroblasts. This suggested that the increased abundance of SM22 in the cancerous regions was not caused by the presence of the GC cells. Furthermore, the expression of SM22 was measured in different GC cell lines and SMCs with Western blotting and quantitative RT-PCR. The results revealed that SM22 expression in SMCs was dramatically higher than that of the GC cells, which indicates that SM22 is unlikely to be a proper biomarker for GC. Instead, it can be considered a potential indicator for the abnormal developments of smooth muscles, blood vessels, or myofibroblasts triggered by tumorigenesis.

语种英语
WOS记录号WOS:000248683200037
引用统计
被引频次:30[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62638
专题北京大学临床肿瘤学院
作者单位1.Beijing Prote Inst, Beijing 101318, Peoples R China
2.Chinese Acad Sci, Beijing Genom Inst, Beijing 101318, Peoples R China
3.Peking Univ, Sch Oncol, Beijing Inst Canc Res, Beijing 100036, Peoples R China
4.Gen Hosp PLA, Dept Pathol, Beijing 100853, Peoples R China
5.CUNY Mt Sinai Sch Med, Dept Genet & Genom Sci, New York, NY 10029 USA
推荐引用方式
GB/T 7714
Li, Na,Zhang, Jun,Liang, Yumei,et al. A controversial tumor marker: Is SM22 a proper biomarker for gastric cancer cells?[J]. JOURNAL OF PROTEOME RESEARCH,2007,6(8):3304-3312.
APA Li, Na.,Zhang, Jun.,Liang, Yumei.,Shao, Jianmin.,Peng, Fuli.,...&Lu, Youyong.(2007).A controversial tumor marker: Is SM22 a proper biomarker for gastric cancer cells?.JOURNAL OF PROTEOME RESEARCH,6(8),3304-3312.
MLA Li, Na,et al."A controversial tumor marker: Is SM22 a proper biomarker for gastric cancer cells?".JOURNAL OF PROTEOME RESEARCH 6.8(2007):3304-3312.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Li, Na]的文章
[Zhang, Jun]的文章
[Liang, Yumei]的文章
百度学术
百度学术中相似的文章
[Li, Na]的文章
[Zhang, Jun]的文章
[Liang, Yumei]的文章
必应学术
必应学术中相似的文章
[Li, Na]的文章
[Zhang, Jun]的文章
[Liang, Yumei]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。