IR@PKUHSC  > 北京大学基础医学院  > 生物物理学系
学科主题基础医学
A 130-kDa Protein 4.1B Regulates Cell Adhesion, Spreading, and Migration of Mouse Embryo Fibroblasts by Influencing Actin Cytoskeleton Organization
Wang, Jie1,2; Song, Jinlei1; An, Chao3; Dong, Wenji4,5; Zhang, Jingxin1; Yin, Changcheng1; Hale, John3; Baines, Anthony J.6; Mohandas, Narla3; An, Xiuli2,7
关键词Actin Adhesion Cytoskeleton Membrane Migration
刊名JOURNAL OF BIOLOGICAL CHEMISTRY
2014-02-28
DOI10.1074/jbc.M113.516617
289期:9页:5925-5937
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]C-TERMINAL-DOMAIN ; TUMOR-SUPPRESSOR ; FUNCTIONAL-CHARACTERIZATION ; HEREDITARY ELLIPTOCYTOSIS ; MEMBRANE-PROTEINS ; MYELINATED AXONS ; LUNG-CANCER ; FERM DOMAIN ; BAND 4.1B ; FAMILY
英文摘要

Background: The function of protein 4.1B in behaviors of motile cells remains poorly understood. Results: Deficiency of 130-kDa 4.1B resulted in impaired cell adhesion, spreading, migration, and stress fiber formation. Conclusion: Protein 4.1B plays an important role in modulating cell adhesion and motility. Significance: Our results identify a novel function for protein 4.1B and have implications for understanding the mechanisms of 4.1 function.

Protein 4.1B is a member of protein 4.1 family, adaptor proteins at the interface of membranes and the cytoskeleton. It is expressed in most mammalian tissues and is known to be required in formation of nervous and cardiac systems; it is also a tumor suppressor with a role in metastasis. Here, we explore functions of 4.1B using primary mouse embryonic fibroblasts (MEF) derived from wild type and 4.1B knock-out mice. MEF cells express two 4.1B isoforms: 130 and 60-kDa. 130-kDa 4.1B was absent from 4.1B knock-out MEF cells, but 60-kDa 4.1B remained, suggesting incomplete knock-out. Although the 130-kDa isoform was predominantly located at the plasma membrane, the 60-kDa isoform was enriched in nuclei. 130-kDa-deficient 4.1B MEF cells exhibited impaired cell adhesion, spreading, and migration; they also failed to form actin stress fibers. Impaired cell spreading and stress fiber formation were rescued by re-expression of the 130-kDa 4.1B but not the 60-kDa 4.1B. Our findings document novel, isoform-selective roles for 130-kDa 4.1B in adhesion, spreading, and migration of MEF cells by affecting actin organization, giving new insight into 4.1B functions in normal tissues as well as its role in cancer.

语种英语
WOS记录号WOS:000332015500049
项目编号DK 26263 ; DK 32094 ; 81171905 ; 81272187
资助机构National Institutes of Health ; Natural Science Foundation of China ; American Society of Hematology (ASH)
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62642
专题北京大学基础医学院_生物物理学系
作者单位1.Peking Univ, Hlth Sci Ctr, Dept Biophys, Beijing 100191, Peoples R China
2.New York Blood Ctr, Membrane Biol Lab, New York, NY 10065 USA
3.New York Blood Ctr, Red Cell Physiol Lab, New York, NY 10065 USA
4.Chinese Acad Med Sci, Inst Basic Med Sci, Dept Cell Biol, Beijing 100005, Peoples R China
5.Peking Union Med Coll, Beijing 100005, Peoples R China
6.Univ Kent, Sch Biosci, Canterbury CT2 7NJ, Kent, England
7.Zhengzhou Univ, Coll Life Sci, Zhengzhou 450051, Peoples R China
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GB/T 7714
Wang, Jie,Song, Jinlei,An, Chao,et al. A 130-kDa Protein 4.1B Regulates Cell Adhesion, Spreading, and Migration of Mouse Embryo Fibroblasts by Influencing Actin Cytoskeleton Organization[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2014,289(9):5925-5937.
APA Wang, Jie.,Song, Jinlei.,An, Chao.,Dong, Wenji.,Zhang, Jingxin.,...&An, Xiuli.(2014).A 130-kDa Protein 4.1B Regulates Cell Adhesion, Spreading, and Migration of Mouse Embryo Fibroblasts by Influencing Actin Cytoskeleton Organization.JOURNAL OF BIOLOGICAL CHEMISTRY,289(9),5925-5937.
MLA Wang, Jie,et al."A 130-kDa Protein 4.1B Regulates Cell Adhesion, Spreading, and Migration of Mouse Embryo Fibroblasts by Influencing Actin Cytoskeleton Organization".JOURNAL OF BIOLOGICAL CHEMISTRY 289.9(2014):5925-5937.
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