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学科主题: 基础医学
题名:
A 130-kDa Protein 4.1B Regulates Cell Adhesion, Spreading, and Migration of Mouse Embryo Fibroblasts by Influencing Actin Cytoskeleton Organization
作者: Wang, Jie1,2; Song, Jinlei1; An, Chao3; Dong, Wenji4,5; Zhang, Jingxin1; Yin, Changcheng1; Hale, John3; Baines, Anthony J.6; Mohandas, Narla3; An, Xiuli2,7
关键词: Actin ; Adhesion ; Cytoskeleton ; Membrane ; Migration
刊名: JOURNAL OF BIOLOGICAL CHEMISTRY
发表日期: 2014-02-28
DOI: 10.1074/jbc.M113.516617
卷: 289, 期:9, 页:5925-5937
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: C-TERMINAL-DOMAIN ; TUMOR-SUPPRESSOR ; FUNCTIONAL-CHARACTERIZATION ; HEREDITARY ELLIPTOCYTOSIS ; MEMBRANE-PROTEINS ; MYELINATED AXONS ; LUNG-CANCER ; FERM DOMAIN ; BAND 4.1B ; FAMILY
英文摘要:

Background: The function of protein 4.1B in behaviors of motile cells remains poorly understood. Results: Deficiency of 130-kDa 4.1B resulted in impaired cell adhesion, spreading, migration, and stress fiber formation. Conclusion: Protein 4.1B plays an important role in modulating cell adhesion and motility. Significance: Our results identify a novel function for protein 4.1B and have implications for understanding the mechanisms of 4.1 function.

Protein 4.1B is a member of protein 4.1 family, adaptor proteins at the interface of membranes and the cytoskeleton. It is expressed in most mammalian tissues and is known to be required in formation of nervous and cardiac systems; it is also a tumor suppressor with a role in metastasis. Here, we explore functions of 4.1B using primary mouse embryonic fibroblasts (MEF) derived from wild type and 4.1B knock-out mice. MEF cells express two 4.1B isoforms: 130 and 60-kDa. 130-kDa 4.1B was absent from 4.1B knock-out MEF cells, but 60-kDa 4.1B remained, suggesting incomplete knock-out. Although the 130-kDa isoform was predominantly located at the plasma membrane, the 60-kDa isoform was enriched in nuclei. 130-kDa-deficient 4.1B MEF cells exhibited impaired cell adhesion, spreading, and migration; they also failed to form actin stress fibers. Impaired cell spreading and stress fiber formation were rescued by re-expression of the 130-kDa 4.1B but not the 60-kDa 4.1B. Our findings document novel, isoform-selective roles for 130-kDa 4.1B in adhesion, spreading, and migration of MEF cells by affecting actin organization, giving new insight into 4.1B functions in normal tissues as well as its role in cancer.

语种: 英语
所属项目编号: DK 26263 ; DK 32094 ; 81171905 ; 81272187
项目资助者: National Institutes of Health ; Natural Science Foundation of China ; American Society of Hematology (ASH)
WOS记录号: WOS:000332015500049
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62642
Appears in Collections:基础医学院_生物物理学系_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Dept Biophys, Beijing 100191, Peoples R China
2.New York Blood Ctr, Membrane Biol Lab, New York, NY 10065 USA
3.New York Blood Ctr, Red Cell Physiol Lab, New York, NY 10065 USA
4.Chinese Acad Med Sci, Inst Basic Med Sci, Dept Cell Biol, Beijing 100005, Peoples R China
5.Peking Union Med Coll, Beijing 100005, Peoples R China
6.Univ Kent, Sch Biosci, Canterbury CT2 7NJ, Kent, England
7.Zhengzhou Univ, Coll Life Sci, Zhengzhou 450051, Peoples R China

Recommended Citation:
Wang, Jie,Song, Jinlei,An, Chao,et al. A 130-kDa Protein 4.1B Regulates Cell Adhesion, Spreading, and Migration of Mouse Embryo Fibroblasts by Influencing Actin Cytoskeleton Organization[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2014,289(9):5925-5937.
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