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学科主题: 基础医学
题名:
H5N1 infection of the respiratory tract and beyond: a molecular pathology study
作者: Gu, Jiang; Xie, Zhigang; Gao, Zhancheng; Liu, Jinhua; Korteweg, Christine; Ye, Juxiang; Lau, Lok Ting; Lu, Jie; Gao, Zifen; Zhang, Bo; McNutt, Michael A.; Lu, Min; Anderson, Virginia M.; Gong, Encong; Yu, Albert Cheung Hoi; Lipkin, W. Ian
刊名: LANCET
发表日期: 2007-09-29
卷: 370, 期:9593, 页:1137-1145
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: INFLUENZA-A H5N1 ; HUMAN AIRWAY EPITHELIUM ; AVIAN INFLUENZA ; HUMAN-DISEASE ; VIRUS INFECTION ; IN-VITRO ; TRANSMISSION ; EXPRESSION ; SARS ; PATHOGENESIS
英文摘要:

Background Human infection with avian influenza H5N1 is an emerging infectious disease characterised by respiratory symptoms and a high fatality rate. Previous studies have shown that the human infection with avian influenza H5N1 could also target organs apart from the lungs.

Methods We studied post-mortem tissues of two adults (one man and one pregnant woman) infected with H5N1 influenza virus, and a fetus carried by the woman. In-situ hybridisation (with sense and antisense probes to haemagglutinin and nucleoprotein) and immunohistochemistry (with monoclonal antibodies to haemagglutinin and nucleoprotein) were done on selected tissues. Reverse-transcriptase (RT) PCR, real-time RT PCR, strand-specific RT PCR, and nucleic acid sequence-based amplification (NASBA) detection assays were also undertaken to detect viral RNA in organ tissue samples.

Findings We detected viral genomic sequences and antigens in type II epithelial cells of the lungs, ciliated and non-ciliated epithelial cells of the trachea, T cells of the lymph node, neurons of the brain, and Hofbauer cells and cytotrophoblasts of the placenta. Viral genomic sequences (but no viral antigens) were detected in the intestinal mucosa. In the fetus, we found viral sequences and antigens in the lungs, circulating mononuclear cells, and macrophages of the liver. The presence of viral sequences in the organs and the fetus was also confirmed by RT PCR, strand-specific RT PCR, real-time RT PCR, and NASBA.

Interpretation In addition to the lungs, H5N1 influenza virus infects the trachea and disseminates to other organs including the brain. The virus could also be transmitted from mother to fetus across the placenta.

语种: 英语
WOS记录号: WOS:000250263700028
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62650
Appears in Collections:基础医学院_北京大学感染病研究中心_期刊论文

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作者单位: 1.Peking Univ, Ctr Infect Dis, Beijing 100083, Peoples R China
2.Peking Univ, Peoples Hosp, Beijing 100083, Peoples R China
3.Peking Univ, Neurosci Res Inst, Beijing 100083, Peoples R China
4.China Agr Univ, Coll Vet Med, Beijing, Peoples R China
5.Columbia Univ, Mailman Sch Publ Hlth, New York, NY USA
6.Peking Univ, Sch Basic Med Sci, Dept Pathol, Beijing 100083, Peoples R China
7.SUNY, Hlth Sci Ctr, Dept Pathol, New York, NY USA

Recommended Citation:
Gu, Jiang,Xie, Zhigang,Gao, Zhancheng,et al. H5N1 infection of the respiratory tract and beyond: a molecular pathology study[J]. LANCET,2007,370(9593):1137-1145.
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