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Rosiglitazone Attenuates Endothelin-1-Induced Vasoconstriction by Upregulating Endothelial Expression of Endothelin B Receptor
Tian, Jianwei1,2,3; Wong, Wing Tak4,5; Tian, Xiao Yu4,5; Zhang, Peng1,2; Huang, Yu4,5; Wang, Nanping1,2
关键词thiazolidinediones vasoconstriction ET(B)R PPAR-gamma
刊名HYPERTENSION
2010-07-01
DOI10.1161/HYPERTENSIONAHA.110.150375
56期:1页:129-U205
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Peripheral Vascular Disease
资助者National Natural Science Foundation of China/Research Grants Council Joint Research Scheme ; National Natural Science Foundation of China ; National Natural Science Foundation of China/Research Grants Council Joint Research Scheme ; National Natural Science Foundation of China
研究领域[WOS]Cardiovascular System & Cardiology
关键词[WOS]TYPE-2 DIABETES-MELLITUS ; LOWERS BLOOD-PRESSURE ; PPAR-GAMMA ; SIGNALING PATHWAY ; HYPERTENSIVE-RATS ; FATTY RATS ; VASCULATURE ; CELLS ; DYSFUNCTION ; RELAXATION
英文摘要

Thiazolidinediones improve insulin resistance and endothelial dysfunction. However, the mechanisms underlying the vasoprotective effects of thiazolidinediones remain to be fully elucidated. The present study aimed to examine the molecular mechanism for the anti-vasoconstrictive effects of rosiglitazone in response to endothelin (ET) 1. Mouse aortas were treated with rosiglitazone for 24 hours, and ET-1-induced vasoconstriction was assessed by wire myography. The results showed that rosiglitazone attenuated ET-1-induced contraction in mouse aortas; this effect was abolished by ET-B receptor (ET(B)R) antagonist, NO synthase inhibitor, and by the removal of endothelium. By using Northern blotting, real-time RT-PCR, Western blotting, and immunohistochemical techniques, we found that rosiglitazone upregulated expression of ET(B)R at both mRNA and protein levels in mouse aortas and human vascular endothelial cells. The induction of ET(B)R was prevented by peroxisome proliferator-activated receptor-gamma antagonism. Luciferase reporter assay showed that rosiglitazone enhanced ETBR gene promoter activity. Furthermore, chromatin immunoprecipitation assays demonstrated that peroxisome proliferator-activated receptor-gamma can directly bind to ET(B)R gene promoter. Furthermore, in vivo treatment with rosiglitazone also attenuated the ET-1-induced vasoconstrictions and increased the ET(B)R expression in mouse aortas and mesenteric arteries. In conclusion, these results demonstrate that rosiglitazone attenuated ET-1-induced vasoconstriction through the upregulation of endothelial ET(B)R, which is a peroxisome proliferator-activated receptor-gamma direct target. (Hypertension. 2010; 56: 129-135.)

语种英语
所属项目编号30931160434 ; N_CUHK428/09 ; 30890041 ; 30821001
资助者National Natural Science Foundation of China/Research Grants Council Joint Research Scheme ; National Natural Science Foundation of China ; National Natural Science Foundation of China/Research Grants Council Joint Research Scheme ; National Natural Science Foundation of China
WOS记录号WOS:000278859300023
引用统计
被引频次:16[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62655
专题基础医学院_心血管所
作者单位1.Peking Univ, Minist Educ, Inst Cardiovasc Sci, Beijing 100191, Peoples R China
2.Peking Univ, Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing 100191, Peoples R China
3.AF Gen Hosp, Div Cardiol, Beijing, Peoples R China
4.Chinese Univ Hong Kong, Inst Vasc Med, Shatin, Hong Kong, Peoples R China
5.Chinese Univ Hong Kong, Sch Biomed Sci, Shatin, Hong Kong, Peoples R China
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GB/T 7714
Tian, Jianwei,Wong, Wing Tak,Tian, Xiao Yu,et al. Rosiglitazone Attenuates Endothelin-1-Induced Vasoconstriction by Upregulating Endothelial Expression of Endothelin B Receptor[J]. HYPERTENSION,2010,56(1):129-U205.
APA Tian, Jianwei,Wong, Wing Tak,Tian, Xiao Yu,Zhang, Peng,Huang, Yu,&Wang, Nanping.(2010).Rosiglitazone Attenuates Endothelin-1-Induced Vasoconstriction by Upregulating Endothelial Expression of Endothelin B Receptor.HYPERTENSION,56(1),129-U205.
MLA Tian, Jianwei,et al."Rosiglitazone Attenuates Endothelin-1-Induced Vasoconstriction by Upregulating Endothelial Expression of Endothelin B Receptor".HYPERTENSION 56.1(2010):129-U205.
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