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学科主题基础医学
HDTIC-1 and HDTIC-2, two compounds extracted from Astragali Radix, delay replicative senescence of human diploid fibroblasts
Wang, PC; Zhang, ZY; Ma, XF; Yu, H; Liu, XW; Tu, PF; Tong, TJ
关键词HDTIC astragali radix senescence fibroblast carnosine
刊名MECHANISMS OF AGEING AND DEVELOPMENT
2003-12-01
DOI10.1016/j.mad.2003.08.005
124期:10-12页:1025-1034
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology ; Geriatrics & Gerontology
研究领域[WOS]Cell Biology ; Geriatrics & Gerontology
关键词[WOS]PROTEIN OXIDATION ; DIABETES-MELLITUS ; AGE-RECEPTORS ; DNA-SYNTHESIS ; GROWTH-FACTOR ; CELLS ; EXPRESSION ; GLYCATION ; DAMAGE ; VIABILITY
英文摘要

Astragalus membranceus (Fish) Bunge Var. mongholicus (Bge) Hsiao is a Chinese herb considered as an effective traditional anti-ageing material. The two isomers of 4-hydroxy-5-hydroxymethyl-[1,3]dioxolan-2,6′-spirane-5′,6′,7′,8′-tetrahydro-indolizine-3′-carbaldehyde (HDTIC), HDTIC-1 and HDTIC-2, were extracted from the herb. We chose them to investigate their effects on replicative senescence in vitro. In this study, we observed the effects of HDTIC-1 and HDTIC-2 on morphology, replicative lifespan, and specific markers related to replicative senescence in human fetal lung diploid fibroblast (2BS cell). Results have shown that both the HDTIC-1. and HDTIC-2 maintain non-senescent phenotype of 2BS cells even at late population doubling (PD) and increase cumulative population doublings (CPDs) by at least 15-20 PDs. The senescence-associated-galactosidase (SA-beta-gal) positive cell rates of late PD cells grown from early PD in medium containing HDTIC. were much lower than that of late PD control cells, and similar to that of young cells. HDTIC a,so improved cell growth and proliferation and promoted the entry of 2BS cells from Go or G, phase to S-phase. In addition, the advanced glycation end product (AGE) levels of late PD cells grown from early PD in DMEM containing HDTIC decreased significantly compared with those of late PD control cells. Taken together, the results strongly suggest that both the HDTIC-1 and HDTIC-2 delay replicative senescence of 2BS cells, and indicate that the senescence-delaying effect of HDTIC appears to be due to its many biological properties including its potentials of proliferation improvement, inhibitory effect of AGE formation, and its antioxidant activity. The differences of optimum concentrations of HDTIC-1 (0.1 muM) and HDTIC-2 (1.0 muM) for delaying senescence also indicate that the structure of HDTIC may be very sensitive to its activity. (C) 2003 Elsevier Ireland Ltd. All rights reserved.

语种英语
WOS记录号WOS:000187587400005
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被引频次:29[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
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条目标识符http://ir.bjmu.edu.cn/handle/400002259/62659
专题北京大学基础医学院_中西医结合学系
作者单位1.Peking Univ, Hlth Sci Ctr, Dept Biochem & Mol Biol, Beijing 100083, Peoples R China
2.Peking Univ, Modern Res Ctr Tradit Chinese Med, Beijing 100083, Peoples R China
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Wang, PC,Zhang, ZY,Ma, XF,et al. HDTIC-1 and HDTIC-2, two compounds extracted from Astragali Radix, delay replicative senescence of human diploid fibroblasts[J]. MECHANISMS OF AGEING AND DEVELOPMENT,2003,124(10-12):1025-1034.
APA Wang, PC.,Zhang, ZY.,Ma, XF.,Yu, H.,Liu, XW.,...&Tong, TJ.(2003).HDTIC-1 and HDTIC-2, two compounds extracted from Astragali Radix, delay replicative senescence of human diploid fibroblasts.MECHANISMS OF AGEING AND DEVELOPMENT,124(10-12),1025-1034.
MLA Wang, PC,et al."HDTIC-1 and HDTIC-2, two compounds extracted from Astragali Radix, delay replicative senescence of human diploid fibroblasts".MECHANISMS OF AGEING AND DEVELOPMENT 124.10-12(2003):1025-1034.
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