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学科主题: 基础医学
题名:
HDTIC-1 and HDTIC-2, two compounds extracted from Astragali Radix, delay replicative senescence of human diploid fibroblasts
作者: Wang, PC; Zhang, ZY; Ma, XF; Yu, H; Liu, XW; Tu, PF; Tong, TJ
关键词: HDTIC ; astragali radix ; senescence ; fibroblast ; carnosine
刊名: MECHANISMS OF AGEING AND DEVELOPMENT
发表日期: 2003-12-01
DOI: 10.1016/j.mad.2003.08.005
卷: 124, 期:10-12, 页:1025-1034
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology ; Geriatrics & Gerontology
研究领域[WOS]: Cell Biology ; Geriatrics & Gerontology
关键词[WOS]: PROTEIN OXIDATION ; DIABETES-MELLITUS ; AGE-RECEPTORS ; DNA-SYNTHESIS ; GROWTH-FACTOR ; CELLS ; EXPRESSION ; GLYCATION ; DAMAGE ; VIABILITY
英文摘要:

Astragalus membranceus (Fish) Bunge Var. mongholicus (Bge) Hsiao is a Chinese herb considered as an effective traditional anti-ageing material. The two isomers of 4-hydroxy-5-hydroxymethyl-[1,3]dioxolan-2,6′-spirane-5′,6′,7′,8′-tetrahydro-indolizine-3′-carbaldehyde (HDTIC), HDTIC-1 and HDTIC-2, were extracted from the herb. We chose them to investigate their effects on replicative senescence in vitro. In this study, we observed the effects of HDTIC-1 and HDTIC-2 on morphology, replicative lifespan, and specific markers related to replicative senescence in human fetal lung diploid fibroblast (2BS cell). Results have shown that both the HDTIC-1. and HDTIC-2 maintain non-senescent phenotype of 2BS cells even at late population doubling (PD) and increase cumulative population doublings (CPDs) by at least 15-20 PDs. The senescence-associated-galactosidase (SA-beta-gal) positive cell rates of late PD cells grown from early PD in medium containing HDTIC. were much lower than that of late PD control cells, and similar to that of young cells. HDTIC a,so improved cell growth and proliferation and promoted the entry of 2BS cells from Go or G, phase to S-phase. In addition, the advanced glycation end product (AGE) levels of late PD cells grown from early PD in DMEM containing HDTIC decreased significantly compared with those of late PD control cells. Taken together, the results strongly suggest that both the HDTIC-1 and HDTIC-2 delay replicative senescence of 2BS cells, and indicate that the senescence-delaying effect of HDTIC appears to be due to its many biological properties including its potentials of proliferation improvement, inhibitory effect of AGE formation, and its antioxidant activity. The differences of optimum concentrations of HDTIC-1 (0.1 muM) and HDTIC-2 (1.0 muM) for delaying senescence also indicate that the structure of HDTIC may be very sensitive to its activity. (C) 2003 Elsevier Ireland Ltd. All rights reserved.

语种: 英语
WOS记录号: WOS:000187587400005
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62659
Appears in Collections:基础医学院_中西医结合学系_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Dept Biochem & Mol Biol, Beijing 100083, Peoples R China
2.Peking Univ, Modern Res Ctr Tradit Chinese Med, Beijing 100083, Peoples R China

Recommended Citation:
Wang, PC,Zhang, ZY,Ma, XF,et al. HDTIC-1 and HDTIC-2, two compounds extracted from Astragali Radix, delay replicative senescence of human diploid fibroblasts[J]. MECHANISMS OF AGEING AND DEVELOPMENT,2003,124(10-12):1025-1034.
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