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学科主题: 基础医学
题名:
SPC-P1: a pathogenicity-associated prophage of Salmonella paratyphi C
作者: Zou, Qing-Hua2; Li, Qing-Hai1,6; Zhu, Hong-Yun2; Feng, Ye1,6; Li, Yong-Guo3; Johnston, Randal N.4; Liu, Gui-Rong1,6; Liu, Shu-Lin1,2,3,5,6
刊名: BMC GENOMICS
发表日期: 2010-12-30
DOI: 10.1186/1471-2164-11-729
卷: 11, 期:1
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biotechnology & Applied Microbiology ; Genetics & Heredity
研究领域[WOS]: Biotechnology & Applied Microbiology ; Genetics & Heredity
关键词[WOS]: COMPLETE GENOME SEQUENCE ; DETERMINING DIVERGENCE TIMES ; SEROVAR TYPHIMURIUM DT-64 ; MOLECULAR CHARACTERIZATION ; PROTEIN CLOCK ; ENTERICA ; TYPHI
英文摘要:

Background: Salmonella paratyphi C is one of the few human-adapted pathogens along with S. typhi, S. paratyphi A and S. paratyphi B that cause typhoid, but it is not clear whether these bacteria cause the disease by the same or different pathogenic mechanisms. Notably, these typhoid agents have distinct sets of large genomic insertions, which may encode different pathogenicity factors. Previously we identified a novel prophage, SPC-P1, in S. paratyphi C RKS4594 and wondered whether it might be involved in pathogenicity of the bacteria.

Results: We analyzed the sequence of SPC-P1 and found that it is an inducible phage with an overall G+C content of 47.24%, similar to that of most Salmonella phages such as P22 and ST64T but significantly lower than the 52.16% average of the RKS4594 chromosome. Electron microscopy showed short-tailed phage particles very similar to the lambdoid phage CUS-3. To evaluate its roles in pathogenicity, we lysogenized S. paratyphi C strain CN13/87, which did not have this prophage, and infected mice with the lysogenized CN13/87. Compared to the phage-free wild type CN13/87, the lysogenized CN13/87 exhibited significantly increased virulence and caused multi-organ damages in mice at considerably lower infection doses.

Conclusions: SPC-P1 contributes pathogenicity to S. paratyphi C in animal infection models, so it is possible that this prophage is involved in typhoid pathogenesis in humans. Genetic and functional analyses of SPC-P1 may facilitate the study of pathogenic evolution of the extant typhoid agents, providing particular help in elucidating the pathogenic determinants of the typhoid agents.

语种: 英语
所属项目编号: NSFC30970078 ; NSFC30870098 ; 30970119 ; 81030029 ; 20092307110001
项目资助者: Canadian Institutes of Health Research ; National Natural Science Foundation of China ; Natural Science Foundation of Heilongjiang Province of China ; Harbin Medical University ; Specialized Research Fund for the Doctoral Program of Higher Education (SRFDP) ; Peking University Health Science Center
WOS记录号: WOS:000286322500002
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62674
Appears in Collections:基础医学院_病原生物学系_期刊论文

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作者单位: 1.Univ Calgary, Dept Biochem & Mol Biol, Calgary, AB, Canada
2.Univ Calgary, Dept Microbiol & Infect Dis, Calgary, AB, Canada
3.Harbin Med Univ, Genom Res Ctr, Harbin, Peoples R China
4.Peking Univ, Hlth Sci Ctr, Dept Microbiol, Beijing 100871, Peoples R China
5.Harbin Med Univ, Genet Detect Ctr, Hosp 1, Harbin, Peoples R China
6.Harbin Med Univ, Syst Res Ctr, Sch Pharm, Harbin, Peoples R China

Recommended Citation:
Zou, Qing-Hua,Li, Qing-Hai,Zhu, Hong-Yun,et al. SPC-P1: a pathogenicity-associated prophage of Salmonella paratyphi C[J]. BMC GENOMICS,2010,11(1).
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