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Determination of binding constant and binding region of programmed cell death 5-heparin by capillary zone electrophoresis
Liu Yi; Ling Xiaomei; Fan Hui; Zhong Yingcheng; Li Dan; Wang Ying
关键词capillary zone electrophoresis PDCD5 heparin
刊名JOURNAL OF CHROMATOGRAPHY A
2007-03-02
DOI10.1016/j.chroma.2007.01.005
1143期:1-2页:284-287
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]GROWTH-FACTOR ; INTERNALIZATION ; PROTEIN
英文摘要

A sensitive and selective high-performance analytical method based on capillary zone electrophoresis (CZE) was developed for investigating interactions between heparin and programmed cell death 5 (PDCD5) qualitatively and quantitatively. The binding constant of the interaction between PDCD5 and heparin calculated by Scatchard analysis was 4.17 x 10(4) M-1 and the binding sites located in the C-terminal region of PDCD5 (residues 109-115). The precisions of migration times, peak heights and binding constants, expressed as the relative standard deviation, were less than 2.4%, 1.1% and 5.7%, respectively. (c) 2007 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000244546700035
Citation statistics
Cited Times:13[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62688
Collection北京大学药学院_化学生物学系
作者单位1.Peking Univ, Sch Pharmaceut Sci, Dept Pharmaceut Anal, Beijing 100083, Peoples R China
2.Peking Univ, Ctr Human Dis Genom, Beijing 100083, Peoples R China
Recommended Citation
GB/T 7714
Liu Yi,Ling Xiaomei,Fan Hui,et al. Determination of binding constant and binding region of programmed cell death 5-heparin by capillary zone electrophoresis[J]. JOURNAL OF CHROMATOGRAPHY A,2007,1143(1-2):284-287.
APA Liu Yi,Ling Xiaomei,Fan Hui,Zhong Yingcheng,Li Dan,&Wang Ying.(2007).Determination of binding constant and binding region of programmed cell death 5-heparin by capillary zone electrophoresis.JOURNAL OF CHROMATOGRAPHY A,1143(1-2),284-287.
MLA Liu Yi,et al."Determination of binding constant and binding region of programmed cell death 5-heparin by capillary zone electrophoresis".JOURNAL OF CHROMATOGRAPHY A 1143.1-2(2007):284-287.
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