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Phenotypic and functional analysis of human T lymphocytes in early second- and third-trimester fetuses
Zhao, Y; Dai, ZP; Lv, P; Gao, XM
关键词fetal T lymphocytes phenotype cytokine proliferation
刊名CLINICAL AND EXPERIMENTAL IMMUNOLOGY
2002-08-01
129期:2页:302-308
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Immunology
研究领域[WOS]Immunology
关键词[WOS]MONONUCLEAR CELL SUBPOPULATIONS ; FETAL PERIPHERAL-BLOOD ; CORD-BLOOD ; PROLIFERATIVE RESPONSES ; PREGNANCY ; SUBSETS ; ADULTS ; INTERLEUKIN-6 ; EXPRESSION ; NEWBORNS
英文摘要

This study was undertaken to investigate the phenotypic and functional status of T lymphocytes of human fetuses from early second- to third-trimester. Cord blood samples were obtained from 19 healthy human fetuses (gestation weeks: 18-36), by cordocentesis, and 16 term newborns (gestation weeks 37-42). Maternal and unrelated male blood samples were also taken as controls. Percentage of lymphocytes in fetal white blood cells was 79.3%, reducing to 40% by term birth, much higher than that of adults. Cord blood mononuclear cells (CBMC), prepared by density gradient centrifugation followed by lysis of erythrocytes, were stained using PE- or FITC-labelled monoclonal Abs and analysed by flow cytometry. The frequencies of CD3(+) T cells in fetal (40.1%) and neonatal (42.4%) CBMC were significantly lower than that of men (59.6%) and pregnant women (53.6%). Proportions of CD8(+) T cells (9.5%), gammadelta-T cells (0.5%) and NK cells (4.8%) in fetal CBMC were also lower than that of neonates (except gammadelta-T cells) and adults. A negative linear correlation (r = -0.609) between the ratio of CD4(+)/CD8(+) T cells in fetal blood and gestation age could also be established. Fetal CBMC showed vigorous spontaneous proliferation but failed to respond to mitogen (PHA) or allogeneic stimulation in vitro. The fetal mononuclear cells were unable to produce IL-2, IL-4 or IFN-gamma, but spontaneously secreted IL-10, IL-6 and TNF-alpha in vitro. Stimulation with PHA up-regulated the production of IL-10, TL-6 and TNF-alpha substantially.

语种英语
WOS记录号WOS:000177647400015
引用统计
被引频次:28[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62698
专题基础医学院_免疫学系
作者单位1.Peking Univ, Hlth Sci Ctr, Dept Immunol, Beijing 100083, Peoples R China
2.Peking Univ, Peoples Hosp, Dept Obstet & Gynaecol, Beijing 100871, Peoples R China
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GB/T 7714
Zhao, Y,Dai, ZP,Lv, P,et al. Phenotypic and functional analysis of human T lymphocytes in early second- and third-trimester fetuses[J]. CLINICAL AND EXPERIMENTAL IMMUNOLOGY,2002,129(2):302-308.
APA Zhao, Y,Dai, ZP,Lv, P,&Gao, XM.(2002).Phenotypic and functional analysis of human T lymphocytes in early second- and third-trimester fetuses.CLINICAL AND EXPERIMENTAL IMMUNOLOGY,129(2),302-308.
MLA Zhao, Y,et al."Phenotypic and functional analysis of human T lymphocytes in early second- and third-trimester fetuses".CLINICAL AND EXPERIMENTAL IMMUNOLOGY 129.2(2002):302-308.
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