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学科主题基础医学
Comparison Analysis of Dysregulated LncRNA Profile in Mouse Plasma and Liver after Hepatic Ischemia/Reperfusion Injury
Chen, Zhenzhen1,4; Luo, Yanjin1,4; Yang, Weili1,4; Ding, Liwei1,4; Wang, Junpei1,2; Tu, Jian1,2; Geng, Bin1,4,5; Cui, Qinghua1,2,3,4,5; Yang, Jichun1,4,5
刊名PLOS ONE
2015-07-29
DOI10.1371/journal.pone.0133462
10期:7
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]LONG NONCODING RNAS ; EXPRESSION ; ISCHEMIA ; CANCER ; STROKE ; HEART
英文摘要

Long noncoding RNAs (LncRNAs) have been believed to be the major transcripts in various tissues and organs, and may play important roles in regulation of many biological processes. The current study determined the LncRNA profile in mouse plasma after liver ischemia/reperfusion injury (IRI) using microarray technology. Microarray assays revealed that 64 LncRNAs were upregulated, and 244 LncRNAs were downregulated in the plasma of liver IRI mouse. Among these dysregulated plasma LncRNAs, 59-61% were intergenic, 22-25% were antisense overlap, 8-12% were sense overlap and 6-7% were bidirectional. Ten dysregulated plasma LncRNAs were validated by quantitative PCR assays, confirming the accuracy of microarray analysis result. Comparison analysis between dysregulated plasma and liver LncRNA profile after liver IRI revealed that among the 308 dysregulated plasma LncRNAs, 245 LncRNAs were present in the liver, but remained unchanged. In contrast, among the 98 dysregulated liver LncRNAs after IRI, only 19 were present in the plasma, but remained unchanged. LncRNA AK139328 had been previously reported to be upregulated in the liver after IRI, and silencing of hepatic AK139328 ameliorated liver IRI. Both microarray and RTPCR analyses failed to detect the presence of AK139328 in mouse plasma. In summary, the current study compared the difference between dysregulated LncRNA profile in mouse plasma and liver after liver IRI, and suggested that a group of dysregulated plasma LncRNAs have the potential of becoming novel biomarkers for evaluation of ischemic liver injury.

语种英语
WOS记录号WOS:000358836800051
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62701
专题北京大学基础医学院
作者单位1.Peking Univ, Dept Physiol & Pathophysiol, Sch Basic Med Sci, Beijing 100191, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Dept Biomed Informat, Beijing 100191, Peoples R China
3.Peking Univ, Inst Syst Biomed, Beijing 100191, Peoples R China
4.Peking Univ, MOE Key Lab Mol Cardiovasc Sci, Beijing 100191, Peoples R China
5.Peking Univ, Ctr Noncoding RNA Med, Hlth Sci Ctr, Beijing 100191, Peoples R China
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Chen, Zhenzhen,Luo, Yanjin,Yang, Weili,et al. Comparison Analysis of Dysregulated LncRNA Profile in Mouse Plasma and Liver after Hepatic Ischemia/Reperfusion Injury[J]. PLOS ONE,2015,10(7).
APA Chen, Zhenzhen.,Luo, Yanjin.,Yang, Weili.,Ding, Liwei.,Wang, Junpei.,...&Yang, Jichun.(2015).Comparison Analysis of Dysregulated LncRNA Profile in Mouse Plasma and Liver after Hepatic Ischemia/Reperfusion Injury.PLOS ONE,10(7).
MLA Chen, Zhenzhen,et al."Comparison Analysis of Dysregulated LncRNA Profile in Mouse Plasma and Liver after Hepatic Ischemia/Reperfusion Injury".PLOS ONE 10.7(2015).
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