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IR@PKUHSC  > 北京大学口腔医学院  > 牙周科  > 期刊论文
学科主题: 口腔医学
题名:
Porphyromonas gingivalis Exacerbates Ligature-Induced, RANKL-Dependent Alveolar Bone Resorption via Differential Regulation of Toll-Like Receptor 2 (TLR2) and TLR4
作者: Lin, Jiang1,2; Bi, Liangjia2; Yu, Xiaoqian1,3; Kawai, Toshihisa1; Taubman, Martin A.1; Shen, Baozhong4; Han, Xiaozhe1
刊名: INFECTION AND IMMUNITY
发表日期: 2014-10-01
DOI: 10.1128/IAI.02084-14
卷: 82, 期:10, 页:4127-4134
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Immunology ; Infectious Diseases
研究领域[WOS]: Immunology ; Infectious Diseases
关键词[WOS]: NF-KAPPA-B ; PERIODONTAL-DISEASE ; IL-10 PRODUCTION ; HEMAGGLUTININ B ; DENDRITIC CELLS ; INNATE IMMUNITY ; HOST RESPONSE ; PATHOGEN ; LIPOPOLYSACCHARIDE ; RECOGNITION
英文摘要:

Toll-like receptors (TLRs) play a key role in the innate immune responses to periodontal pathogens in periodontal disease. The present study was performed to determine the roles of TLR2 and TLR4 signaling in alveolar bone resorption, using a Porphyromonas gingivalis-associated ligature-induced periodontitis model in mice. Wild-type (WT), Tlr2(-/-), and Tlr4(-/-) mice (8 to 10 weeks old) in the C57/BL6 background were used. Silk ligatures were applied to the maxillary second molars in the presence or absence of live P. gingivalis infection. Ligatures were removed from the second molars on day 14, and mice were kept for another 2 weeks before sacrifice for final analysis (day 28). On day 14, there were no differences in alveolar bone resorption and gingival RANKL expression between mice treated with ligation plus P. gingivalis infection and mice treated with ligation alone. Gingival interleukin-1 beta (IL-1 beta) and tumor necrosis factor alpha (TNF-alpha) expression was increased, whereas IL-10 expression was decreased in WT and Tlr2(-/-) mice but not in Tlr4(-/-) mice. On day 28, WT and Tlr4(-/-) mice treated with ligation plus P. gingivalis infection showed significantly increased bone loss and gingival RANKL expression compared to those treated with ligation alone, whereas such an increase was diminished in Tlr2(-/-) mice. Gingival TNF-alpha upregulation and IL-10 downregulation were observed only in WT and Tlr4(-/-) mice, not in Tlr2(-/-) mice. In all mice, bone resorption induced by ligation plus P. gingivalis infection was antagonized by local anti-RANKL antibody administration. This study suggests that P. gingivalis exacerbates ligature- induced, RANKL-dependent periodontal bone resorption via differential regulation of TLR2 and TLR4 signaling.

语种: 英语
所属项目编号: DE-003420 ; DE-021837
项目资助者: NIH from the National Institute of Dental and Craniofacial Research
WOS记录号: WOS:000341935100013
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62702
Appears in Collections:北京大学口腔医学院_牙周科_期刊论文

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作者单位: 1.Forsyth Inst, Dept Immunol & Infect Dis, Cambridge, MA USA
2.Harbin Med Univ, Hosp 4, Dept Stomatol, Harbin, Peoples R China
3.Peking Univ, Sch & Hosp Stomatol, Dept Periodontol, Beijing 100871, Peoples R China
4.Harbin Med Univ, Dept Radiol, Hosp 4, Harbin, Peoples R China

Recommended Citation:
Lin, Jiang,Bi, Liangjia,Yu, Xiaoqian,et al. Porphyromonas gingivalis Exacerbates Ligature-Induced, RANKL-Dependent Alveolar Bone Resorption via Differential Regulation of Toll-Like Receptor 2 (TLR2) and TLR4[J]. INFECTION AND IMMUNITY,2014,82(10):4127-4134.
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