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学科主题: 临床医学
题名:
Rat adipose-derived stromal cells expressing BMP4 induce ectopic bone formation in vitro and in vivo
作者: Lin, Lin; Fu, Xin; Zhang, Xin; Chen, Lian-Xu; Zhang, Ji-Ying; Yu, Chang-Long; Ma, Kang-Tao; Zhou, Chun-Yan
关键词: adipose-derived stromal cells ; BMP4 ; gene therapy ; bone formation
刊名: ACTA PHARMACOLOGICA SINICA
发表日期: 2006-12-01
DOI: 10.1111/j.1745-7254.2006.00449.x
卷: 27, 期:12, 页:1608-1615
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]: Chemistry ; Pharmacology & Pharmacy
关键词[WOS]: ADULT STEM-CELLS ; GENE-THERAPY ; MORPHOGENETIC PROTEIN-2 ; DIFFERENTIATION ; MARROW ; MUSCLE ; REGENERATION ; TRANSIENT ; FRACTURE ; DEFECTS
英文摘要:

Aim: Bone morphogenetic protein 4 (BMP4) is one of the main local contributing factors in callus formation in the early phase of fracture healing. Adipose-derived stromal cells (ADSC) are multipotent cells. The present study was conducted to investigate the osteogenic potential of ADSC when exposed to adenovirus containing BMP4 cDNA (Ad-BMP4). Methods: ADSC were harvested from Sprague-Dawley rats. After exposure to Ad-BMP4, ADSC were assessed by alkaline phosphatase activity (ALP) assay, RT-PCR and von Kossa staining. BMP4 expression was assessed by RT-PCR, immunofluorescence and Western blot analysis. ADSC transduced with Ad-BMP4 were directly injected into the hind limb muscles of athymic mice. ADSC Ad-EGFP(enhanced green fluorescence protein) served as controls. All animals were examined by X-ray film and histological analysis. Results: The expression of BMP4 was confirmed at both mRNA and protein levels. The expression of the osteoblastic gene, ALP activity and von Kossa staining confirmed that ADSC transduced with Ad-BMP4 underwent rapid and marked osteoblast differentiation, whereas ADSC transduced with Ad-EGFP and cells left alone displayed no osteogenic differentiation. X-ray and histological examination confirmed new bone formation in athymic mice transplanted with ADSC transduced with Ad-BMP4. Conclusion: Our data demonstrated successful osteogenic differentiation of ADSC transduced with Ad-BMP4 in vitro and in vivo. ADSC maybe an ideal source of mesenchyme lineage stem cells for gene therapy and tissue engineering.

语种: 英语
WOS记录号: WOS:000242221300013
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62714
Appears in Collections:北京大学第三临床医学院_运动医学研究所_期刊论文

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作者单位: 1.Peking Univ, Third Hosp, Inst Sports Med, Beijing 100083, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Dept Biochem & Mol Biol, Beijing 100083, Peoples R China

Recommended Citation:
Lin, Lin,Fu, Xin,Zhang, Xin,et al. Rat adipose-derived stromal cells expressing BMP4 induce ectopic bone formation in vitro and in vivo[J]. ACTA PHARMACOLOGICA SINICA,2006,27(12):1608-1615.
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