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学科主题临床医学
Rat adipose-derived stromal cells expressing BMP4 induce ectopic bone formation in vitro and in vivo
Lin, Lin; Fu, Xin; Zhang, Xin; Chen, Lian-Xu; Zhang, Ji-Ying; Yu, Chang-Long; Ma, Kang-Tao; Zhou, Chun-Yan
关键词adipose-derived stromal cells BMP4 gene therapy bone formation
刊名ACTA PHARMACOLOGICA SINICA
2006-12-01
DOI10.1111/j.1745-7254.2006.00449.x
27期:12页:1608-1615
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]Chemistry ; Pharmacology & Pharmacy
关键词[WOS]ADULT STEM-CELLS ; GENE-THERAPY ; MORPHOGENETIC PROTEIN-2 ; DIFFERENTIATION ; MARROW ; MUSCLE ; REGENERATION ; TRANSIENT ; FRACTURE ; DEFECTS
英文摘要

Aim: Bone morphogenetic protein 4 (BMP4) is one of the main local contributing factors in callus formation in the early phase of fracture healing. Adipose-derived stromal cells (ADSC) are multipotent cells. The present study was conducted to investigate the osteogenic potential of ADSC when exposed to adenovirus containing BMP4 cDNA (Ad-BMP4). Methods: ADSC were harvested from Sprague-Dawley rats. After exposure to Ad-BMP4, ADSC were assessed by alkaline phosphatase activity (ALP) assay, RT-PCR and von Kossa staining. BMP4 expression was assessed by RT-PCR, immunofluorescence and Western blot analysis. ADSC transduced with Ad-BMP4 were directly injected into the hind limb muscles of athymic mice. ADSC Ad-EGFP(enhanced green fluorescence protein) served as controls. All animals were examined by X-ray film and histological analysis. Results: The expression of BMP4 was confirmed at both mRNA and protein levels. The expression of the osteoblastic gene, ALP activity and von Kossa staining confirmed that ADSC transduced with Ad-BMP4 underwent rapid and marked osteoblast differentiation, whereas ADSC transduced with Ad-EGFP and cells left alone displayed no osteogenic differentiation. X-ray and histological examination confirmed new bone formation in athymic mice transplanted with ADSC transduced with Ad-BMP4. Conclusion: Our data demonstrated successful osteogenic differentiation of ADSC transduced with Ad-BMP4 in vitro and in vivo. ADSC maybe an ideal source of mesenchyme lineage stem cells for gene therapy and tissue engineering.

语种英语
WOS记录号WOS:000242221300013
引用统计
被引频次:33[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62714
专题北京大学第三临床医学院_运动医学研究所
作者单位1.Peking Univ, Third Hosp, Inst Sports Med, Beijing 100083, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Dept Biochem & Mol Biol, Beijing 100083, Peoples R China
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GB/T 7714
Lin, Lin,Fu, Xin,Zhang, Xin,et al. Rat adipose-derived stromal cells expressing BMP4 induce ectopic bone formation in vitro and in vivo[J]. ACTA PHARMACOLOGICA SINICA,2006,27(12):1608-1615.
APA Lin, Lin.,Fu, Xin.,Zhang, Xin.,Chen, Lian-Xu.,Zhang, Ji-Ying.,...&Zhou, Chun-Yan.(2006).Rat adipose-derived stromal cells expressing BMP4 induce ectopic bone formation in vitro and in vivo.ACTA PHARMACOLOGICA SINICA,27(12),1608-1615.
MLA Lin, Lin,et al."Rat adipose-derived stromal cells expressing BMP4 induce ectopic bone formation in vitro and in vivo".ACTA PHARMACOLOGICA SINICA 27.12(2006):1608-1615.
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