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学科主题: 基础医学
题名:
Thermosensitive TRP channel pore turret is part of the temperature activation pathway
作者: Yang, Fan1; Cui, Yuanyuan1,2; Wang, KeWei2; Zheng, Jie1
关键词: conformational change ; fluorescence resonance energy transfer ; temperature sensing ; thermodynamics
刊名: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
发表日期: 2010-04-13
DOI: 10.1073/pnas.1000357107
卷: 107, 期:15, 页:7083-7088
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: NUCLEOTIDE-GATED CHANNELS ; VANILLOID RECEPTOR-I ; ION CHANNELS ; K+ CHANNEL ; POTASSIUM CHANNELS ; CRYSTAL-STRUCTURE ; HEAT ACTIVATION ; MOLECULAR-BASIS ; COLD RECEPTOR ; PATCH-CLAMP
英文摘要:

Temperature sensing is crucial for homeotherms, including human beings, to maintain a stable body core temperature and respond to the ambient environment. A group of exquisitely temperature-sensitive transient receptor potential channels, termed thermoTRPs, serve as cellular temperature sensors. How thermoTRPs convert thermal energy (heat) into protein conformational changes leading to channel opening remains unknown. Here we demonstrate that the pathway for temperature-dependent activation is distinct from those for ligand- and voltage-dependent activation and involves the pore turret. We found that mutant channels with an artificial pore turret sequence lose temperature sensitivity but maintain normal ligand responses. Using site-directed fluorescence recordings we observed that temperature change induces a significant rearrangement of TRPV1 pore turret that is coupled to channel opening. This movement is specifically associated to temperature-dependent activation and is not observed during ligand- and voltage-dependent channel activation. These observations suggest that the turret is part of the temperature-sensing apparatus in thermoTRP channels, and its conformational change may give rise to the large entropy that defines high temperature sensitivity.

语种: 英语
所属项目编号: REY016754A ; 0665201Y ; 30970919 ; B07001
项目资助者: National Institutes of Health ; American Heart Association ; UC Davis Health System Research Award ; National Science Foundation of China ; Ministry of Education of China
WOS记录号: WOS:000276642100093
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62727
Appears in Collections:基础医学院_神经生物学系_期刊论文

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作者单位: 1.Univ Calif Davis, Dept Physiol & Membrane Biol, Davis, CA 95616 USA
2.Peking Univ, Hlth Sci Ctr, Neurosci Res Inst, Dept Neurobiol, Beijing 100083, Peoples R China

Recommended Citation:
Yang, Fan,Cui, Yuanyuan,Wang, KeWei,et al. Thermosensitive TRP channel pore turret is part of the temperature activation pathway[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2010,107(15):7083-7088.
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