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学科主题基础医学
Thermosensitive TRP channel pore turret is part of the temperature activation pathway
Yang, Fan1; Cui, Yuanyuan1,2; Wang, KeWei2; Zheng, Jie1
关键词Conformational Change Fluorescence Resonance Energy Transfer Temperature Sensing Thermodynamics
刊名PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
2010-04-13
DOI10.1073/pnas.1000357107
107期:15页:7083-7088
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]NUCLEOTIDE-GATED CHANNELS ; VANILLOID RECEPTOR-I ; ION CHANNELS ; K+ CHANNEL ; POTASSIUM CHANNELS ; CRYSTAL-STRUCTURE ; HEAT ACTIVATION ; MOLECULAR-BASIS ; COLD RECEPTOR ; PATCH-CLAMP
英文摘要

Temperature sensing is crucial for homeotherms, including human beings, to maintain a stable body core temperature and respond to the ambient environment. A group of exquisitely temperature-sensitive transient receptor potential channels, termed thermoTRPs, serve as cellular temperature sensors. How thermoTRPs convert thermal energy (heat) into protein conformational changes leading to channel opening remains unknown. Here we demonstrate that the pathway for temperature-dependent activation is distinct from those for ligand- and voltage-dependent activation and involves the pore turret. We found that mutant channels with an artificial pore turret sequence lose temperature sensitivity but maintain normal ligand responses. Using site-directed fluorescence recordings we observed that temperature change induces a significant rearrangement of TRPV1 pore turret that is coupled to channel opening. This movement is specifically associated to temperature-dependent activation and is not observed during ligand- and voltage-dependent channel activation. These observations suggest that the turret is part of the temperature-sensing apparatus in thermoTRP channels, and its conformational change may give rise to the large entropy that defines high temperature sensitivity.

语种英语
WOS记录号WOS:000276642100093
项目编号REY016754A ; 0665201Y ; 30970919 ; B07001
资助机构National Institutes of Health ; American Heart Association ; UC Davis Health System Research Award ; National Science Foundation of China ; Ministry of Education of China
引用统计
被引频次:99[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62727
专题北京大学基础医学院_神经生物学系
北京大学药学院_分子与细胞药理学系
作者单位1.Univ Calif Davis, Dept Physiol & Membrane Biol, Davis, CA 95616 USA
2.Peking Univ, Hlth Sci Ctr, Neurosci Res Inst, Dept Neurobiol, Beijing 100083, Peoples R China
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GB/T 7714
Yang, Fan,Cui, Yuanyuan,Wang, KeWei,et al. Thermosensitive TRP channel pore turret is part of the temperature activation pathway[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2010,107(15):7083-7088.
APA Yang, Fan,Cui, Yuanyuan,Wang, KeWei,&Zheng, Jie.(2010).Thermosensitive TRP channel pore turret is part of the temperature activation pathway.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,107(15),7083-7088.
MLA Yang, Fan,et al."Thermosensitive TRP channel pore turret is part of the temperature activation pathway".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 107.15(2010):7083-7088.
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