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学科主题: 临床医学
题名:
Excess Integrins Cause Lung Entrapment of Mesenchymal Stem Cells
作者: Wang, Shan1,2; Guo, Ling2; Ge, Jianfeng1,2; Yu, Lin3,4; Cai, Ting1,2; Tian, Ruiyun2; Jiang, Yuyang5; Zhao, Robert C. H.6,7; Wu, Yaojiong2
关键词: Mesenchymal stem cells ; Integrins ; Lung entrapment ; Homing ; Vascular obstructions
刊名: STEM CELLS
发表日期: 2015-11-01
DOI: 10.1002/stem.2087
卷: 33, 期:11, 页:3315-3326
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell & Tissue Engineering ; Biotechnology & Applied Microbiology ; Oncology ; Cell Biology ; Hematology
研究领域[WOS]: Cell Biology ; Biotechnology & Applied Microbiology ; Oncology ; Hematology
关键词[WOS]: MARROW STROMAL CELLS ; MYOCARDIAL-INFARCTION ; DELIVERY ; INJECTION ; THERAPY ; STROKE ; DIFFERENTIATION ; ANGIOGENESIS ; ISCHEMIA ; BEHAVIOR
英文摘要:

Mesenchymal stem cells (MSCs) are largely entrapped in the lungs after intravenous delivery. The underlying mechanisms have been poorly understood. Flow cytometry and Western blot analysis showed that the expression levels of many integrins such as beta 1, alpha 5, and alpha V beta 3 in MSCs increased markedly upon cultured expansion in 2D monolayers, whose ligands fibronectin and vitronectin were detected on the surface of vascular endothelial cells in the lungs by immunostaining and flow cytometry. Blockade of integrin beta 1, integrin alpha 5, or integrins alpha V beta 3 with functional blocking antibodies significantly decreased the amount of MSCs entrapped in the lungs following intravenous infusion as determined by real-time PCR and histological analysis; meanwhile, corresponding increases in the levels of circulating MSCs in the blood and MSCs homed to the ischemic myocardium and inflamed ear were found. Intriguingly, a short period of 3D spheroid culture of MSCs, which had been expanded for several passages in monolayers, substantially reduced the expression levels of many integrins and the number of MSCs entrapped in the lungs. Our results indicate that the excess expression and activation of integrins is a significant cause of lung entrapment of MSCs.

语种: 英语
所属项目编号: 31371404 ; JCYJ20130402145002397 ; JCYJ20140417115840279
项目资助者: Natural Science Foundation of China ; Shenzhen Science and Technology Innovation Committee
WOS记录号: WOS:000363265900015
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/62732
Appears in Collections:北京大学深圳医院_期刊论文

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作者单位: 1.Shenzhen Key Lab Gynecol Diagnost Technol Res, Shenzhen, Peoples R China
2.Sch Basic Med, Beijing, Peoples R China
3.Tsinghua Univ, Sch Life Sci, Beijing, Peoples R China
4.Peking Univ, Shenzhen Hosp, Beijing, Peoples R China
5.Tsinghua Univ, Grad Sch Shenzhen, Shenzhen Key Lab Hlth Sci & Technol, Shenzhen 518057, Peoples R China
6.Tsinghua Univ, State Key Lab Breeding Base, Shenzhen Key Lab Chem Biol, Grad Sch Shenzhen, Beijing, Peoples R China
7.Chinese Acad Med Sci, Dept Cell Biol, Inst Basic Med Sci, Beijing 100730, Peoples R China

Recommended Citation:
Wang, Shan,Guo, Ling,Ge, Jianfeng,et al. Excess Integrins Cause Lung Entrapment of Mesenchymal Stem Cells[J]. STEM CELLS,2015,33(11):3315-3326.
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