IR@PKUHSC  > 北京大学深圳医院
学科主题临床医学
Excess Integrins Cause Lung Entrapment of Mesenchymal Stem Cells
Wang, Shan1,2; Guo, Ling2; Ge, Jianfeng1,2; Yu, Lin3,4; Cai, Ting1,2; Tian, Ruiyun2; Jiang, Yuyang5; Zhao, Robert C. H.6,7; Wu, Yaojiong2
关键词Mesenchymal stem cells Integrins Lung entrapment Homing Vascular obstructions
刊名STEM CELLS
2015-11-01
DOI10.1002/stem.2087
33期:11页:3315-3326
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell & Tissue Engineering ; Biotechnology & Applied Microbiology ; Oncology ; Cell Biology ; Hematology
研究领域[WOS]Cell Biology ; Biotechnology & Applied Microbiology ; Oncology ; Hematology
关键词[WOS]MARROW STROMAL CELLS ; MYOCARDIAL-INFARCTION ; DELIVERY ; INJECTION ; THERAPY ; STROKE ; DIFFERENTIATION ; ANGIOGENESIS ; ISCHEMIA ; BEHAVIOR
英文摘要

Mesenchymal stem cells (MSCs) are largely entrapped in the lungs after intravenous delivery. The underlying mechanisms have been poorly understood. Flow cytometry and Western blot analysis showed that the expression levels of many integrins such as beta 1, alpha 5, and alpha V beta 3 in MSCs increased markedly upon cultured expansion in 2D monolayers, whose ligands fibronectin and vitronectin were detected on the surface of vascular endothelial cells in the lungs by immunostaining and flow cytometry. Blockade of integrin beta 1, integrin alpha 5, or integrins alpha V beta 3 with functional blocking antibodies significantly decreased the amount of MSCs entrapped in the lungs following intravenous infusion as determined by real-time PCR and histological analysis; meanwhile, corresponding increases in the levels of circulating MSCs in the blood and MSCs homed to the ischemic myocardium and inflamed ear were found. Intriguingly, a short period of 3D spheroid culture of MSCs, which had been expanded for several passages in monolayers, substantially reduced the expression levels of many integrins and the number of MSCs entrapped in the lungs. Our results indicate that the excess expression and activation of integrins is a significant cause of lung entrapment of MSCs.

语种英语
WOS记录号WOS:000363265900015
项目编号31371404 ; JCYJ20130402145002397 ; JCYJ20140417115840279
资助机构Natural Science Foundation of China ; Shenzhen Science and Technology Innovation Committee
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62732
专题北京大学深圳医院
作者单位1.Shenzhen Key Lab Gynecol Diagnost Technol Res, Shenzhen, Peoples R China
2.Sch Basic Med, Beijing, Peoples R China
3.Tsinghua Univ, Sch Life Sci, Beijing, Peoples R China
4.Peking Univ, Shenzhen Hosp, Beijing, Peoples R China
5.Tsinghua Univ, Grad Sch Shenzhen, Shenzhen Key Lab Hlth Sci & Technol, Shenzhen 518057, Peoples R China
6.Tsinghua Univ, State Key Lab Breeding Base, Shenzhen Key Lab Chem Biol, Grad Sch Shenzhen, Beijing, Peoples R China
7.Chinese Acad Med Sci, Dept Cell Biol, Inst Basic Med Sci, Beijing 100730, Peoples R China
推荐引用方式
GB/T 7714
Wang, Shan,Guo, Ling,Ge, Jianfeng,et al. Excess Integrins Cause Lung Entrapment of Mesenchymal Stem Cells[J]. STEM CELLS,2015,33(11):3315-3326.
APA Wang, Shan.,Guo, Ling.,Ge, Jianfeng.,Yu, Lin.,Cai, Ting.,...&Wu, Yaojiong.(2015).Excess Integrins Cause Lung Entrapment of Mesenchymal Stem Cells.STEM CELLS,33(11),3315-3326.
MLA Wang, Shan,et al."Excess Integrins Cause Lung Entrapment of Mesenchymal Stem Cells".STEM CELLS 33.11(2015):3315-3326.
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