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Changes of conformation and aggregation state induced by binding of lanthanide ions to insulin
Cheng, Y; Li, RC; Wang, K
关键词Insulin Lanthanide Conformation Aggregation
刊名SCIENCE IN CHINA SERIES B-CHEMISTRY
2002-08-01
45期:4页:349-357
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Multidisciplinary
研究领域[WOS]Chemistry
关键词[WOS]BOVINE INSULIN ; ZINC-BINDING ; DERIVATIVES ; RECEPTOR ; HEXAMER
英文摘要

To clarify the mechanism of lanthanide ions (Ln(3+)) on the across-membrane transport of insulin and subsequent reducing blood glucose, the interactions of Ln(3+) with Zn-insulin and Zn-free insulin are investigated by spectroscopic methods. The results reveal that the binding of Ln(3+) to insulin can induce its structure changes from secondary to quaternary structure, depending on the Ln(3+) concentration. In the lower concentration, it triggers the conformational changes of insulin monomer in the binding region with insulin receptor (B(24-30)). It would affect insulin-insulin receptor interaction. Moreover, Ln(3+) binding promotes the assembly of insulin monomer from dimer to polymer. The potency of Ln(3+) in inducing insulin′s aggregation is stronger than that of Zn2+. Furthermore, the aggregation can be reversed partly by EDTA-treatment, indicating that it is not due to denaturation. Similar to Zn2+ effect, Ln(3+) can stabilize insulin hexamer in a certain range of concentration, but is stronger than the former.

语种英语
WOS记录号WOS:000177050600002
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/62754
专题北京大学药学院_化学生物学系
作者单位Peking Univ, Sch Pharmaceut Sci, Dept Biol Chem, Beijing 100083, Peoples R China
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GB/T 7714
Cheng, Y,Li, RC,Wang, K. Changes of conformation and aggregation state induced by binding of lanthanide ions to insulin[J]. SCIENCE IN CHINA SERIES B-CHEMISTRY,2002,45(4):349-357.
APA Cheng, Y,Li, RC,&Wang, K.(2002).Changes of conformation and aggregation state induced by binding of lanthanide ions to insulin.SCIENCE IN CHINA SERIES B-CHEMISTRY,45(4),349-357.
MLA Cheng, Y,et al."Changes of conformation and aggregation state induced by binding of lanthanide ions to insulin".SCIENCE IN CHINA SERIES B-CHEMISTRY 45.4(2002):349-357.
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